Restriction map for
pFN21AE2221
(Linear) (Six-base) MAPSORT of: KIEE2221.seq Check: 1413 from: 1 to: 1005
KIEE2221.orf
With 182 enzymes: SgfI *
July 15, 2010 18:56 ..
AccI GT'mk_AC
Cuts at: 0 670 1005
Size: 670 335
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 309 1005
Size: 119 190 696
Fragments arranged by size:
696 190 119
AfeI AGC'GCT
Cuts at: 0 386 1005
Size: 386 619
AflIII A'CryG_T
Cuts at: 0 701 1005
Size: 701 304
AhdI GACnn_n'nnGTC
Cuts at: 0 554 1005
Size: 554 451
AvaI C'yCGr_G
Cuts at: 0 641 1005
Size: 641 364
BamHI G'GATC_C
Cuts at: 0 713 849 957 1005
Size: 713 136 108 48
Fragments arranged by size:
713 136 108 48
BanII G_rGCy'C
Cuts at: 0 407 804 1005
Size: 407 397 201
Fragments arranged by size:
407 397 201
BbsI GAAGACnn'nnnn_
Cuts at: 0 125 1005
Size: 125 880
BbvCI CC'TCA_GC
Cuts at: 0 101 1005
Size: 101 904
BfrBI ATG'CAT
Cuts at: 0 979 1005
Size: 979 26
Bme1580I G_kGCm'C
Cuts at: 0 68 1005
Size: 68 937
BmrI ACTGGGnnnn_n'
Cuts at: 0 649 1005
Size: 649 356
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 34 1005
Size: 34 971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 1005
Size: 391 614
Bpu10I CC'TnA_GC
Cuts at: 0 101 458 1005
Size: 101 357 547
Fragments arranged by size:
547 357 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 1005
Size: 300 705
BsaI GGTCTCn'nnnn_
Cuts at: 0 333 652 1005
Size: 333 319 353
Fragments arranged by size:
353 333 319
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 437 467 551 581 1005
Size: 437 30 84 30 424
Fragments arranged by size:
437 424 84 30 30
BseYI C'CCAG_C
Cuts at: 0 791 1005
Size: 791 214
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 1005
Size: 70 935
BsiHKAI G_wGCw'C
Cuts at: 0 804 1005
Size: 804 201
BsmI GAATG_Cn'
Cuts at: 0 981 1005
Size: 981 24
Bsp1286I G_dGCh'C
Cuts at: 0 68 407 804 1005
Size: 68 339 397 201
Fragments arranged by size:
397 339 201 68
BspHI T'CATG_A
Cuts at: 0 435 1005
Size: 435 570
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 92 1005
Size: 92 913
BsrGI T'GTAC_A
Cuts at: 0 884 1005
Size: 884 121
BstXI CCAn_nnnn'nTGG
Cuts at: 0 378 1005
Size: 378 627
BstYI r'GATC_y
Cuts at: 0 713 849 957 1005
Size: 713 136 108 48
Fragments arranged by size:
713 136 108 48
BstZ17I GTA'TAC
Cuts at: 0 671 1005
Size: 671 334
BtgI C'CryG_G
Cuts at: 0 901 962 1005
Size: 901 61 43
Fragments arranged by size:
901 61 43
ClaI AT'CG_AT
Cuts at: 0 489 1005
Size: 489 516
EaeI y'GGCC_r
Cuts at: 0 59 1005
Size: 59 946
EcoICRI GAG'CTC
Cuts at: 0 802 1005
Size: 802 203
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 309 391 1005
Size: 119 190 82 614
Fragments arranged by size:
614 190 119 82
HaeII r_GCGC'y
Cuts at: 0 388 792 1005
Size: 388 404 213
Fragments arranged by size:
404 388 213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 152 184 273 305 330 362 504 536
Size: 152 32 89 32 25 32 142 32
Cuts at: 536 540 572 1005
Size: 4 32 433
Fragments arranged by size:
433 152 142 89 32 32 32 32
32 25 4
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 259 1005
Size: 177 82 746
Fragments arranged by size:
746 177 82
MslI CAynn'nnrTG
Cuts at: 0 440 1005
Size: 440 565
MspA1I CmG'CkG
Cuts at: 0 903 1005
Size: 903 102
NcoI C'CATG_G
Cuts at: 0 962 1005
Size: 962 43
NsiI A_TGCA'T
Cuts at: 0 981 1005
Size: 981 24
NspI r_CATG'y
Cuts at: 0 705 1005
Size: 705 300
PciI A'CATG_T
Cuts at: 0 701 1005
Size: 701 304
PflMI CCAn_nnn'nTGG
Cuts at: 0 661 1005
Size: 661 344
SacI G_AGCT'C
Cuts at: 0 804 1005
Size: 804 201
SacII CC_GC'GG
Cuts at: 0 904 1005
Size: 904 101
SfcI C'TryA_G
Cuts at: 0 471 1005
Size: 471 534
SmlI C'TyrA_G
Cuts at: 0 279 641 1005
Size: 279 362 364
Fragments arranged by size:
364 362 279
StyI C'CwwG_G
Cuts at: 0 962 1005
Size: 962 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 178 618 783 1005
Size: 178 440 165 222
Fragments arranged by size:
440 222 178 165
TatI w'GTAC_w
Cuts at: 0 758 884 1005
Size: 758 126 121
Fragments arranged by size:
758 126 121
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 551 583 1005
Size: 551 32 422
Fragments arranged by size:
551 422 32
XhoI C'TCGA_G
Cuts at: 0 641 1005
Size: 641 364
XmnI GAAnn'nnTTC
Cuts at: 0 778 1005
Size: 778 227
Enzymes that do cut:
AccI AcuI AfeI AflIII AhdI AvaI BamHI BanII
BbsI BbvCI BfrBI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrGI BstXI BstYI BstZ17I BtgI ClaI
EaeI EcoICRI Eco57MI HaeII Hin4I MmeI MslI MspA1I
NcoI NsiI NspI PciI PflMI SacI SacII SfcI
SmlI StyI TaqII TatI TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BanI BbeI BcgI BciVI BclI BglI
BglII BlpI BmgBI BmtI BsaAI BsaBI BsaHI BsaWI
BseRI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII Bsu36I BtgZI BtsI
CspCI DraI DraIII DrdI EagI EarI EciI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XmaI ZraI