(Linear) (Six-base) MAPSORT of: KIEE2240.seq  Check: 9969  from: 1  to: 240



KIEE2240.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 17:17  ..

AleI CACnn'nnGTG

Cuts at:      0     166     240 
   Size:        166      74
 
AvaI C'yCGr_G

Cuts at:      0      25     240 
   Size:         25     215
 
BanI G'GyrC_C

Cuts at:      0     178     240 
   Size:        178      62
 
Bme1580I G_kGCm'C

Cuts at:      0     183     240 
   Size:        183      57
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     216     240 
   Size:        216      24
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      99     240 
   Size:         99     141
 
BseYI C'CCAG_C

Cuts at:      0      30     240 
   Size:         30     210
 
Bsp1286I G_dGCh'C

Cuts at:      0     183     240 
   Size:        183      57
 
BsrDI GCAATG_nn'

Cuts at:      0      17     240 
   Size:         17     223
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     168     240 
   Size:        168      72
 
BtsI GCAGTG_nn'

Cuts at:      0      49     240 
   Size:         49     191
 
EaeI y'GGCC_r

Cuts at:      0      17     240 
   Size:         17     223
 
EarI CTCTTCn'nnn_

Cuts at:      0     208     227     240 
   Size:        208      19      13

  Fragments arranged by size: 

                 208      19      13
 
HincII GTy'rAC

Cuts at:      0     191     240 
   Size:        191      49
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     172     240 
   Size:        149      23      68

  Fragments arranged by size: 

                 149      68      23
 
MscI TGG'CCA

Cuts at:      0      19     240 
   Size:         19     221
 
MslI CAynn'nnrTG

Cuts at:      0     166     240 
   Size:        166      74
 
PstI C_TGCA'G

Cuts at:      0     188     240 
   Size:        188      52
 
ScaI AGT'ACT

Cuts at:      0     120     240 
   Size:        120     120
 
SfcI C'TryA_G

Cuts at:      0     184     240 
   Size:        184      56
 
StyI C'CwwG_G

Cuts at:      0     164     240 
   Size:        164      76
 
TatI w'GTAC_w

Cuts at:      0     118     240 
   Size:        118     122
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      27     132     165     204     240 
   Size:         27     105      33      39      36

  Fragments arranged by size: 

                 105      39      36      33      27
 

 Enzymes that do cut:

     AleI     AvaI     BanI Bme1580I     BsaI    BseRI    BseYI Bsp1286I
    BsrDI    BstXI     BtsI     EaeI     EarI   HincII     MmeI     MscI
     MslI     PstI     ScaI     SfcI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI