(Linear) (Six-base) MAPSORT of: KIEE2272.seq  Check: 2296  from: 1  to: 639



KIEE2272.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:56  ..

AccI GT'mk_AC

Cuts at:      0     256     307     639 
   Size:        256      51     332

  Fragments arranged by size: 

                 332     256      51
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     434     639 
   Size:        434     205
 
ApoI r'AATT_y

Cuts at:      0     276     378     518     639 
   Size:        276     102     140     121

  Fragments arranged by size: 

                 276     140     121     102
 
BamHI G'GATC_C

Cuts at:      0     348     639 
   Size:        348     291
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     220     639 
   Size:        220     419
 
BclI T'GATC_A

Cuts at:      0     422     639 
   Size:        422     217
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317     639 
   Size:        317     322
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     604     639 
   Size:        604      35
 
BsaHI Gr'CG_yC

Cuts at:      0       4     639 
   Size:          4     635
 
BsrDI GCAATG_nn'

Cuts at:      0     308     639 
   Size:        308     331
 
BstYI r'GATC_y

Cuts at:      0     348     639 
   Size:        348     291
 
BtgI C'CryG_G

Cuts at:      0      26     639 
   Size:         26     613
 
EaeI y'GGCC_r

Cuts at:      0     146     533     639 
   Size:        146     387     106

  Fragments arranged by size: 

                 387     146     106
 
EarI CTCTTCn'nnn_

Cuts at:      0      16     596     639 
   Size:         16     580      43

  Fragments arranged by size: 

                 580      43      16
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317     434     639 
   Size:        317     117     205

  Fragments arranged by size: 

                 317     205     117
 
EcoRI G'AATT_C

Cuts at:      0     276     639 
   Size:        276     363
 
HincII GTy'rAC

Cuts at:      0     336     639 
   Size:        336     303
 
HindIII A'AGCT_T

Cuts at:      0     167     558     639 
   Size:        167     391      81

  Fragments arranged by size: 

                 391     167      81
 
HpaI GTT'AAC

Cuts at:      0     336     639 
   Size:        336     303
 
MscI TGG'CCA

Cuts at:      0     148     535     639 
   Size:        148     387     104

  Fragments arranged by size: 

                 387     148     104
 
MslI CAynn'nnrTG

Cuts at:      0     400     639 
   Size:        400     239
 
MspA1I CmG'CkG

Cuts at:      0     123     550     639 
   Size:        123     427      89

  Fragments arranged by size: 

                 427     123      89
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     602     634     639 
   Size:        602      32       5

  Fragments arranged by size: 

                 602      32       5
 
PvuII CAG'CTG

Cuts at:      0     123     550     639 
   Size:        123     427      89

  Fragments arranged by size: 

                 427     123      89
 
SmlI C'TyrA_G

Cuts at:      0     619     639 
   Size:        619      20
 

 Enzymes that do cut:

     AccI     AcuI     ApoI    BamHI     BbsI     BclI     BpmI    BpuEI
    BsaHI    BsrDI    BstYI     BtgI     EaeI     EarI  Eco57MI    EcoRI
   HincII  HindIII     HpaI     MscI     MslI   MspA1I     PpiI    PvuII
     SmlI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI