Restriction map for
pFN21AE2215
(Linear) (Six-base) MAPSORT of: KIEE2215.seq Check: 9681 from: 1 to: 1356
KIEE2215.orf
With 182 enzymes: SgfI *
July 15, 2010 18:55 ..
AccI GT'mk_AC
Cuts at: 0 100 1124 1356
Size: 100 1024 232
Fragments arranged by size:
1024 232 100
Acc65I G'GTAC_C
Cuts at: 0 722 1356
Size: 722 634
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 510 1356
Size: 510 846
AflIII A'CryG_T
Cuts at: 0 809 1262 1356
Size: 809 453 94
Fragments arranged by size:
809 453 94
AgeI A'CCGG_T
Cuts at: 0 767 938 1356
Size: 767 171 418
Fragments arranged by size:
767 418 171
ApaI G_GGCC'C
Cuts at: 0 599 834 1356
Size: 599 235 522
Fragments arranged by size:
599 522 235
ApoI r'AATT_y
Cuts at: 0 247 1356
Size: 247 1109
AvaI C'yCGr_G
Cuts at: 0 827 1356
Size: 827 529
BanI G'GyrC_C
Cuts at: 0 29 379 722 1356
Size: 29 350 343 634
Fragments arranged by size:
634 350 343 29
BanII G_rGCy'C
Cuts at: 0 534 599 834 1295 1356
Size: 534 65 235 461 61
Fragments arranged by size:
534 461 235 65 61
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 330 364 1356
Size: 330 34 992
Fragments arranged by size:
992 330 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 498 1356
Size: 498 858
BclI T'GATC_A
Cuts at: 0 1166 1356
Size: 1166 190
BglI GCCn_nnn'nGGC
Cuts at: 0 122 463 1356
Size: 122 341 893
Fragments arranged by size:
893 341 122
Bme1580I G_kGCm'C
Cuts at: 0 382 599 834 1356
Size: 382 217 235 522
Fragments arranged by size:
522 382 235 217
BmgBI CAC'GTC
Cuts at: 0 285 1356
Size: 285 1071
BmrI ACTGGGnnnn_n'
Cuts at: 0 1090 1356
Size: 1090 266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 1003 1019 1072 1320 1356
Size: 274 729 16 53 248 36
Fragments arranged by size:
729 274 248 53 36 16
BsaI GGTCTCn'nnnn_
Cuts at: 0 1090 1274 1356
Size: 1090 184 82
Fragments arranged by size:
1090 184 82
BsaAI yAC'GTr
Cuts at: 0 1263 1356
Size: 1263 93
BsaWI w'CCGG_w
Cuts at: 0 602 620 767 938 1356
Size: 602 18 147 171 418
Fragments arranged by size:
602 418 171 147 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 652 682 697 727 1070 1100 1356
Size: 652 30 15 30 343 30 256
Fragments arranged by size:
652 343 256 30 30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 673 697 1356
Size: 673 24 659
Fragments arranged by size:
673 659 24
BseYI C'CCAG_C
Cuts at: 0 507 611 858 1356
Size: 507 104 247 498
Fragments arranged by size:
507 498 247 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 1356
Size: 688 668
BsiEI CG_ry'CG
Cuts at: 0 121 426 479 1356
Size: 121 305 53 877
Fragments arranged by size:
877 305 121 53
BsiHKAI G_wGCw'C
Cuts at: 0 290 899 1295 1356
Size: 290 609 396 61
Fragments arranged by size:
609 396 290 61
Bsp1286I G_dGCh'C
Cuts at: 0 290 382 534 599 834 899 1295 1356
Size: 290 92 152 65 235 65 396 61
Fragments arranged by size:
396 290 235 152 92 65 65 61
BspEI T'CCGG_A
Cuts at: 0 620 1356
Size: 620 736
BsrFI r'CCGG_y
Cuts at: 0 116 767 938 1158 1356
Size: 116 651 171 220 198
Fragments arranged by size:
651 220 198 171 116
BstEII G'GTnAC_C
Cuts at: 0 846 1231 1356
Size: 846 385 125
Fragments arranged by size:
846 385 125
BstXI CCAn_nnnn'nTGG
Cuts at: 0 981 1080 1356
Size: 981 99 276
Fragments arranged by size:
981 276 99
BstYI r'GATC_y
Cuts at: 0 905 1356
Size: 905 451
BtgI C'CryG_G
Cuts at: 0 40 93 1133 1356
Size: 40 53 1040 223
Fragments arranged by size:
1040 223 53 40
BtsI GCAGTG_nn'
Cuts at: 0 63 736 1356
Size: 63 673 620
Fragments arranged by size:
673 620 63
DrdI GACnn_nn'nnGTC
Cuts at: 0 577 885 1356
Size: 577 308 471
Fragments arranged by size:
577 471 308
EaeI y'GGCC_r
Cuts at: 0 118 423 464 476 1130 1356
Size: 118 305 41 12 654 226
Fragments arranged by size:
