Restriction map for
pFN21AE2246
(Linear) (Six-base) MAPSORT of: KIEE2246.seq Check: 8345 from: 1 to: 900
KIEE2246.orf
With 182 enzymes: SgfI *
July 15, 2010 18:56 ..
Acc65I G'GTAC_C
Cuts at: 0 612 900
Size: 612 288
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 900
Size: 784 116
AfeI AGC'GCT
Cuts at: 0 259 883 900
Size: 259 624 17
Fragments arranged by size:
624 259 17
AflIII A'CryG_T
Cuts at: 0 205 900
Size: 205 695
AleI CACnn'nnGTG
Cuts at: 0 169 329 900
Size: 169 160 571
Fragments arranged by size:
571 169 160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 187 327 900
Size: 187 140 573
Fragments arranged by size:
573 187 140
AlwNI CAG_nnn'CTG
Cuts at: 0 622 693 837 900
Size: 622 71 144 63
Fragments arranged by size:
622 144 71 63
ApaLI G'TGCA_C
Cuts at: 0 576 895 900
Size: 576 319 5
Fragments arranged by size:
576 319 5
AvaI C'yCGr_G
Cuts at: 0 420 706 856 900
Size: 420 286 150 44
Fragments arranged by size:
420 286 150 44
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 596 629 900
Size: 596 33 271
Fragments arranged by size:
596 271 33
BanI G'GyrC_C
Cuts at: 0 69 163 403 424 466 612 900
Size: 69 94 240 21 42 146 288
Fragments arranged by size:
288 240 146 94 69 42 21
BanII G_rGCy'C
Cuts at: 0 791 900
Size: 791 109
BbvCI CC'TCA_GC
Cuts at: 0 601 623 900
Size: 601 22 277
Fragments arranged by size:
601 277 22
BglI GCCn_nnn'nGGC
Cuts at: 0 161 350 464 709 900
Size: 161 189 114 245 191
Fragments arranged by size:
245 191 189 161 114
Bme1580I G_kGCm'C
Cuts at: 0 157 427 460 580 899 900
Size: 157 270 33 120 319 1
Fragments arranged by size:
319 270 157 120 33 1
BmgBI CAC'GTC
Cuts at: 0 206 900
Size: 206 694
BmrI ACTGGGnnnn_n'
Cuts at: 0 144 900
Size: 144 756
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 274 471 883 900
Size: 31 243 197 412 17
Fragments arranged by size:
412 243 197 31 17
Bpu10I CC'TnA_GC
Cuts at: 0 601 623 900
Size: 601 22 277
Fragments arranged by size:
601 277 22
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 861 891 900
Size: 861 30 9
Fragments arranged by size:
861 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 310 313 640 900
Size: 310 3 327 260
Fragments arranged by size:
327 310 260 3
BseYI C'CCAG_C
Cuts at: 0 430 722 900
Size: 430 292 178
Fragments arranged by size:
430 292 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 504 570 900
Size: 193 311 66 330
Fragments arranged by size:
330 311 193 66
BsiEI CG_ry'CG
Cuts at: 0 181 900
Size: 181 719
BsiHKAI G_wGCw'C
Cuts at: 0 386 580 791 899 900
Size: 386 194 211 108 1
Fragments arranged by size:
386 211 194 108 1
Bsp1286I G_dGCh'C
Cuts at: 0 157 386 427 460 580 791 899 900
Size: 157 229 41 33 120 211 108 1
Fragments arranged by size:
229 211 157 120 108 41 33 1
BsrBI CCG'CTC
Cuts at: 0 135 867 900
Size: 135 732 33
Fragments arranged by size:
732 135 33
BsrFI r'CCGG_y
Cuts at: 0 66 72 349 406 900
Size: 66 6 277 57 494
Fragments arranged by size:
494 277 66 57 6
BstAPI GCAn_nnn'nTGC
Cuts at: 0 584 693 837 900
Size: 584 109 144 63
Fragments arranged by size:
584 144 109 63
BtsI GCAGTG_nn'
Cuts at: 0 451 505 900
Size: 451 54 395
Fragments arranged by size:
451 395 54
DraIII CAC_nnn'GTG
Cuts at: 0 206 403 446 575 900
Size: 206 197 43 129 325
Fragments arranged by size:
325 206 197 129 43
EaeI y'GGCC_r
Cuts at: 0 178 845 900
Size: 178 667 55
Fragments arranged by size:
667 178 55
