(Linear) (Six-base) MAPSORT of: KIEE2268.seq  Check: 8716  from: 1  to: 384



KIEE2268.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:56  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     196     384 
   Size:        196     188
 
AlwNI CAG_nnn'CTG

Cuts at:      0     308     384 
   Size:        308      76
 
ApaLI G'TGCA_C

Cuts at:      0     181     384 
   Size:        181     203
 
AvaI C'yCGr_G

Cuts at:      0     209     274     384 
   Size:        209      65     110

  Fragments arranged by size: 

                 209     110      65
 
BamHI G'GATC_C

Cuts at:      0      50     384 
   Size:         50     334
 
BanI G'GyrC_C

Cuts at:      0      30     384 
   Size:         30     354
 
Bme1580I G_kGCm'C

Cuts at:      0      35     185     384 
   Size:         35     150     199

  Fragments arranged by size: 

                 199     150      35
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     237     384 
   Size:        237     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94     213     384 
   Size:         94     119     171

  Fragments arranged by size: 

                 171     119      94
 
BseYI C'CCAG_C

Cuts at:      0     169     245     384 
   Size:        169      76     139

  Fragments arranged by size: 

                 169     139      76
 
BsiHKAI G_wGCw'C

Cuts at:      0     185     384 
   Size:        185     199
 
Bsp1286I G_dGCh'C

Cuts at:      0      35     185     384 
   Size:         35     150     199

  Fragments arranged by size: 

                 199     150      35
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      61     384 
   Size:         61     323
 
BstYI r'GATC_y

Cuts at:      0      50     384 
   Size:         50     334
 
BtgI C'CryG_G

Cuts at:      0     324     384 
   Size:        324      60
 
BtsI GCAGTG_nn'

Cuts at:      0     206     384 
   Size:        206     178
 
EaeI y'GGCC_r

Cuts at:      0      39     228     327     384 
   Size:         39     189      99      57

  Fragments arranged by size: 

                 189      99      57      39
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     126     384 
   Size:        126     258
 
EcoO109I rG'GnC_Cy

Cuts at:      0     143     384 
   Size:        143     241
 
HindIII A'AGCT_T

Cuts at:      0      11     384 
   Size:         11     373
 
MscI TGG'CCA

Cuts at:      0     329     384 
   Size:        329      55
 
MslI CAynn'nnrTG

Cuts at:      0     178     384 
   Size:        178     206
 
MspA1I CmG'CkG

Cuts at:      0     249     295     351     384 
   Size:        249      46      56      33

  Fragments arranged by size: 

                 249      56      46      33
 
NcoI C'CATG_G

Cuts at:      0     324     384 
   Size:        324      60
 
PasI CC'CwG_GG

Cuts at:      0     150     384 
   Size:        150     234
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     308     384 
   Size:        308      76
 
PshAI GACnn'nnGTC

Cuts at:      0     103     384 
   Size:        103     281
 
PvuII CAG'CTG

Cuts at:      0     249     351     384 
   Size:        249     102      33

  Fragments arranged by size: 

                 249     102      33
 
SmaI CCC'GGG

Cuts at:      0     211     276     384 
   Size:        211      65     108

  Fragments arranged by size: 

                 211     108      65
 
StuI AGG'CCT

Cuts at:      0      60     384 
   Size:         60     324
 
StyI C'CwwG_G

Cuts at:      0     324     384 
   Size:        324      60
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      84     384 
   Size:         84     300
 
XmaI C'CCGG_G

Cuts at:      0     209     274     384 
   Size:        209      65     110

  Fragments arranged by size: 

                 209     110      65
 

 Enzymes that do cut:

     AlfI    AlwNI    ApaLI     AvaI    BamHI     BanI Bme1580I     BplI
    BseRI    BseYI  BsiHKAI Bsp1286I    BstXI    BstYI     BtgI     BtsI
     EaeI    EcoNI EcoO109I  HindIII     MscI     MslI   MspA1I     NcoI
     PasI    PflMI    PshAI    PvuII     SmaI     StuI     StyI    TaqII
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI     ApaI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI