(Linear) (Six-base) MAPSORT of: KIEE2277.seq  Check: 4495  from: 1  to: 390



KIEE2277.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:56  ..

ApaLI G'TGCA_C

Cuts at:      0      91     390 
   Size:         91     299
 
AscI GG'CGCG_CC

Cuts at:      0     140     390 
   Size:        140     250
 
BanII G_rGCy'C

Cuts at:      0     281     390 
   Size:        281     109
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     232     390 
   Size:        232     158
 
Bme1580I G_kGCm'C

Cuts at:      0      95     390 
   Size:         95     295
 
BmgBI CAC'GTC

Cuts at:      0      10     390 
   Size:         10     380
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     187     390 
   Size:         55     132     203

  Fragments arranged by size: 

                 203     132      55
 
Bpu10I CC'TnA_GC

Cuts at:      0      38     390 
   Size:         38     352
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      73     390 
   Size:         73     317
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     289     390 
   Size:        289     101
 
BseYI C'CCAG_C

Cuts at:      0     291     390 
   Size:        291      99
 
BsiHKAI G_wGCw'C

Cuts at:      0      95     281     390 
   Size:         95     186     109

  Fragments arranged by size: 

                 186     109      95
 
Bsp1286I G_dGCh'C

Cuts at:      0      95     281     390 
   Size:         95     186     109

  Fragments arranged by size: 

                 186     109      95
 
BsrBI CCG'CTC

Cuts at:      0     126     390 
   Size:        126     264
 
BsrFI r'CCGG_y

Cuts at:      0      64     136     144     390 
   Size:         64      72       8     246

  Fragments arranged by size: 

                 246      72      64       8
 
BssHII G'CGCG_C

Cuts at:      0     140     390 
   Size:        140     250
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      99     390 
   Size:         99     291
 
BstYI r'GATC_y

Cuts at:      0     185     390 
   Size:        185     205
 
BtgI C'CryG_G

Cuts at:      0      80     390 
   Size:         80     310
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     291     390 
   Size:        291      99
 
EaeI y'GGCC_r

Cuts at:      0     257     390 
   Size:        257     133
 
EarI CTCTTCn'nnn_

Cuts at:      0      60     390 
   Size:         60     330
 
EcoICRI GAG'CTC

Cuts at:      0     279     390 
   Size:        279     111
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     187     390 
   Size:         55     132     203

  Fragments arranged by size: 

                 203     132      55
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     276     390 
   Size:        276     114
 
MscI TGG'CCA

Cuts at:      0     259     390 
   Size:        259     131
 
MspA1I CmG'CkG

Cuts at:      0     160     390 
   Size:        160     230
 
NaeI GCC'GGC

Cuts at:      0     138     390 
   Size:        138     252
 
NgoMIV G'CCGG_C

Cuts at:      0     136     390 
   Size:        136     254
 
PfoI T'CCnGG_A

Cuts at:      0     188     390 
   Size:        188     202
 
SacI G_AGCT'C

Cuts at:      0     281     390 
   Size:        281     109
 
SapI GCTCTTCn'nnn_

Cuts at:      0      60     390 
   Size:         60     330
 
SgrAI Cr'CCGG_yG

Cuts at:      0     144     390 
   Size:        144     246
 

 Enzymes that do cut:

    ApaLI     AscI    BanII     BbsI Bme1580I    BmgBI     BpmI   Bpu10I
     BsaI    BseRI    BseYI  BsiHKAI Bsp1286I    BsrBI    BsrFI   BssHII
   BstAPI    BstYI     BtgI    BtgZI     EaeI     EarI  EcoICRI  Eco57MI
     MmeI     MscI   MspA1I     NaeI   NgoMIV     PfoI     SacI     SapI
    SgrAI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
     ApoI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrDI    BsrGI    BssSI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MslI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI