Restriction map for
pFN21AE2266
(Linear) (Six-base) MAPSORT of: KIEE2266.seq Check: 4386 from: 1 to: 2556
KIEE2266.orf
With 182 enzymes: SgfI *
July 15, 2010 18:56 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 612 671 1205 1334 1948 2556
Size: 592 20 59 534 129 614 608
Fragments arranged by size:
614 608 592 534 129 59 20
AflIII A'CryG_T
Cuts at: 0 276 874 1207 2281 2556
Size: 276 598 333 1074 275
Fragments arranged by size:
1074 598 333 276 275
AleI CACnn'nnGTG
Cuts at: 0 58 1006 2263 2556
Size: 58 948 1257 293
Fragments arranged by size:
1257 948 293 58
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2325 2357 2556
Size: 2325 32 199
Fragments arranged by size:
2325 199 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1239 2133 2556
Size: 1239 894 423
Fragments arranged by size:
1239 894 423
ApoI r'AATT_y
Cuts at: 0 701 823 2556
Size: 701 122 1733
Fragments arranged by size:
1733 701 122
AseI AT'TA_AT
Cuts at: 0 1363 2556
Size: 1363 1193
AvaI C'yCGr_G
Cuts at: 0 14 2556
Size: 14 2542
BanI G'GyrC_C
Cuts at: 0 475 991 2556
Size: 475 516 1565
Fragments arranged by size:
1565 516 475
BanII G_rGCy'C
Cuts at: 0 15 899 1136 1702 2556
Size: 15 884 237 566 854
Fragments arranged by size:
884 854 566 237 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 606 1672 2556
Size: 606 1066 884
Fragments arranged by size:
1066 884 606
BciVI GTATCCnnnnn_n'
Cuts at: 0 1969 2556
Size: 1969 587
BclI T'GATC_A
Cuts at: 0 2309 2556
Size: 2309 247
BglII A'GATC_T
Cuts at: 0 91 2556
Size: 91 2465
BlpI GC'TnA_GC
Cuts at: 0 1109 2134 2556
Size: 1109 1025 422
Fragments arranged by size:
1109 1025 422
BmgBI CAC'GTC
Cuts at: 0 875 1210 1896 2556
Size: 875 335 686 660
Fragments arranged by size:
875 686 660 335
BmrI ACTGGGnnnn_n'
Cuts at: 0 41 1705 2516 2556
Size: 41 1664 811 40
Fragments arranged by size:
1664 811 41 40
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2033 2556
Size: 2033 523
Bpu10I CC'TnA_GC
Cuts at: 0 1130 2556
Size: 1130 1426
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1536 2154 2556
Size: 1536 618 402
Fragments arranged by size:
1536 618 402
BsaI GGTCTCn'nnnn_
Cuts at: 0 1288 2556
Size: 1288 1268
BsaAI yAC'GTr
Cuts at: 0 2284 2556
Size: 2284 272
BsaBI GATnn'nnATC
Cuts at: 0 562 1768 2556
Size: 562 1206 788
Fragments arranged by size:
1206 788 562
BsaWI w'CCGG_w
Cuts at: 0 803 2556
Size: 803 1753
BseYI C'CCAG_C
Cuts at: 0 707 2457 2556
Size: 707 1750 99
Fragments arranged by size:
1750 707 99
BsiHKAI G_wGCw'C
Cuts at: 0 1136 1702 2019 2556
Size: 1136 566 317 537
Fragments arranged by size:
1136 566 537 317
BsmI GAATG_Cn'
Cuts at: 0 466 1646 2507 2556
Size: 466 1180 861 49
Fragments arranged by size:
1180 861 466 49
BsmBI CGTCTCn'nnnn_
Cuts at: 0 805 1216 2556
Size: 805 411 1340
Fragments arranged by size:
1340 805 411
Bsp1286I G_dGCh'C
Cuts at: 0 15 899 1136 1702 2019 2556
Size: 15 884 237 566 317 537
Fragments arranged by size:
884 566 537 317 237 15
BspEI T'CCGG_A
Cuts at: 0 803 2556
Size: 803 1753
BspHI T'CATG_A
Cuts at: 0 827 2006 2043 2556
Size: 827 1179 37 513
Fragments arranged by size:
1179 827 513 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1519 2556
Size: 1519 1037
BsrDI GCAATG_nn'
Cuts at: 0 82 135 2556
Size: 82 53 2421
Fragments arranged by size:
2421 82 53
BsrFI r'CCGG_y
Cuts at: 0 1226 2556
Size: 1226 1330
BsrGI T'GTAC_A
Cuts at: 0 2238 2556
Size: 2238 318
BstBI TT'CG_AA
Cuts at: 0 1419 2556
Size: 1419 1137
BstXI CCAn_nnnn'nTGG
Cuts at: 0 60 1251 2556
Size: 60 1191 1305
Fragments arranged by size:
1305 1191 60
BstYI r'GATC_y
Cuts at: 0 91 152 635 2556
Size: 91 61 483 1921
Fragments arranged by size:
1921 483 91 61
BtgI C'CryG_G
Cuts at: 0 1535 2556
Size: 1535 1021
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 18 2556
Size: 18 2538
BtsI GCAGTG_nn'
Cuts at: 0 2145 2556
Size: 2145 411
DraI TTT'AAA
Cuts at: 0 1436 1841 2556
Size: 1436 405 715
Fragments arranged by size:
1436 715 405
DraIII CAC_nnn'GTG
Cuts at: 0 1943 2556
Size: 1943 613
DrdI GACnn_nn'nnGTC
Cuts at: 0 1711 2556
Size: 1711 845
EaeI y'GGCC_r
Cuts at: 0 1241 1335 2437 2556
Size: 1241 94 1102 119
Fragments arranged by size:
