Restriction map for
pFN21AE2230
(Linear) (Six-base) MAPSORT of: KIEE2230.seq Check: 5536 from: 1 to: 1407
KIEE2230.orf
With 182 enzymes: SgfI *
July 3, 2012 17:16 ..
AccI GT'mk_AC
Cuts at: 0 449 1407
Size: 449 958
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 537 1105 1165 1171 1407
Size: 208 329 568 60 6 236
Fragments arranged by size:
568 329 236 208 60 6
AflII C'TTAA_G
Cuts at: 0 49 1407
Size: 49 1358
AflIII A'CryG_T
Cuts at: 0 195 1337 1407
Size: 195 1142 70
Fragments arranged by size:
1142 195 70
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 233 265 1407
Size: 233 32 1142
Fragments arranged by size:
1142 233 32
AlwNI CAG_nnn'CTG
Cuts at: 0 733 1083 1407
Size: 733 350 324
Fragments arranged by size:
733 350 324
ApaI G_GGCC'C
Cuts at: 0 1161 1407
Size: 1161 246
ApaLI G'TGCA_C
Cuts at: 0 1116 1407
Size: 1116 291
ApoI r'AATT_y
Cuts at: 0 490 657 852 956 1168 1384 1407
Size: 490 167 195 104 212 216 23
Fragments arranged by size:
490 216 212 195 167 104 23
BanII G_rGCy'C
Cuts at: 0 354 384 1161 1238 1407
Size: 354 30 777 77 169
Fragments arranged by size:
777 354 169 77 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 1340 1407
Size: 1340 67
BclI T'GATC_A
Cuts at: 0 771 1407
Size: 771 636
BfrBI ATG'CAT
Cuts at: 0 768 1407
Size: 768 639
Bme1580I G_kGCm'C
Cuts at: 0 1120 1161 1407
Size: 1120 41 246
Fragments arranged by size:
1120 246 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 348 1407
Size: 348 1059
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1252 1407
Size: 1252 155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 722 755 1407
Size: 452 270 33 652
Fragments arranged by size:
652 452 270 33
BsaI GGTCTCn'nnnn_
Cuts at: 0 21 987 1407
Size: 21 966 420
Fragments arranged by size:
966 420 21
BsaAI yAC'GTr
Cuts at: 0 1115 1407
Size: 1115 292
BsaWI w'CCGG_w
Cuts at: 0 1293 1407
Size: 1293 114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 371 401 1407
Size: 371 30 1006
Fragments arranged by size:
1006 371 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 392 1226 1407
Size: 392 834 181
Fragments arranged by size:
834 392 181
BseYI C'CCAG_C
Cuts at: 0 1231 1407
Size: 1231 176
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 1407
Size: 746 661
BsiHKAI G_wGCw'C
Cuts at: 0 1120 1407
Size: 1120 287
BsmI GAATG_Cn'
Cuts at: 0 837 1407
Size: 837 570
Bsp1286I G_dGCh'C
Cuts at: 0 354 384 1120 1161 1238 1407
Size: 354 30 736 41 77 169
Fragments arranged by size:
736 354 169 77 41 30
BsrDI GCAATG_nn'
Cuts at: 0 369 752 1407
Size: 369 383 655
Fragments arranged by size:
655 383 369
BstZ17I GTA'TAC
Cuts at: 0 450 1407
Size: 450 957
Bsu36I CC'TnA_GG
Cuts at: 0 32 1407
Size: 32 1375
BtsI GCAGTG_nn'
Cuts at: 0 1064 1407
Size: 1064 343
EaeI y'GGCC_r
Cuts at: 0 1130 1407
Size: 1130 277
EarI CTCTTCn'nnn_
Cuts at: 0 1066 1323 1407
Size: 1066 257 84
Fragments arranged by size:
1066 257 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 452 537 722 755 1105 1165 1171
Size: 208 244 85 185 33 350 60 6
Cuts at: 1171 1407
Size: 236
Fragments arranged by size:
350 244 236 208 185 85 60 33
6
EcoO109I rG'GnC_Cy
Cuts at: 0 1158 1407
Size: 1158 249
EcoRI G'AATT_C
Cuts at: 0 852 956 1407
Size: 852 104 451
Fragments arranged by size:
852 451 104
EcoRV GAT'ATC
Cuts at: 0 20 1407
Size: 20 1387
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 75 529 1407
Size: 75 454 878
Fragments arranged by size:
878 454 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 371 403 1294 1326 1407
Size: 371 32 891 32 81
Fragments arranged by size:
891 371 81 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 327 1407
Size: 327 1080
MslI CAynn'nnrTG
Cuts at: 0 389 589 1303 1407
Size: 389 200 714 104
Fragments arranged by size:
714 389 200 104
MspA1I CmG'CkG
Cuts at: 0 1072 1080 1260 1407
Size: 1072 8 180 147
Fragments arranged by size:
1072 180 147 8
NsiI A_TGCA'T
Cuts at: 0 770 1407
Size: 770 637
NspI r_CATG'y
Cuts at: 0 199 1341 1407
Size: 199 1142 66
Fragments arranged by size:
1142 199 66
PciI A'CATG_T
Cuts at: 0 195 1337 1407
Size: 195 1142 70
Fragments arranged by size:
1142 195 70
PmlI CAC'GTG
Cuts at: 0 1115 1407
Size: 1115 292
PspOMI G'GGCC_C
Cuts at: 0 1157 1407
Size: 1157 250
PstI C_TGCA'G
Cuts at: 0 1071 1265 1407
Size: 1071 194 142
Fragments arranged by size:
1071 194 142
PvuII CAG'CTG
Cuts at: 0 1080 1260 1407
Size: 1080 180 147
Fragments arranged by size:
1080 180 147
SfcI C'TryA_G
Cuts at: 0 975 1067 1261 1364 1407
Size: 975 92 194 103 43
Fragments arranged by size:
975 194 103 92 43
SmlI C'TyrA_G
Cuts at: 0 49 1407
Size: 49 1358
SspI AAT'ATT
Cuts at: 0 318 1407
Size: 318 1089
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 968 1407
Size: 968 439
TatI w'GTAC_w
Cuts at: 0 189 630 684 1009 1407
Size: 189 441 54 325 398
Fragments arranged by size:
441 398 325 189 54
XbaI T'CTAG_A
Cuts at: 0 714 1407
Size: 714 693
Enzymes that do cut:
AccI AcuI AflII AflIII AloI AlwNI ApaI ApaLI
ApoI BanII BbsI BclI BfrBI Bme1580I BmrI BplI
BpmI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BsrDI BstZ17I Bsu36I BtsI EaeI
EarI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I MmeI
MslI MspA1I NsiI NspI PciI PmlI PspOMI PstI
PvuII SfcI SmlI SspI TaqII TatI XbaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BciVI BglI BglII BlpI
BmgBI BmtI Bpu10I BpuEI BsaBI BsaHI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI StyI SwaI TstI Tth111I
XcmI XhoI XmaI XmnI ZraI