Restriction map for
pFN21AE2241
(Linear) (Six-base) MAPSORT of: KIEE2241.seq Check: 2715 from: 1 to: 1527
KIEE2241.orf
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 243 1006 1527
Size: 243 763 521
Fragments arranged by size:
763 521 243
AatII G_ACGT'C
Cuts at: 0 215 1527
Size: 215 1312
AccI GT'mk_AC
Cuts at: 0 200 1527
Size: 200 1327
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 484 1527
Size: 256 228 1043
Fragments arranged by size:
1043 256 228
AflII C'TTAA_G
Cuts at: 0 121 1527
Size: 121 1406
AflIII A'CryG_T
Cuts at: 0 161 275 1527
Size: 161 114 1252
Fragments arranged by size:
1252 161 114
AhdI GACnn_n'nnGTC
Cuts at: 0 330 1527
Size: 330 1197
AlwNI CAG_nnn'CTG
Cuts at: 0 998 1527
Size: 998 529
ApaI G_GGCC'C
Cuts at: 0 272 1527
Size: 272 1255
ApoI r'AATT_y
Cuts at: 0 980 1527
Size: 980 547
AvaI C'yCGr_G
Cuts at: 0 340 1527
Size: 340 1187
BamHI G'GATC_C
Cuts at: 0 689 1527
Size: 689 838
BanI G'GyrC_C
Cuts at: 0 93 1527
Size: 93 1434
BanII G_rGCy'C
Cuts at: 0 272 632 653 1441 1504 1527
Size: 272 360 21 788 63 23
Fragments arranged by size:
788 360 272 63 23 21
BclI T'GATC_A
Cuts at: 0 758 1527
Size: 758 769
BglI GCCn_nnn'nGGC
Cuts at: 0 1462 1527
Size: 1462 65
BlpI GC'TnA_GC
Cuts at: 0 626 718 1168 1527
Size: 626 92 450 359
Fragments arranged by size:
626 450 359 92
Bme1580I G_kGCm'C
Cuts at: 0 272 1014 1527
Size: 272 742 513
Fragments arranged by size:
742 513 272
BmrI ACTGGGnnnn_n'
Cuts at: 0 137 1217 1326 1527
Size: 137 1080 109 201
Fragments arranged by size:
1080 201 137 109
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1119 1527
Size: 1119 408
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 991 1527
Size: 835 156 536
Fragments arranged by size:
835 536 156
BsaAI yAC'GTr
Cuts at: 0 276 1527
Size: 276 1251
BsaBI GATnn'nnATC
Cuts at: 0 178 1527
Size: 178 1349
BsaHI Gr'CG_yC
Cuts at: 0 44 212 1527
Size: 44 168 1315
Fragments arranged by size:
1315 168 44
BsaWI w'CCGG_w
Cuts at: 0 1391 1527
Size: 1391 136
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1413 1443 1476 1506 1527
Size: 1413 30 33 30 21
Fragments arranged by size:
1413 33 30 30 21
BseYI C'CCAG_C
Cuts at: 0 23 39 69 223 1350 1527
Size: 23 16 30 154 1127 177
Fragments arranged by size:
1127 177 154 30 23 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1066 1527
Size: 1066 461
BsiHKAI G_wGCw'C
Cuts at: 0 632 1527
Size: 632 895
BsmI GAATG_Cn'
Cuts at: 0 484 738 1527
Size: 484 254 789
Fragments arranged by size:
789 484 254
Bsp1286I G_dGCh'C
Cuts at: 0 272 632 653 1014 1441 1504 1527
Size: 272 360 21 361 427 63 23
Fragments arranged by size:
427 361 360 272 63 23 21
BspEI T'CCGG_A
Cuts at: 0 1391 1527
Size: 1391 136
BspHI T'CATG_A
Cuts at: 0 80 215 473 512 794 1527
Size: 80 135 258 39 282 733
Fragments arranged by size:
733 282 258 135 80 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 105 243 992 1006 1527
Size: 105 138 749 14 521
Fragments arranged by size:
749 521 138 105 14
BsrBI CCG'CTC
Cuts at: 0 1256 1527
Size: 1256 271
BsrGI T'GTAC_A
Cuts at: 0 278 857 1527
Size: 278 579 670
Fragments arranged by size:
670 579 278
BssHII G'CGCG_C
Cuts at: 0 19 1527
Size: 19 1508
BssSI C'ACGA_G
Cuts at: 0 697 1527
Size: 697 830
BstXI CCAn_nnnn'nTGG
Cuts at: 0 417 1527
Size: 417 1110
BstYI r'GATC_y
Cuts at: 0 173 230 689 1527
Size: 173 57 459 838
Fragments arranged by size:
838 459 173 57
BtgI C'CryG_G
Cuts at: 0 378 1262 1527
Size: 378 884 265
Fragments arranged by size:
884 378 265
DraIII CAC_nnn'GTG
Cuts at: 0 699 1527
Size: 699 828
DrdI GACnn_nn'nnGTC
Cuts at: 0 400 804 1527
Size: 400 404 723
Fragments arranged by size:
723 404 400
EaeI y'GGCC_r
Cuts at: 0 784 1527
Size: 784 743
EarI CTCTTCn'nnn_
Cuts at: 0 658 1283 1527
Size: 658 625 244
Fragments arranged by size:
658 625 244
EcoICRI GAG'CTC
Cuts at: 0 630 1527
Size: 630 897
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 484 835 991 1527
Size: 256 228 351 156 536
Fragments arranged by size:
536 351 256 228 156
EcoO109I rG'GnC_Cy
Cuts at: 0 117 269 552 1527
Size: 117 152 283 975
Fragments arranged by size:
975 283 152 117
EcoRI G'AATT_C
Cuts at: 0 980 1527
Size: 980 547
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 747 1527
Size: 747 780
FspI TGC'GCA
Cuts at: 0 133 1527
Size: 133 1394
FspAI rTGC'GCAy
Cuts at: 0 133 1527
Size: 133 1394
HaeII r_GCGC'y
Cuts at: 0 40 77 1351 1527
Size: 40 37 1274 176
Fragments arranged by size:
1274 176 40 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 316 348 1476 1508 1527
Size: 316 32 1128 32 19
Fragments arranged by size:
1128 316 32 32 19
HincII GTy'rAC
Cuts at: 0 729 1527
Size: 729 798
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 1527
Size: 665 862
MslI CAynn'nnrTG
Cuts at: 0 85 403 1527
Size: 85 318 1124
Fragments arranged by size:
1124 318 85
MspA1I CmG'CkG
Cuts at: 0 1167 1236 1299 1527
Size: 1167 69 63 228
Fragments arranged by size:
1167 228 69 63
NcoI C'CATG_G
Cuts at: 0 1262 1527
Size: 1262 265
NspI r_CATG'y
Cuts at: 0 165 594 1527
Size: 165 429 933
Fragments arranged by size:
933 429 165
PciI A'CATG_T
Cuts at: 0 161 1527
Size: 161 1366
PflMI CCAn_nnn'nTGG
Cuts at: 0 699 1527
Size: 699 828
PfoI T'CCnGG_A
Cuts at: 0 1112 1527
Size: 1112 415
PpuMI rG'GwC_Cy
Cuts at: 0 117 552 1527
Size: 117 435 975
Fragments arranged by size:
975 435 117
PspOMI G'GGCC_C
Cuts at: 0 268 1527
Size: 268 1259
PspXI vC'TCGA_Gb
Cuts at: 0 340 1527
Size: 340 1187
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 271 303 1527
Size: 271 32 1224
Fragments arranged by size:
1224 271 32
PstI C_TGCA'G
Cuts at: 0 1003 1166 1514 1527
Size: 1003 163 348 13
Fragments arranged by size:
1003 348 163 13
PvuII CAG'CTG
Cuts at: 0 1167 1299 1527
Size: 1167 132 228
Fragments arranged by size:
1167 228 132
SacI G_AGCT'C
Cuts at: 0 632 1527
Size: 632 895
SapI GCTCTTCn'nnn_
Cuts at: 0 658 1527
Size: 658 869
SbfI CC_TGCA'GG
Cuts at: 0 1003 1527
Size: 1003 524
ScaI AGT'ACT
Cuts at: 0 580 1527
Size: 580 947
SfcI C'TryA_G
Cuts at: 0 822 999 1162 1510 1527
Size: 822 177 163 348 17
Fragments arranged by size:
822 348 177 163 17
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1462 1527
Size: 1462 65
SmlI C'TyrA_G
Cuts at: 0 121 340 1527
Size: 121 219 1187
Fragments arranged by size:
1187 219 121
StuI AGG'CCT
Cuts at: 0 835 1465 1527
Size: 835 630 62
Fragments arranged by size:
835 630 62
StyI C'CwwG_G
Cuts at: 0 479 1262 1313 1496 1527
Size: 479 783 51 183 31
Fragments arranged by size:
783 479 183 51 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 415 517 960 1527
Size: 415 102 443 567
Fragments arranged by size:
567 443 415 102
TatI w'GTAC_w
Cuts at: 0 278 578 857 1527
Size: 278 300 279 670
Fragments arranged by size:
670 300 279 278
XhoI C'TCGA_G
Cuts at: 0 340 1527
Size: 340 1187
ZraI GAC'GTC
Cuts at: 0 213 1527
Size: 213 1314
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AhdI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BclI BglI
BlpI Bme1580I BmrI BplI BpmI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrGI BssHII BssSI BstXI BstYI
BtgI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII MmeI
MslI MspA1I NcoI NspI PciI PflMI PfoI PpuMI
PspOMI PspXI PsrI PstI PvuII SacI SapI SbfI
ScaI SfcI SfiI SmlI StuI StyI TaqII TatI
XhoI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AleI AlfI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BbsI BbvCI
BcgI BciVI BfrBI BglII BmgBI BmtI Bpu10I BpuEI
BsaI BseRI BsiEI BsiWI BsmBI BsrDI BsrFI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI EagI EciI EcoNI EcoRV FseI HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PmeI
PmlI PpiI PshAI PsiI PvuI RsrII SacII SalI
SanDI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TstI Tth111I XbaI XcmI
XmaI XmnI