Restriction map for
pFN21AE2261
(Linear) (Six-base) MAPSORT of: KIEE2261.seq Check: 8872 from: 1 to: 2178
KIEE2261.orf
With 182 enzymes: SgfI *
March 22, 2012 16:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1390 2178
Size: 1390 788
AccI GT'mk_AC
Cuts at: 0 191 2178
Size: 191 1987
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 818 857 1283 1751 2178
Size: 118 700 39 426 468 427
Fragments arranged by size:
700 468 427 426 118 39
AflIII A'CryG_T
Cuts at: 0 306 2178
Size: 306 1872
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 436 2178
Size: 436 1742
ApaI G_GGCC'C
Cuts at: 0 1928 2178
Size: 1928 250
ApaLI G'TGCA_C
Cuts at: 0 477 2178
Size: 477 1701
ApoI r'AATT_y
Cuts at: 0 240 1059 1800 2044 2178
Size: 240 819 741 244 134
Fragments arranged by size:
819 741 244 240 134
AvaI C'yCGr_G
Cuts at: 0 44 2097 2178
Size: 44 2053 81
Fragments arranged by size:
2053 81 44
BanII G_rGCy'C
Cuts at: 0 871 1928 2178
Size: 871 1057 250
Fragments arranged by size:
1057 871 250
BbsI GAAGACnn'nnnn_
Cuts at: 0 1412 2178
Size: 1412 766
BclI T'GATC_A
Cuts at: 0 450 1080 1755 2178
Size: 450 630 675 423
Fragments arranged by size:
675 630 450 423
BfrBI ATG'CAT
Cuts at: 0 198 1111 1255 2178
Size: 198 913 144 923
Fragments arranged by size:
923 913 198 144
BglII A'GATC_T
Cuts at: 0 393 2101 2178
Size: 393 1708 77
Fragments arranged by size:
1708 393 77
Bme1580I G_kGCm'C
Cuts at: 0 481 1928 2010 2178
Size: 481 1447 82 168
Fragments arranged by size:
1447 481 168 82
BmrI ACTGGGnnnn_n'
Cuts at: 0 631 1387 1619 1940 2178
Size: 631 756 232 321 238
Fragments arranged by size:
756 631 321 238 232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 1240 1760 2178
Size: 236 1004 520 418
Fragments arranged by size:
1004 520 418 236
BsaHI Gr'CG_yC
Cuts at: 0 677 2178
Size: 677 1501
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1733 1763 2178
Size: 1733 30 415
Fragments arranged by size:
1733 415 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 298 1447 2085 2141 2178
Size: 298 1149 638 56 37
Fragments arranged by size:
1149 638 298 56 37
BseYI C'CCAG_C
Cuts at: 0 1882 2178
Size: 1882 296
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 944 2178
Size: 944 1234
BsiHKAI G_wGCw'C
Cuts at: 0 481 871 1517 2178
Size: 481 390 646 661
Fragments arranged by size:
661 646 481 390
BsmI GAATG_Cn'
Cuts at: 0 599 2178
Size: 599 1579
BsmBI CGTCTCn'nnnn_
Cuts at: 0 165 2178
Size: 165 2013
Bsp1286I G_dGCh'C
Cuts at: 0 481 871 1517 1928 2010 2178
Size: 481 390 646 411 82 168
Fragments arranged by size:
646 481 411 390 168 82
BspHI T'CATG_A
Cuts at: 0 1284 2178
Size: 1284 894
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 880 1390 2178
Size: 880 510 788
Fragments arranged by size:
880 788 510
BsrBI CCG'CTC
Cuts at: 0 1344 2178
Size: 1344 834
BsrDI GCAATG_nn'
Cuts at: 0 884 2178
Size: 884 1294
BssSI C'ACGA_G
Cuts at: 0 1895 2178
Size: 1895 283
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1261 2178
Size: 1261 917
BstBI TT'CG_AA
Cuts at: 0 1909 2178
Size: 1909 269
BstXI CCAn_nnnn'nTGG
Cuts at: 0 300 2178
Size: 300 1878
BstYI r'GATC_y
Cuts at: 0 393 632 2101 2178
Size: 393 239 1469 77
Fragments arranged by size:
1469 393 239 77
BstZ17I GTA'TAC
Cuts at: 0 192 2178
Size: 192 1986
Bsu36I CC'TnA_GG
Cuts at: 0 341 2178
Size: 341 1837
BtsI GCAGTG_nn'
Cuts at: 0 179 932 2178
Size: 179 753 1246
Fragments arranged by size:
1246 753 179
ClaI AT'CG_AT
Cuts at: 0 201 2178
Size: 201 1977
EaeI y'GGCC_r
Cuts at: 0 122 2125 2178
Size: 122 2003 53
Fragments arranged by size:
2003 122 53
EarI CTCTTCn'nnn_
Cuts at: 0 876 944 1204 1704 1727 1797 2178
Size: 876 68 260 500 23 70 381
Fragments arranged by size:
876 500 381 260 70 68 23
EcoICRI GAG'CTC
Cuts at: 0 869 2178
Size: 869 1309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 236 818 857 1240 1283 1751 1760
Size: 118 118 582 39 383 43 468 9
Cuts at: 1760 2178
Size: 418
Fragments arranged by size:
582 468 418 383 118 118 43 39
9
EcoO109I rG'GnC_Cy
Cuts at: 0 1723 1924 2178
Size: 1723 201 254
Fragments arranged by size:
1723 254 201
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 389 2178
Size: 389 1789
FspI TGC'GCA
Cuts at: 0 1447 2178
Size: 1447 731
FspAI rTGC'GCAy
Cuts at: 0 1447 2178
Size: 1447 731
HincII GTy'rAC
Cuts at: 0 675 908 2178
Size: 675 233 1270
Fragments arranged by size:
1270 675 233
MfeI C'AATT_G
Cuts at: 0 166 446 624 2178
Size: 166 280 178 1554
Fragments arranged by size:
1554 280 178 166
MslI CAynn'nnrTG
Cuts at: 0 464 2178
Size: 464 1714
MspA1I CmG'CkG
Cuts at: 0 54 1468 1886 2178
Size: 54 1414 418 292
Fragments arranged by size:
1414 418 292 54
NdeI CA'TA_TG
Cuts at: 0 1257 2178
Size: 1257 921
NsiI A_TGCA'T
Cuts at: 0 200 1113 1257 2178
Size: 200 913 144 921
Fragments arranged by size:
921 913 200 144
NspI r_CATG'y
Cuts at: 0 198 310 782 906 1189 2178
Size: 198 112 472 124 283 989
Fragments arranged by size:
989 472 283 198 124 112
PasI CC'CwG_GG
Cuts at: 0 2010 2178
Size: 2010 168
PciI A'CATG_T
Cuts at: 0 306 2178
Size: 306 1872
PflMI CCAn_nnn'nTGG
Cuts at: 0 1099 2178
Size: 1099 1079
PpuMI rG'GwC_Cy
Cuts at: 0 1723 2178
Size: 1723 455
PspOMI G'GGCC_C
Cuts at: 0 1924 2178
Size: 1924 254
PstI C_TGCA'G
Cuts at: 0 569 1467 2178
Size: 569 898 711
Fragments arranged by size:
898 711 569
PvuII CAG'CTG
Cuts at: 0 54 1468 1886 2178
Size: 54 1414 418 292
Fragments arranged by size:
1414 418 292 54
SacI G_AGCT'C
Cuts at: 0 871 2178
Size: 871 1307
SanDI GG'GwC_CC
Cuts at: 0 1723 2178
Size: 1723 455
SapI GCTCTTCn'nnn_
Cuts at: 0 876 2178
Size: 876 1302
ScaI AGT'ACT
Cuts at: 0 1782 2178
Size: 1782 396
SfcI C'TryA_G
Cuts at: 0 185 565 724 1463 1484 1674 2178
Size: 185 380 159 739 21 190 504
Fragments arranged by size:
739 504 380 190 185 159 21
SmlI C'TyrA_G
Cuts at: 0 44 2097 2178
Size: 44 2053 81
Fragments arranged by size:
2053 81 44
SpeI A'CTAG_T
Cuts at: 0 1051 2178
Size: 1051 1127
SphI G_CATG'C
Cuts at: 0 782 1189 2178
Size: 782 407 989
Fragments arranged by size:
989 782 407
StyI C'CwwG_G
Cuts at: 0 689 2178
Size: 689 1489
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 470 827 1553 2069 2178
Size: 470 357 726 516 109
Fragments arranged by size:
726 516 470 357 109
TatI w'GTAC_w
Cuts at: 0 569 1780 2178
Size: 569 1211 398
Fragments arranged by size:
1211 569 398
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 230 262 1573 1605 1733 1765 2178
Size: 230 32 1311 32 128 32 413
Fragments arranged by size:
1311 413 230 128 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 687 1600 2178
Size: 687 913 578
Fragments arranged by size:
913 687 578
XhoI C'TCGA_G
Cuts at: 0 44 2097 2178
Size: 44 2053 81
Fragments arranged by size:
2053 81 44
XmnI GAAnn'nnTTC
Cuts at: 0 350 2048 2178
Size: 350 1698 130
Fragments arranged by size:
1698 350 130
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI ApaI ApaLI ApoI
AvaI BanII BbsI BclI BfrBI BglII Bme1580I BmrI
BpmI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I BtsI ClaI EaeI
EarI EcoICRI Eco57MI EcoO109I FalI FspI FspAI HincII
MfeI MslI MspA1I NdeI NsiI NspI PasI PciI
PflMI PpuMI PspOMI PstI PvuII SacI SanDI SapI
ScaI SfcI SmlI SpeI SphI StyI TaqII TatI
TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI AlwNI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BciVI BglI BlpI BmgBI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI
BsiEI BsiWI BspEI BsrFI BsrGI BssHII BstEII BtgI
BtgZI CspCI DraI DraIII DrdI EagI EciI EcoNI
EcoRI EcoRV FseI HaeII Hin4I HindIII HpaI KasI
KpnI MluI MmeI MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI PacI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SrfI SspI StuI SwaI Tth111I XbaI XmaI ZraI