Restriction map for
pFN21AE2217
(Linear) (Six-base) MAPSORT of: KIEE2217.seq Check: 622 from: 1 to: 879
KIEE2217.orf
With 182 enzymes: SgfI *
July 15, 2010 18:55 ..
AccI GT'mk_AC
Cuts at: 0 446 879
Size: 446 433
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 879
Size: 335 544
ApoI r'AATT_y
Cuts at: 0 86 722 879
Size: 86 636 157
Fragments arranged by size:
636 157 86
BamHI G'GATC_C
Cuts at: 0 688 879
Size: 688 191
BanI G'GyrC_C
Cuts at: 0 591 879
Size: 591 288
BbeI G_GCGC'C
Cuts at: 0 595 879
Size: 595 284
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 202 236 879
Size: 202 34 643
Fragments arranged by size:
643 202 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 105 879
Size: 105 774
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 164 879
Size: 164 715
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 400 879
Size: 361 39 479
Fragments arranged by size:
479 361 39
BsaHI Gr'CG_yC
Cuts at: 0 592 879
Size: 592 287
BseYI C'CCAG_C
Cuts at: 0 512 879
Size: 512 367
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 785 879
Size: 785 94
BsiEI CG_ry'CG
Cuts at: 0 821 879
Size: 821 58
BssSI C'ACGA_G
Cuts at: 0 211 879
Size: 211 668
BstYI r'GATC_y
Cuts at: 0 688 698 879
Size: 688 10 181
Fragments arranged by size:
688 181 10
Bsu36I CC'TnA_GG
Cuts at: 0 308 879
Size: 308 571
BtgI C'CryG_G
Cuts at: 0 419 587 879
Size: 419 168 292
Fragments arranged by size:
419 292 168
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 837 872 879
Size: 837 35 7
Fragments arranged by size:
837 35 7
DraI TTT'AAA
Cuts at: 0 738 879
Size: 738 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 361 400 879
Size: 335 26 39 479
Fragments arranged by size:
479 335 39 26
EcoO109I rG'GnC_Cy
Cuts at: 0 860 879
Size: 860 19
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 386 418 645 879
Size: 386 32 227 234
Fragments arranged by size:
386 234 227 32
HaeII r_GCGC'y
Cuts at: 0 595 879
Size: 595 284
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 222 254 879
Size: 222 32 625
Fragments arranged by size:
625 222 32
HincII GTy'rAC
Cuts at: 0 447 879
Size: 447 432
KasI G'GCGC_C
Cuts at: 0 591 879
Size: 591 288
MslI CAynn'nnrTG
Cuts at: 0 174 879
Size: 174 705
MspA1I CmG'CkG
Cuts at: 0 339 516 589 879
Size: 339 177 73 290
Fragments arranged by size:
339 290 177 73
NarI GG'CG_CC
Cuts at: 0 592 879
Size: 592 287
NcoI C'CATG_G
Cuts at: 0 419 879
Size: 419 460
NspI r_CATG'y
Cuts at: 0 333 879
Size: 333 546
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 567 599 879
Size: 567 32 280
Fragments arranged by size:
567 280 32
PpuMI rG'GwC_Cy
Cuts at: 0 860 879
Size: 860 19
PshAI GACnn'nnGTC
Cuts at: 0 865 879
Size: 865 14
PvuI CG_AT'CG
Cuts at: 0 821 879
Size: 821 58
PvuII CAG'CTG
Cuts at: 0 339 516 879
Size: 339 177 363
Fragments arranged by size:
363 339 177
SacII CC_GC'GG
Cuts at: 0 590 879
Size: 590 289
SalI G'TCGA_C
Cuts at: 0 445 879
Size: 445 434
SanDI GG'GwC_CC
Cuts at: 0 860 879
Size: 860 19
ScaI AGT'ACT
Cuts at: 0 253 879
Size: 253 626
SfoI GGC'GCC
Cuts at: 0 593 879
Size: 593 286
SspI AAT'ATT
Cuts at: 0 795 879
Size: 795 84
StyI C'CwwG_G
Cuts at: 0 224 419 879
Size: 224 195 460
Fragments arranged by size:
460 224 195
TatI w'GTAC_w
Cuts at: 0 127 251 879
Size: 127 124 628
Fragments arranged by size:
628 127 124
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 199 231 879
Size: 199 32 648
Fragments arranged by size:
648 199 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 426 879
Size: 426 453
XmnI GAAnn'nnTTC
Cuts at: 0 398 794 879
Size: 398 396 85
Fragments arranged by size:
398 396 85
Enzymes that do cut:
AccI AcuI ApoI BamHI BanI BbeI BcgI BciVI
BplI BpmI BsaHI BseYI BsgI BsiEI BssSI BstYI
Bsu36I BtgI CspCI DraI Eco57MI EcoO109I FalI HaeII
Hin4I HincII KasI MslI MspA1I NarI NcoI NspI
PpiI PpuMI PshAI PvuI PvuII SacII SalI SanDI
ScaI SfoI SspI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BanII BbsI BbvCI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI
BseRI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstZ17I BtgZI BtsI ClaI DraIII DrdI
EaeI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
MmeI MscI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PsiI PspOMI PspXI PsrI PstI RsrII SacI SapI
SbfI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI SwaI TaqII Tth111I
XbaI XhoI XmaI ZraI