(Linear) (Six-base) MAPSORT of: KIEE2258.seq  Check: 275  from: 1  to: 351



KIEE2258.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 17:18  ..

AccI GT'mk_AC

Cuts at:      0     341     351 
   Size:        341      10
 
ApoI r'AATT_y

Cuts at:      0     200     351 
   Size:        200     151
 
BanI G'GyrC_C

Cuts at:      0      84     150     351 
   Size:         84      66     201

  Fragments arranged by size: 

                 201      84      66
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     308     351 
   Size:        308      43
 
BbvCI CC'TCA_GC

Cuts at:      0     215     351 
   Size:        215     136
 
Bpu10I CC'TnA_GC

Cuts at:      0     215     351 
   Size:        215     136
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     119     351 
   Size:        119     232
 
BsaWI w'CCGG_w

Cuts at:      0     191     351 
   Size:        191     160
 
BspEI T'CCGG_A

Cuts at:      0     191     351 
   Size:        191     160
 
BsrBI CCG'CTC

Cuts at:      0      44     351 
   Size:         44     307
 
BsrDI GCAATG_nn'

Cuts at:      0     317     351 
   Size:        317      34
 
BsrFI r'CCGG_y

Cuts at:      0      87     351 
   Size:         87     264
 
BsrGI T'GTAC_A

Cuts at:      0      11     351 
   Size:         11     340
 
BstYI r'GATC_y

Cuts at:      0      59     351 
   Size:         59     292
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     337     351 
   Size:        337      14
 
EarI CTCTTCn'nnn_

Cuts at:      0      14     351 
   Size:         14     337
 
EcoRI G'AATT_C

Cuts at:      0     200     351 
   Size:        200     151
 
HaeII r_GCGC'y

Cuts at:      0      42     351 
   Size:         42     309
 
HincII GTy'rAC

Cuts at:      0     240     351 
   Size:        240     111
 
MspA1I CmG'CkG

Cuts at:      0     279     351 
   Size:        279      72
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      13      45     351 
   Size:         13      32     306

  Fragments arranged by size: 

                 306      32      13
 
PvuII CAG'CTG

Cuts at:      0     279     351 
   Size:        279      72
 
RsrII CG'GwC_CG

Cuts at:      0      78     351 
   Size:         78     273
 
SapI GCTCTTCn'nnn_

Cuts at:      0      14     351 
   Size:         14     337
 
SexAI A'CCwGG_T

Cuts at:      0     146     351 
   Size:        146     205
 
SfcI C'TryA_G

Cuts at:      0     324     351 
   Size:        324      27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     270     351 
   Size:        270      81
 
TatI w'GTAC_w

Cuts at:      0      11     351 
   Size:         11     340
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     267     351 
   Size:        267      84
 

 Enzymes that do cut:

     AccI     ApoI     BanI     BbsI    BbvCI   Bpu10I     BsaI    BsaWI
    BspEI    BsrBI    BsrDI    BsrFI    BsrGI    BstYI     DrdI     EarI
    EcoRI    HaeII   HincII   MspA1I     PpiI    PvuII    RsrII     SapI
    SexAI     SfcI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI    BsaAI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspHI    BspMI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI     SacI    SacII     SalI    SanDI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI