Restriction map for
pFN21AE2227
(Linear) (Six-base) MAPSORT of: KIEE2227.seq Check: 4326 from: 1 to: 1167
KIEE2227.orf
With 182 enzymes: SgfI *
July 15, 2010 18:56 ..
Acc65I G'GTAC_C
Cuts at: 0 739 1058 1167
Size: 739 319 109
Fragments arranged by size:
739 319 109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 1167
Size: 755 412
AlwNI CAG_nnn'CTG
Cuts at: 0 472 508 1027 1167
Size: 472 36 519 140
Fragments arranged by size:
519 472 140 36
ApoI r'AATT_y
Cuts at: 0 409 663 1068 1167
Size: 409 254 405 99
Fragments arranged by size:
409 405 254 99
AvaI C'yCGr_G
Cuts at: 0 450 1167
Size: 450 717
BanI G'GyrC_C
Cuts at: 0 46 634 739 1058 1167
Size: 46 588 105 319 109
Fragments arranged by size:
588 319 109 105 46
BbeI G_GCGC'C
Cuts at: 0 50 1167
Size: 50 1117
BfrBI ATG'CAT
Cuts at: 0 1160 1167
Size: 1160 7
BglI GCCn_nnn'nGGC
Cuts at: 0 141 181 1167
Size: 141 40 986
Fragments arranged by size:
986 141 40
BmrI ACTGGGnnnn_n'
Cuts at: 0 66 434 640 1167
Size: 66 368 206 527
Fragments arranged by size:
527 368 206 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 262 365 530 1167
Size: 102 160 103 165 637
Fragments arranged by size:
637 165 160 103 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 991 1167
Size: 991 176
BsaHI Gr'CG_yC
Cuts at: 0 47 1167
Size: 47 1120
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 51 81 488 518 1167
Size: 51 30 407 30 649
Fragments arranged by size:
649 407 51 30 30
BseYI C'CCAG_C
Cuts at: 0 98 465 1167
Size: 98 367 702
Fragments arranged by size:
702 367 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 322 1167
Size: 181 141 845
Fragments arranged by size:
845 181 141
BsiHKAI G_wGCw'C
Cuts at: 0 84 152 598 1167
Size: 84 68 446 569
Fragments arranged by size:
569 446 84 68
Bsp1286I G_dGCh'C
Cuts at: 0 84 152 598 1167
Size: 84 68 446 569
Fragments arranged by size:
569 446 84 68
BsrDI GCAATG_nn'
Cuts at: 0 690 1167
Size: 690 477
BstYI r'GATC_y
Cuts at: 0 340 1167
Size: 340 827
BtsI GCAGTG_nn'
Cuts at: 0 596 623 1167
Size: 596 27 544
Fragments arranged by size:
596 544 27
DrdI GACnn_nn'nnGTC
Cuts at: 0 121 1167
Size: 121 1046
EaeI y'GGCC_r
Cuts at: 0 328 378 1167
Size: 328 50 789
Fragments arranged by size:
789 328 50
EarI CTCTTCn'nnn_
Cuts at: 0 157 218 1167
Size: 157 61 949
Fragments arranged by size:
949 157 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 262 365 530 755 1167
Size: 102 160 103 165 225 412
Fragments arranged by size:
412 225 165 160 103 102
EcoO109I rG'GnC_Cy
Cuts at: 0 28 1167
Size: 28 1139
HaeII r_GCGC'y
Cuts at: 0 21 50 187 196 1167
Size: 21 29 137 9 971
Fragments arranged by size:
971 137 29 21 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 51 83 325 357 1167
Size: 51 32 242 32 810
Fragments arranged by size:
810 242 51 32 32
HincII GTy'rAC
Cuts at: 0 873 1167
Size: 873 294
HpaI GTT'AAC
Cuts at: 0 873 1167
Size: 873 294
KasI G'GCGC_C
Cuts at: 0 46 1167
Size: 46 1121
KpnI G_GTAC'C
Cuts at: 0 743 1062 1167
Size: 743 319 105
Fragments arranged by size:
743 319 105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 961 1141 1167
Size: 339 622 180 26
Fragments arranged by size:
622 339 180 26
MspA1I CmG'CkG
Cuts at: 0 36 1167
Size: 36 1131
NarI GG'CG_CC
Cuts at: 0 47 1167
Size: 47 1120
NsiI A_TGCA'T
Cuts at: 0 1162 1167
Size: 1162 5
NspI r_CATG'y
Cuts at: 0 645 1167
Size: 645 522
PfoI T'CCnGG_A
Cuts at: 0 921 1167
Size: 921 246
PpuMI rG'GwC_Cy
Cuts at: 0 28 1167
Size: 28 1139
SapI GCTCTTCn'nnn_
Cuts at: 0 157 1167
Size: 157 1010
SfoI GGC'GCC
Cuts at: 0 48 1167
Size: 48 1119
SmlI C'TyrA_G
Cuts at: 0 1006 1167
Size: 1006 161
SspI AAT'ATT
Cuts at: 0 782 1167
Size: 782 385
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 603 622 1167
Size: 603 19 545
Fragments arranged by size:
603 545 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 269 301 1167
Size: 269 32 866
Fragments arranged by size:
866 269 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 852 1167
Size: 852 315
XmnI GAAnn'nnTTC
Cuts at: 0 575 1167
Size: 575 592
Enzymes that do cut:
Acc65I AcuI AlwNI ApoI AvaI BanI BbeI BfrBI
BglI BmrI BpmI BpuEI BsaHI BsaXI BseYI BsgI
BsiHKAI Bsp1286I BsrDI BstYI BtsI DrdI EaeI EarI
Eco57MI EcoO109I HaeII Hin4I HincII HpaI KasI KpnI
MmeI MspA1I NarI NsiI NspI PfoI PpuMI SapI
SfoI SmlI SspI TaqII TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbsI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmgBI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaWI BseRI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DraIII EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HindIII MfeI MluI MscI MslI NaeI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI StyI SwaI TatI
Tth111I XbaI XhoI XmaI ZraI