EagI C'GGCC_G
Cuts at: 0 178 900
Size: 178 722
EarI CTCTTCn'nnn_
Cuts at: 0 561 900
Size: 561 339
EcoICRI GAG'CTC
Cuts at: 0 789 900
Size: 789 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 274 471 784 883 900
Size: 31 243 197 313 99 17
Fragments arranged by size:
313 243 197 99 31 17
EcoO109I rG'GnC_Cy
Cuts at: 0 702 711 900
Size: 702 9 189
Fragments arranged by size:
702 189 9
HaeII r_GCGC'y
Cuts at: 0 10 52 261 814 838 885 900
Size: 10 42 209 553 24 47 15
Fragments arranged by size:
553 209 47 42 24 15 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 373 405 900
Size: 373 32 495
Fragments arranged by size:
495 373 32
KpnI G_GTAC'C
Cuts at: 0 616 900
Size: 616 284
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 900
Size: 754 146
MscI TGG'CCA
Cuts at: 0 847 900
Size: 847 53
MslI CAynn'nnrTG
Cuts at: 0 169 329 900
Size: 169 160 571
Fragments arranged by size:
571 169 160
MspA1I CmG'CkG
Cuts at: 0 177 341 584 690 762 900
Size: 177 164 243 106 72 138
Fragments arranged by size:
243 177 164 138 106 72
NaeI GCC'GGC
Cuts at: 0 74 408 900
Size: 74 334 492
Fragments arranged by size:
492 334 74
NgoMIV G'CCGG_C
Cuts at: 0 72 406 900
Size: 72 334 494
Fragments arranged by size:
494 334 72
PasI CC'CwG_GG
Cuts at: 0 528 900
Size: 528 372
PfoI T'CCnGG_A
Cuts at: 0 662 900
Size: 662 238
PspXI vC'TCGA_Gb
Cuts at: 0 706 900
Size: 706 194
PstI C_TGCA'G
Cuts at: 0 689 701 833 900
Size: 689 12 132 67
Fragments arranged by size:
689 132 67 12
PvuII CAG'CTG
Cuts at: 0 584 762 900
Size: 584 178 138
Fragments arranged by size:
584 178 138
SacI G_AGCT'C
Cuts at: 0 791 900
Size: 791 109
SapI GCTCTTCn'nnn_
Cuts at: 0 561 900
Size: 561 339
SexAI A'CCwGG_T
Cuts at: 0 399 900
Size: 399 501
SfcI C'TryA_G
Cuts at: 0 685 697 829 900
Size: 685 12 132 71
Fragments arranged by size:
685 132 71 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 709 900
Size: 709 191
SmaI CCC'GGG
Cuts at: 0 422 858 900
Size: 422 436 42
Fragments arranged by size:
436 422 42
SmlI C'TyrA_G
Cuts at: 0 706 900
Size: 706 194
SrfI GCCC'GGGC
Cuts at: 0 858 900
Size: 858 42
StuI AGG'CCT
Cuts at: 0 23 900
Size: 23 877
StyI C'CwwG_G
Cuts at: 0 736 900
Size: 736 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 114 337 900
Size: 114 223 563
Fragments arranged by size:
563 223 114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 861 893 900
Size: 861 32 7
Fragments arranged by size:
861 32 7
XhoI C'TCGA_G
Cuts at: 0 706 900
Size: 706 194
XmaI C'CCGG_G
Cuts at: 0 420 856 900
Size: 420 436 44
Fragments arranged by size:
436 420 44
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AleI AlfI AlwNI ApaLI
AvaI BaeI BanI BanII BbvCI BglI Bme1580I BmgBI
BmrI BpmI Bpu10I BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BsrBI BsrFI BstAPI BtsI DraIII EaeI
EagI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I KpnI
MmeI MscI MslI MspA1I NaeI NgoMIV PasI PfoI
PspXI PstI PvuII SacI SapI SexAI SfcI SfiI
SmaI SmlI SrfI StuI StyI TaqII TstI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI AloI
ApaI ApoI AscI AseI AsiSI AvrII BamHI BbeI
BbsI BcgI BciVI BclI BfrBI BglII BlpI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DrdI EciI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI MfeI MluI NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SfoI SgfI SgrAI
SnaBI SpeI SphI SspI SwaI TatI Tth111I XbaI
XcmI XmnI ZraI