1241 1102 119 94
EarI CTCTTCn'nnn_
Cuts at: 0 3 568 1081 1515 2551 2556
Size: 3 565 513 434 1036 5
Fragments arranged by size:
1036 565 513 434 5 3
EcoICRI GAG'CTC
Cuts at: 0 1134 1700 2556
Size: 1134 566 856
Fragments arranged by size:
1134 856 566
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 612 671 1205 1334 1948 2033 2556
Size: 592 20 59 534 129 614 85 523
Fragments arranged by size:
614 592 534 523 129 85 59 20
EcoRI G'AATT_C
Cuts at: 0 823 2556
Size: 823 1733
EcoRV GAT'ATC
Cuts at: 0 179 1325 2556
Size: 179 1146 1231
Fragments arranged by size:
1231 1146 179
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2505 2556
Size: 2505 51
HaeII r_GCGC'y
Cuts at: 0 1239 2556
Size: 1239 1317
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 388 420 571 603 2556
Size: 388 32 151 32 1953
Fragments arranged by size:
1953 388 151 32 32
HincII GTy'rAC
Cuts at: 0 1384 1926 2556
Size: 1384 542 630
Fragments arranged by size:
1384 630 542
HpaI GTT'AAC
Cuts at: 0 1926 2556
Size: 1926 630
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 1887 2556
Size: 476 1411 669
Fragments arranged by size:
1411 669 476
MscI TGG'CCA
Cuts at: 0 1243 2556
Size: 1243 1313
MslI CAynn'nnrTG
Cuts at: 0 58 745 1006 1762 2263 2556
Size: 58 687 261 756 501 293
Fragments arranged by size:
756 687 501 293 261 58
MspA1I CmG'CkG
Cuts at: 0 1182 1588 1635 1955 2097 2130 2556
Size: 1182 406 47 320 142 33 426
Fragments arranged by size:
1182 426 406 320 142 47 33
NaeI GCC'GGC
Cuts at: 0 1228 2556
Size: 1228 1328
NcoI C'CATG_G
Cuts at: 0 1535 2556
Size: 1535 1021
NdeI CA'TA_TG
Cuts at: 0 1378 2393 2556
Size: 1378 1015 163
Fragments arranged by size:
1378 1015 163
NgoMIV G'CCGG_C
Cuts at: 0 1226 2556
Size: 1226 1330
NspI r_CATG'y
Cuts at: 0 280 2208 2556
Size: 280 1928 348
Fragments arranged by size:
1928 348 280
PciI A'CATG_T
Cuts at: 0 276 2556
Size: 276 2280
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 282 314 2556
Size: 282 32 2242
Fragments arranged by size:
2242 282 32
PvuII CAG'CTG
Cuts at: 0 1182 1588 1635 2097 2130 2556
Size: 1182 406 47 462 33 426
Fragments arranged by size:
1182 462 426 406 47 33
SacI G_AGCT'C
Cuts at: 0 1136 1702 2556
Size: 1136 566 854
Fragments arranged by size:
1136 854 566
SapI GCTCTTCn'nnn_
Cuts at: 0 3 2556
Size: 3 2553
SexAI A'CCwGG_T
Cuts at: 0 1456 1939 2556
Size: 1456 483 617
Fragments arranged by size:
1456 617 483
SmlI C'TyrA_G
Cuts at: 0 1515 2169 2556
Size: 1515 654 387
Fragments arranged by size:
1515 654 387
SphI G_CATG'C
Cuts at: 0 2208 2556
Size: 2208 348
SspI AAT'ATT
Cuts at: 0 1361 1693 2556
Size: 1361 332 863
Fragments arranged by size:
1361 863 332
StyI C'CwwG_G
Cuts at: 0 1409 1535 2556
Size: 1409 126 1021
Fragments arranged by size:
1409 1021 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 883 2556
Size: 883 1673
TatI w'GTAC_w
Cuts at: 0 1572 2036 2238 2426 2556
Size: 1572 464 202 188 130
Fragments arranged by size:
1572 464 202 188 130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 299 331 703 735 1532 1564 2556
Size: 299 32 372 32 797 32 992
Fragments arranged by size:
992 797 372 299 32 32 32
XbaI T'CTAG_A
Cuts at: 0 406 2556
Size: 406 2150
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 267 2556
Size: 267 2289
XmnI GAAnn'nnTTC
Cuts at: 0 2002 2556
Size: 2002 554
Enzymes that do cut:
AcuI AflIII AleI AloI AlwNI ApoI AseI AvaI
BanI BanII BbsI BciVI BclI BglII BlpI BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI
BseYI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BsrGI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI
EcoRI EcoRV FalI HaeII Hin4I HincII HpaI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI
PciI PsrI PvuII SacI SapI SexAI SmlI SphI
SspI StyI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BfrBI BglI Bme1580I BmtI
BplI BsaHI BsaXI BseRI BsgI BsiEI BsiWI BsrBI
BssHII BssSI BstAPI BstEII BstZ17I Bsu36I ClaI CspCI
EagI EciI EcoNI EcoO109I FseI FspI FspAI HindIII
KasI KpnI MfeI MluI NarI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI RsrII
SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI
Tth111I XhoI XmaI ZraI