(Linear) (Six-base) MAPSORT of: KIEE2245.seq  Check: 2179  from: 1  to: 651



KIEE2245.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:56  ..

AleI CACnn'nnGTG

Cuts at:      0      64     651 
   Size:         64     587
 
ApoI r'AATT_y

Cuts at:      0     116     400     498     567     651 
   Size:        116     284      98      69      84

  Fragments arranged by size: 

                 284     116      98      84      69
 
AvrII C'CTAG_G

Cuts at:      0     222     651 
   Size:        222     429
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     246     651 
   Size:        246     405
 
Bpu10I CC'TnA_GC

Cuts at:      0     178     651 
   Size:        178     473
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     651 
   Size:        190     461
 
BsmI GAATG_Cn'

Cuts at:      0      28     651 
   Size:         28     623
 
BspHI T'CATG_A

Cuts at:      0     243     651 
   Size:        243     408
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     197     651 
   Size:        197     454
 
BsrDI GCAATG_nn'

Cuts at:      0      61     651 
   Size:         61     590
 
BstYI r'GATC_y

Cuts at:      0     354     651 
   Size:        354     297
 
BtgI C'CryG_G

Cuts at:      0     211     651 
   Size:        211     440
 
BtsI GCAGTG_nn'

Cuts at:      0     153     651 
   Size:        153     498
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      13     651 
   Size:         13     638
 
EcoO109I rG'GnC_Cy

Cuts at:      0     174     651 
   Size:        174     477
 
EcoRI G'AATT_C

Cuts at:      0     116     498     651 
   Size:        116     382     153

  Fragments arranged by size: 

                 382     153     116
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     166     198     651 
   Size:        166      32     453

  Fragments arranged by size: 

                 453     166      32
 
MfeI C'AATT_G

Cuts at:      0     476     651 
   Size:        476     175
 
MslI CAynn'nnrTG

Cuts at:      0      64     449     651 
   Size:         64     385     202

  Fragments arranged by size: 

                 385     202      64
 
NcoI C'CATG_G

Cuts at:      0     211     651 
   Size:        211     440
 
NdeI CA'TA_TG

Cuts at:      0     297     559     651 
   Size:        297     262      92

  Fragments arranged by size: 

                 297     262      92
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     571     651 
   Size:        571      80
 
PfoI T'CCnGG_A

Cuts at:      0      34     651 
   Size:         34     617
 
PpuMI rG'GwC_Cy

Cuts at:      0     174     651 
   Size:        174     477
 
StyI C'CwwG_G

Cuts at:      0     211     222     651 
   Size:        211      11     429

  Fragments arranged by size: 

                 429     211      11
 
TatI w'GTAC_w

Cuts at:      0       2     651 
   Size:          2     649
 

 Enzymes that do cut:

     AleI     ApoI    AvrII    BciVI   Bpu10I     BsgI     BsmI    BspHI
    BspMI    BsrDI    BstYI     BtgI     BtsI    EcoNI EcoO109I    EcoRI
    Hin4I     MfeI     MslI     NcoI     NdeI    PflMI     PfoI    PpuMI
     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI