Restriction map for
pFN21AE2236
(Linear) (Six-base) MAPSORT of: KIEE2236.seq Check: 1308 from: 1 to: 1428
KIEE2236.orf
With 182 enzymes: SgfI *
July 3, 2012 17:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 713 1329 1428
Size: 279 434 616 99
Fragments arranged by size:
616 434 279 99
ApoI r'AATT_y
Cuts at: 0 970 1428
Size: 970 458
BanI G'GyrC_C
Cuts at: 0 622 819 1428
Size: 622 197 609
Fragments arranged by size:
622 609 197
BanII G_rGCy'C
Cuts at: 0 674 734 1428
Size: 674 60 694
Fragments arranged by size:
694 674 60
BfrBI ATG'CAT
Cuts at: 0 6 616 1428
Size: 6 610 812
Fragments arranged by size:
812 610 6
Bme1580I G_kGCm'C
Cuts at: 0 824 1428
Size: 824 604
BmrI ACTGGGnnnn_n'
Cuts at: 0 732 1428
Size: 732 696
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 99 1428
Size: 99 1329
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 429 1242 1428
Size: 268 161 813 186
Fragments arranged by size:
813 268 186 161
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 283 313 1428
Size: 283 30 1115
Fragments arranged by size:
1115 283 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 1428
Size: 91 1337
BseYI C'CCAG_C
Cuts at: 0 101 1428
Size: 101 1327
BsiHKAI G_wGCw'C
Cuts at: 0 734 1428
Size: 734 694
BsmI GAATG_Cn'
Cuts at: 0 13 200 770 1428
Size: 13 187 570 658
Fragments arranged by size:
658 570 187 13
Bsp1286I G_dGCh'C
Cuts at: 0 674 734 824 1428
Size: 674 60 90 604
Fragments arranged by size:
674 604 90 60
BspHI T'CATG_A
Cuts at: 0 648 1428
Size: 648 780
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1096 1428
Size: 1096 332
BsrDI GCAATG_nn'
Cuts at: 0 1422 1428
Size: 1422 6
BstYI r'GATC_y
Cuts at: 0 93 844 946 1428
Size: 93 751 102 482
Fragments arranged by size:
751 482 102 93
Bsu36I CC'TnA_GG
Cuts at: 0 1301 1428
Size: 1301 127
BtgI C'CryG_G
Cuts at: 0 1247 1428
Size: 1247 181
DraI TTT'AAA
Cuts at: 0 924 1062 1428
Size: 924 138 366
Fragments arranged by size:
924 366 138
EaeI y'GGCC_r
Cuts at: 0 657 1428
Size: 657 771
EarI CTCTTCn'nnn_
Cuts at: 0 416 1007 1428
Size: 416 591 421
Fragments arranged by size:
591 421 416
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 733 1428
Size: 733 695
EcoICRI GAG'CTC
Cuts at: 0 732 1428
Size: 732 696
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 279 429 713 1242 1329 1428
Size: 268 11 150 284 529 87 99
Fragments arranged by size:
529 284 268 150 99 87 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 133 1428
Size: 133 1295
FspI TGC'GCA
Cuts at: 0 259 1428
Size: 259 1169
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 496 528 1428
Size: 496 32 900
Fragments arranged by size:
900 496 32
HincII GTy'rAC
Cuts at: 0 1035 1428
Size: 1035 393
HindIII A'AGCT_T
Cuts at: 0 695 1428
Size: 695 733
HpaI GTT'AAC
Cuts at: 0 1035 1428
Size: 1035 393
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 194 1168 1428
Size: 110 84 974 260
Fragments arranged by size:
974 260 110 84
MscI TGG'CCA
Cuts at: 0 659 1428
Size: 659 769
MslI CAynn'nnrTG
Cuts at: 0 587 1428
Size: 587 841
MspA1I CmG'CkG
Cuts at: 0 1168 1210 1415 1428
Size: 1168 42 205 13
Fragments arranged by size:
1168 205 42 13
NcoI C'CATG_G
Cuts at: 0 1247 1428
Size: 1247 181
NdeI CA'TA_TG
Cuts at: 0 1026 1428
Size: 1026 402
NsiI A_TGCA'T
Cuts at: 0 8 618 1428
Size: 8 610 810
Fragments arranged by size:
810 610 8
NspI r_CATG'y
Cuts at: 0 315 1428
Size: 315 1113
PasI CC'CwG_GG
Cuts at: 0 666 1131 1428
Size: 666 465 297
Fragments arranged by size:
666 465 297
PflMI CCAn_nnn'nTGG
Cuts at: 0 666 1131 1248 1428
Size: 666 465 117 180
Fragments arranged by size:
666 465 180 117
PfoI T'CCnGG_A
Cuts at: 0 1287 1428
Size: 1287 141
PshAI GACnn'nnGTC
Cuts at: 0 287 1428
Size: 287 1141
PvuII CAG'CTG
Cuts at: 0 1168 1415 1428
Size: 1168 247 13
Fragments arranged by size:
1168 247 13
SacI G_AGCT'C
Cuts at: 0 734 1428
Size: 734 694
ScaI AGT'ACT
Cuts at: 0 121 1428
Size: 121 1307
SphI G_CATG'C
Cuts at: 0 315 1428
Size: 315 1113
StyI C'CwwG_G
Cuts at: 0 189 207 1247 1428
Size: 189 18 1040 181
Fragments arranged by size:
1040 189 181 18
TatI w'GTAC_w
Cuts at: 0 119 1428
Size: 119 1309
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 283 315 1428
Size: 283 32 1113
Fragments arranged by size:
1113 283 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1383 1428
Size: 1383 45
Enzymes that do cut:
AcuI ApoI BanI BanII BfrBI Bme1580I BmrI BplI
BpmI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrDI BstYI Bsu36I BtgI DraI EaeI EarI
EciI EcoICRI Eco57MI EcoNI FspI Hin4I HincII HindIII
HpaI MmeI MscI MslI MspA1I NcoI NdeI NsiI
NspI PasI PflMI PfoI PshAI PvuII SacI ScaI
SphI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
BmgBI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsgI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgZI BtsI ClaI CspCI DraIII DrdI EagI EcoO109I
EcoRI EcoRV FalI FseI FspAI HaeII KasI KpnI
MfeI MluI NaeI NarI NgoMIV NheI NotI NruI
PacI PciI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XhoI XmaI XmnI ZraI