Restriction map for
pFN21AE2226
(Linear) (Six-base) MAPSORT of: KIEE2226.seq Check: 8936 from: 1 to: 1059
KIEE2226.orf
With 182 enzymes: SgfI *
July 3, 2012 17:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1010 1059
Size: 1010 49
AflIII A'CryG_T
Cuts at: 0 599 1059
Size: 599 460
AhdI GACnn_n'nnGTC
Cuts at: 0 35 170 1059
Size: 35 135 889
Fragments arranged by size:
889 135 35
AleI CACnn'nnGTG
Cuts at: 0 350 1059
Size: 350 709
AlwNI CAG_nnn'CTG
Cuts at: 0 318 1059
Size: 318 741
ApoI r'AATT_y
Cuts at: 0 471 890 1059
Size: 471 419 169
Fragments arranged by size:
471 419 169
BamHI G'GATC_C
Cuts at: 0 455 462 693 1059
Size: 455 7 231 366
Fragments arranged by size:
455 366 231 7
BanI G'GyrC_C
Cuts at: 0 129 839 1059
Size: 129 710 220
Fragments arranged by size:
710 220 129
BanII G_rGCy'C
Cuts at: 0 373 415 781 998 1059
Size: 373 42 366 217 61
Fragments arranged by size:
373 366 217 61 42
BbeI G_GCGC'C
Cuts at: 0 843 1059
Size: 843 216
BbsI GAAGACnn'nnnn_
Cuts at: 0 1016 1059
Size: 1016 43
BbvCI CC'TCA_GC
Cuts at: 0 978 1059
Size: 978 81
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 27 1059
Size: 27 1032
BglI GCCn_nnn'nGGC
Cuts at: 0 309 406 1059
Size: 309 97 653
Fragments arranged by size:
653 309 97
BlpI GC'TnA_GC
Cuts at: 0 103 997 1007 1059
Size: 103 894 10 52
Fragments arranged by size:
894 103 52 10
BmgBI CAC'GTC
Cuts at: 0 600 1059
Size: 600 459
BmrI ACTGGGnnnn_n'
Cuts at: 0 693 1059
Size: 693 366
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 205 539 1059
Size: 166 39 334 520
Fragments arranged by size:
520 334 166 39
Bpu10I CC'TnA_GC
Cuts at: 0 780 978 1059
Size: 780 198 81
Fragments arranged by size:
780 198 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 1059
Size: 880 179
BsaBI GATnn'nnATC
Cuts at: 0 715 1059
Size: 715 344
BsaHI Gr'CG_yC
Cuts at: 0 840 1059
Size: 840 219
BsaWI w'CCGG_w
Cuts at: 0 266 622 1059
Size: 266 356 437
Fragments arranged by size:
437 356 266
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 139 169 589 619 1059
Size: 139 30 420 30 440
Fragments arranged by size:
440 420 139 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 811 1059
Size: 112 699 248
Fragments arranged by size:
699 248 112
BseYI C'CCAG_C
Cuts at: 0 235 428 644 765 1059
Size: 235 193 216 121 294
Fragments arranged by size:
294 235 216 193 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 1059
Size: 820 239
BsiEI CG_ry'CG
Cuts at: 0 17 1059
Size: 17 1042
BsmBI CGTCTCn'nnnn_
Cuts at: 0 879 1059
Size: 879 180
Bsp1286I G_dGCh'C
Cuts at: 0 373 415 781 998 1059
Size: 373 42 366 217 61
Fragments arranged by size:
373 366 217 61 42
BspEI T'CCGG_A
Cuts at: 0 266 1059
Size: 266 793
BspHI T'CATG_A
Cuts at: 0 719 1059
Size: 719 340
BsrBI CCG'CTC
Cuts at: 0 84 200 549 1059
Size: 84 116 349 510
Fragments arranged by size:
510 349 116 84
BsrDI GCAATG_nn'
Cuts at: 0 4 1059
Size: 4 1055
BsrFI r'CCGG_y
Cuts at: 0 664 1059
Size: 664 395
BssSI C'ACGA_G
Cuts at: 0 658 1059
Size: 658 401
BstYI r'GATC_y
Cuts at: 0 455 462 693 710 1059
Size: 455 7 231 17 349
Fragments arranged by size:
455 349 231 17 7
DraIII CAC_nnn'GTG
Cuts at: 0 329 1059
Size: 329 730
EaeI y'GGCC_r
Cuts at: 0 272 662 1059
Size: 272 390 397
Fragments arranged by size:
397 390 272
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 205 539 1010 1059
Size: 166 39 334 471 49
Fragments arranged by size:
471 334 166 49 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 220 981 1059
Size: 220 761 78
Fragments arranged by size:
761 220 78
EcoRI G'AATT_C
Cuts at: 0 890 1059
Size: 890 169
HaeII r_GCGC'y
Cuts at: 0 843 1059
Size: 843 216
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 21 53 156 188 447 479 589 621
Size: 21 32 103 32 259 32 110 32
Cuts at: 621 685 717 1059
Size: 64 32 342
Fragments arranged by size:
342 259 110 103 64 32 32 32
32 32 21
KasI G'GCGC_C
Cuts at: 0 839 1059
Size: 839 220
MslI CAynn'nnrTG
Cuts at: 0 350 364 1059
Size: 350 14 695
Fragments arranged by size:
695 350 14
MspA1I CmG'CkG
Cuts at: 0 375 542 846 1059
Size: 375 167 304 213
Fragments arranged by size:
375 304 213 167
NaeI GCC'GGC
Cuts at: 0 666 1059
Size: 666 393
NarI GG'CG_CC
Cuts at: 0 840 1059
Size: 840 219
NgoMIV G'CCGG_C
Cuts at: 0 664 1059
Size: 664 395
PasI CC'CwG_GG
Cuts at: 0 1015 1059
Size: 1015 44
PfoI T'CCnGG_A
Cuts at: 0 458 852 1059
Size: 458 394 207
Fragments arranged by size:
458 394 207
PshAI GACnn'nnGTC
Cuts at: 0 861 1059
Size: 861 198
PstI C_TGCA'G
Cuts at: 0 188 245 888 1059
Size: 188 57 643 171
Fragments arranged by size:
643 188 171 57
PvuII CAG'CTG
Cuts at: 0 542 846 1059
Size: 542 304 213
Fragments arranged by size:
542 304 213
SexAI A'CCwGG_T
Cuts at: 0 125 338 1059
Size: 125 213 721
Fragments arranged by size:
721 213 125
SfcI C'TryA_G
Cuts at: 0 184 241 884 1059
Size: 184 57 643 175
Fragments arranged by size:
643 184 175 57
SfoI GGC'GCC
Cuts at: 0 841 1059
Size: 841 218
SmlI C'TyrA_G
Cuts at: 0 859 1059
Size: 859 200
StuI AGG'CCT
Cuts at: 0 805 1059
Size: 805 254
StyI C'CwwG_G
Cuts at: 0 388 1059
Size: 388 671
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1028 1059
Size: 1028 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 313 345 678 710 1059
Size: 313 32 333 32 349
Fragments arranged by size:
349 333 313 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 850 1059
Size: 850 209
XmnI GAAnn'nnTTC
Cuts at: 0 925 1059
Size: 925 134
Enzymes that do cut:
AcuI AflIII AhdI AleI AlwNI ApoI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BglI BlpI BmgBI
BmrI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspEI BspHI
BsrBI BsrDI BsrFI BssSI BstYI DraIII EaeI Eco57MI
EcoNI EcoRI HaeII Hin4I KasI MslI MspA1I NaeI
NarI NgoMIV PasI PfoI PshAI PstI PvuII SexAI
SfcI SfoI SmlI StuI StyI TaqII TstI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BciVI BclI BfrBI BglII Bme1580I BmtI
BplI BsaI BsaAI BsiHKAI BsiWI BsmI BspMI BsrGI
BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EagI EarI
EciI EcoICRI EcoO109I EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI
XhoI XmaI ZraI