654 305 226 118 41 12
EagI C'GGCC_G
Cuts at: 0 118 423 476 1356
Size: 118 305 53 880
Fragments arranged by size:
880 305 118 53
EarI CTCTTCn'nnn_
Cuts at: 0 619 1356
Size: 619 737
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 140 1356
Size: 140 1216
EcoICRI GAG'CTC
Cuts at: 0 1293 1356
Size: 1293 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 510 1003 1019 1072 1320 1356
Size: 274 236 493 16 53 248 36
Fragments arranged by size:
493 274 248 236 53 36 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 538 603 1356
Size: 538 65 753
Fragments arranged by size:
753 538 65
EcoO109I rG'GnC_Cy
Cuts at: 0 596 608 687 831 864 1356
Size: 596 12 79 144 33 492
Fragments arranged by size:
596 492 144 79 33 12
HaeII r_GCGC'y
Cuts at: 0 1273 1356
Size: 1273 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 472 504 1356
Size: 472 32 852
Fragments arranged by size:
852 472 32
HincII GTy'rAC
Cuts at: 0 101 1125 1356
Size: 101 1024 231
Fragments arranged by size:
1024 231 101
HindIII A'AGCT_T
Cuts at: 0 361 1356
Size: 361 995
KpnI G_GTAC'C
Cuts at: 0 726 1356
Size: 726 630
MluI A'CGCG_T
Cuts at: 0 809 1356
Size: 809 547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 869 1356
Size: 869 487
MscI TGG'CCA
Cuts at: 0 1132 1356
Size: 1132 224
MslI CAynn'nnrTG
Cuts at: 0 45 1356
Size: 45 1311
MspA1I CmG'CkG
Cuts at: 0 35 520 1050 1188 1356
Size: 35 485 530 138 168
Fragments arranged by size:
530 485 168 138 35
NaeI GCC'GGC
Cuts at: 0 118 1356
Size: 118 1238
NcoI C'CATG_G
Cuts at: 0 93 1133 1356
Size: 93 1040 223
Fragments arranged by size:
1040 223 93
NgoMIV G'CCGG_C
Cuts at: 0 116 1356
Size: 116 1240
PasI CC'CwG_GG
Cuts at: 0 540 690 1356
Size: 540 150 666
Fragments arranged by size:
666 540 150
PflMI CCAn_nnn'nTGG
Cuts at: 0 546 1356
Size: 546 810
PfoI T'CCnGG_A
Cuts at: 0 290 980 1319 1356
Size: 290 690 339 37
Fragments arranged by size:
690 339 290 37
PspOMI G'GGCC_C
Cuts at: 0 595 830 1356
Size: 595 235 526
Fragments arranged by size:
595 526 235
PvuII CAG'CTG
Cuts at: 0 1050 1188 1356
Size: 1050 138 168
Fragments arranged by size:
1050 168 138
SacI G_AGCT'C
Cuts at: 0 1295 1356
Size: 1295 61
SalI G'TCGA_C
Cuts at: 0 99 1123 1356
Size: 99 1024 233
Fragments arranged by size:
1024 233 99
SmaI CCC'GGG
Cuts at: 0 829 1356
Size: 829 527
StyI C'CwwG_G
Cuts at: 0 93 1073 1133 1356
Size: 93 980 60 223
Fragments arranged by size:
980 223 93 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 697 703 729 735 1070 1102 1356
Size: 697 6 26 6 335 32 254
Fragments arranged by size:
697 335 254 32 26 6 6
Tth111I GACn'n_nGTC
Cuts at: 0 577 844 1120 1356
Size: 577 267 276 236
Fragments arranged by size:
577 276 267 236
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1218 1356
Size: 1218 138
XmaI C'CCGG_G
Cuts at: 0 827 1356
Size: 827 529
XmnI GAAnn'nnTTC
Cuts at: 0 739 1356
Size: 739 617
Enzymes that do cut:
AccI Acc65I AcuI AflIII AgeI ApaI ApoI AvaI
BanI BanII BcgI BciVI BclI BglI Bme1580I BmgBI
BmrI BpmI BsaI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BsrFI BstEII BstXI
BstYI BtgI BtsI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII
KpnI MluI MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV PasI PflMI PfoI PspOMI PvuII SacI SalI
SmaI StyI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AhdI AleI AlfI
AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BfrBI BglII BlpI BmtI
BplI Bpu10I BpuEI BsaBI BsaHI BsiWI BsmI BsmBI
BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
EcoRI EcoRV FalI FseI FspI FspAI HpaI KasI
MfeI NarI NdeI NheI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacII SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI XbaI XhoI ZraI