Restriction map for
pFN21AE2242
(Linear) (Six-base) MAPSORT of: KIEE2242.seq Check: 4086 from: 1 to: 1041
KIEE2242.orf
With 182 enzymes: SgfI *
July 3, 2012 17:18 ..
Acc65I G'GTAC_C
Cuts at: 0 895 1041
Size: 895 146
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1041
Size: 799 242
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 469 501 1041
Size: 469 32 540
Fragments arranged by size:
540 469 32
AlwNI CAG_nnn'CTG
Cuts at: 0 801 1041
Size: 801 240
ApaI G_GGCC'C
Cuts at: 0 731 1041
Size: 731 310
ApaLI G'TGCA_C
Cuts at: 0 657 1041
Size: 657 384
AvaI C'yCGr_G
Cuts at: 0 724 1041
Size: 724 317
AvrII C'CTAG_G
Cuts at: 0 969 1041
Size: 969 72
BamHI G'GATC_C
Cuts at: 0 540 1041
Size: 540 501
BanI G'GyrC_C
Cuts at: 0 895 1005 1041
Size: 895 110 36
Fragments arranged by size:
895 110 36
BanII G_rGCy'C
Cuts at: 0 731 760 1041
Size: 731 29 281
Fragments arranged by size:
731 281 29
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 324 358 1041
Size: 324 34 683
Fragments arranged by size:
683 324 34
BclI T'GATC_A
Cuts at: 0 803 1041
Size: 803 238
Bme1580I G_kGCm'C
Cuts at: 0 661 731 1041
Size: 661 70 310
Fragments arranged by size:
661 310 70
BmrI ACTGGGnnnn_n'
Cuts at: 0 575 1041
Size: 575 466
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 1041
Size: 325 716
Bpu10I CC'TnA_GC
Cuts at: 0 59 1041
Size: 59 982
BsaI GGTCTCn'nnnn_
Cuts at: 0 574 1041
Size: 574 467
BsaHI Gr'CG_yC
Cuts at: 0 1015 1041
Size: 1015 26
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1 31 1041
Size: 1 30 1010
Fragments arranged by size:
1010 30 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 610 819 849 1041
Size: 610 209 30 192
Fragments arranged by size:
610 209 192 30
BseYI C'CCAG_C
Cuts at: 0 1018 1041
Size: 1018 23
BsiHKAI G_wGCw'C
Cuts at: 0 661 760 1041
Size: 661 99 281
Fragments arranged by size:
661 281 99
BsiWI C'GTAC_G
Cuts at: 0 708 1041
Size: 708 333
Bsp1286I G_dGCh'C
Cuts at: 0 661 731 760 1041
Size: 661 70 29 281
Fragments arranged by size:
661 281 70 29
BsrDI GCAATG_nn'
Cuts at: 0 275 628 1041
Size: 275 353 413
Fragments arranged by size:
413 353 275
BsrFI r'CCGG_y
Cuts at: 0 809 1002 1041
Size: 809 193 39
Fragments arranged by size:
809 193 39
BstYI r'GATC_y
Cuts at: 0 496 540 1041
Size: 496 44 501
Fragments arranged by size:
501 496 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 39 1041
Size: 39 1002
EarI CTCTTCn'nnn_
Cuts at: 0 232 454 1041
Size: 232 222 587
Fragments arranged by size:
587 232 222
EcoICRI GAG'CTC
Cuts at: 0 758 1041
Size: 758 283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 799 1041
Size: 325 474 242
Fragments arranged by size:
474 325 242
EcoNI CCTnn'n_nnAGG
Cuts at: 0 82 745 1041
Size: 82 663 296
Fragments arranged by size:
663 296 82
EcoO109I rG'GnC_Cy
Cuts at: 0 665 728 798 1041
Size: 665 63 70 243
Fragments arranged by size:
665 243 70 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 814 841 846 861 873 893
Size: 1 32 781 27 5 15 12 20
Cuts at: 893 1041
Size: 148
Fragments arranged by size:
781 148 32 27 20 15 12 5
1
KpnI G_GTAC'C
Cuts at: 0 899 1041
Size: 899 142
MfeI C'AATT_G
Cuts at: 0 261 1041
Size: 261 780
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 1001 1041
Size: 307 694 40
Fragments arranged by size:
694 307 40
MslI CAynn'nnrTG
Cuts at: 0 518 689 1041
Size: 518 171 352
Fragments arranged by size:
518 352 171
NaeI GCC'GGC
Cuts at: 0 811 1041
Size: 811 230
NgoMIV G'CCGG_C
Cuts at: 0 809 1041
Size: 809 232
PasI CC'CwG_GG
Cuts at: 0 585 1041
Size: 585 456
PpuMI rG'GwC_Cy
Cuts at: 0 665 1041
Size: 665 376
PspOMI G'GGCC_C
Cuts at: 0 727 1041
Size: 727 314
SacI G_AGCT'C
Cuts at: 0 760 1041
Size: 760 281
SapI GCTCTTCn'nnn_
Cuts at: 0 232 1041
Size: 232 809
SexAI A'CCwGG_T
Cuts at: 0 77 1041
Size: 77 964
StyI C'CwwG_G
Cuts at: 0 969 1041
Size: 969 72
Tth111I GACn'n_nGTC
Cuts at: 0 79 1041
Size: 79 962
Enzymes that do cut:
Acc65I AcuI AloI AlwNI ApaI ApaLI AvaI AvrII
BamHI BanI BanII BcgI BclI Bme1580I BmrI BpmI
Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsiHKAI BsiWI
Bsp1286I BsrDI BsrFI BstYI BtgZI EarI EcoICRI Eco57MI
EcoNI EcoO109I Hin4I KpnI MfeI MmeI MslI NaeI
NgoMIV PasI PpuMI PspOMI SacI SapI SexAI StyI
Tth111I
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApoI AscI AseI AsiSI BaeI
BbeI BbsI BbvCI BciVI BfrBI BglI BglII BlpI
BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaWI BsgI
BsiEI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI MluI MscI MspA1I
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI XbaI XcmI XhoI
XmaI XmnI ZraI