(Linear) (Six-base) MAPSORT of: KIEE2220.seq  Check: 6378  from: 1  to: 522



KIEE2220.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:55  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      95     522 
   Size:         95     427
 
BanI G'GyrC_C

Cuts at:      0     458     522 
   Size:        458      64
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     293     326     522 
   Size:        293      33     196

  Fragments arranged by size: 

                 293     196      33
 
BbvCI CC'TCA_GC

Cuts at:      0     214     352     436     522 
   Size:        214     138      84      86

  Fragments arranged by size: 

                 214     138      86      84
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     477     522 
   Size:        477      45
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     522 
   Size:        400     122
 
Bpu10I CC'TnA_GC

Cuts at:      0     214     352     436     485     522 
   Size:        214     138      84      49      37

  Fragments arranged by size: 

                 214     138      84      49      37
 
BsaAI yAC'GTr

Cuts at:      0     375     522 
   Size:        375     147
 
BseYI C'CCAG_C

Cuts at:      0     328     389     522 
   Size:        328      61     133

  Fragments arranged by size: 

                 328     133      61
 
BspHI T'CATG_A

Cuts at:      0     302     522 
   Size:        302     220
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      83     440     522 
   Size:         83     357      82

  Fragments arranged by size: 

                 357      83      82
 
BsrBI CCG'CTC

Cuts at:      0     119     230     522 
   Size:        119     111     292

  Fragments arranged by size: 

                 292     119     111
 
BsrDI GCAATG_nn'

Cuts at:      0     208     522 
   Size:        208     314
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     432     522 
   Size:        432      90
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     111     522 
   Size:        111     411
 
BstYI r'GATC_y

Cuts at:      0     359     522 
   Size:        359     163
 
BtgI C'CryG_G

Cuts at:      0     476     522 
   Size:        476      46
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     522 
   Size:        400     122
 
EcoO109I rG'GnC_Cy

Cuts at:      0      29     393     440     522 
   Size:         29     364      47      82

  Fragments arranged by size: 

                 364      82      47      29
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      30     522 
   Size:         30     492
 
HincII GTy'rAC

Cuts at:      0     160     522 
   Size:        160     362
 
MslI CAynn'nnrTG

Cuts at:      0     109     522 
   Size:        109     413
 
MspA1I CmG'CkG

Cuts at:      0      95     522 
   Size:         95     427
 
NcoI C'CATG_G

Cuts at:      0     476     522 
   Size:        476      46
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     378     522 
   Size:        378     144
 
PfoI T'CCnGG_A

Cuts at:      0     335     522 
   Size:        335     187
 
PmlI CAC'GTG

Cuts at:      0     375     522 
   Size:        375     147
 
PpuMI rG'GwC_Cy

Cuts at:      0     393     522 
   Size:        393     129
 
PstI C_TGCA'G

Cuts at:      0      80     522 
   Size:         80     442
 
SfcI C'TryA_G

Cuts at:      0      76     522 
   Size:         76     446
 
StuI AGG'CCT

Cuts at:      0     299     522 
   Size:        299     223
 
StyI C'CwwG_G

Cuts at:      0     153     476     522 
   Size:        153     323      46

  Fragments arranged by size: 

                 323     153      46
 
TatI w'GTAC_w

Cuts at:      0     449     522 
   Size:        449      73
 

 Enzymes that do cut:

    AlwNI     BanI     BbsI    BbvCI     BmrI     BpmI   Bpu10I    BsaAI
    BseYI    BspHI    BspMI    BsrBI    BsrDI   BstAPI    BstXI    BstYI
     BtgI  Eco57MI EcoO109I     FalI   HincII     MslI   MspA1I     NcoI
    PflMI     PfoI     PmlI    PpuMI     PstI     SfcI     StuI     StyI
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbeI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PmeI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI