Restriction map for
pFN21AE2214
(Linear) (Six-base) MAPSORT of: KIEE2214.seq Check: 9432 from: 1 to: 1590
KIEE2214.orf
With 182 enzymes: SgfI *
July 3, 2012 17:14 ..
AatII G_ACGT'C
Cuts at: 0 977 1590
Size: 977 613
AfeI AGC'GCT
Cuts at: 0 359 871 1345 1590
Size: 359 512 474 245
Fragments arranged by size:
512 474 359 245
AflIII A'CryG_T
Cuts at: 0 338 484 1137 1151 1286 1590
Size: 338 146 653 14 135 304
Fragments arranged by size:
653 338 304 146 135 14
AgeI A'CCGG_T
Cuts at: 0 561 1590
Size: 561 1029
AhdI GACnn_n'nnGTC
Cuts at: 0 678 1590
Size: 678 912
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1306 1590
Size: 1306 284
AlwNI CAG_nnn'CTG
Cuts at: 0 656 1065 1254 1590
Size: 656 409 189 336
Fragments arranged by size:
656 409 336 189
ApaI G_GGCC'C
Cuts at: 0 109 737 1324 1590
Size: 109 628 587 266
Fragments arranged by size:
628 587 266 109
AvaI C'yCGr_G
Cuts at: 0 33 82 109 257 612 861 909 1106
Size: 33 49 27 148 355 249 48 197
Cuts at: 1106 1590
Size: 484
Fragments arranged by size:
484 355 249 197 148 49 48 33
27
BanI G'GyrC_C
Cuts at: 0 66 138 314 381 428 1182 1305 1360
Size: 66 72 176 67 47 754 123 55
Cuts at: 1360 1463 1511 1590
Size: 103 48 79
Fragments arranged by size:
754 176 123 103 79 72 67 66
55 48 47
BanII G_rGCy'C
Cuts at: 0 109 394 590 737 1199 1324 1590
Size: 109 285 196 147 462 125 266
Fragments arranged by size:
462 285 266 196 147 125 109
BbeI G_GCGC'C
Cuts at: 0 70 432 1364 1467 1590
Size: 70 362 932 103 123
Fragments arranged by size:
932 362 123 103 70
BclI T'GATC_A
Cuts at: 0 497 1590
Size: 497 1093
BglI GCCn_nnn'nGGC
Cuts at: 0 104 178 379 1016 1590
Size: 104 74 201 637 574
Fragments arranged by size:
637 574 201 104 74
Bme1580I G_kGCm'C
Cuts at: 0 109 143 384 737 1187 1324 1355 1590
Size: 109 34 241 353 450 137 31 235
Fragments arranged by size:
450 353 241 235 137 109 34 31
BmgBI CAC'GTC
Cuts at: 0 485 1152 1590
Size: 485 667 438
Fragments arranged by size:
667 485 438
BmrI ACTGGGnnnn_n'
Cuts at: 0 1098 1590
Size: 1098 492
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 915 1590
Size: 915 675
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 831 1590
Size: 307 524 759
Fragments arranged by size:
759 524 307
Bpu10I CC'TnA_GC
Cuts at: 0 695 1590
Size: 695 895
BsaAI yAC'GTr
Cuts at: 0 1374 1590
Size: 1374 216
BsaHI Gr'CG_yC
Cuts at: 0 67 151 429 541 761 974 1361 1464
Size: 67 84 278 112 220 213 387 103
Cuts at: 1464 1590
Size: 126
Fragments arranged by size:
387 278 220 213 126 112 103 84
67
BsaWI w'CCGG_w
Cuts at: 0 561 1590
Size: 561 1029
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 40 70 1474 1504 1590
Size: 40 30 1404 30 86
Fragments arranged by size:
1404 86 40 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 218 312 796 826 1386 1590
Size: 218 94 484 30 560 204
Fragments arranged by size:
560 484 218 204 94 30
BseYI C'CCAG_C
Cuts at: 0 44 102 512 872 1297 1317 1540 1590
Size: 44 58 410 360 425 20 223 50
Fragments arranged by size:
425 410 360 223 58 50 44 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1186 1590
Size: 1186 404
BsiEI CG_ry'CG
Cuts at: 0 473 562 675 1161 1428 1590
Size: 473 89 113 486 267 162
Fragments arranged by size:
486 473 267 162 113 89
BsmI GAATG_Cn'
Cuts at: 0 231 1590
Size: 231 1359
Bsp1286I G_dGCh'C
Cuts at: 0 109 143 384 394 590 737 1187 1199
Size: 109 34 241 10 196 147 450 12
Cuts at: 1199 1324 1355 1590
Size: 125 31 235
Fragments arranged by size:
450 241 235 196 147 125 109 34
31 12 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1421 1590
Size: 1421 169
BsrBI CCG'CTC
Cuts at: 0 273 320 349 1587 1590
Size: 273 47 29 1238 3
Fragments arranged by size:
1238 273 47 29 3
BsrFI r'CCGG_y
Cuts at: 0 98 311 475 561 632 1179 1308 1547
Size: 98 213 164 86 71 547 129 239
Cuts at: 1547 1590
Size: 43
Fragments arranged by size:
547 239 213 164 129 98 86 71
43
BsrGI T'GTAC_A
Cuts at: 0 1530 1590
Size: 1530 60
BssSI C'ACGA_G
Cuts at: 0 724 1590
Size: 724 866
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1182 1590
Size: 1182 408
BstYI r'GATC_y
Cuts at: 0 789 1590
Size: 789 801
Bsu36I CC'TnA_GG
Cuts at: 0 1029 1590
Size: 1029 561
BtgI C'CryG_G
Cuts at: 0 220 374 385 448 1145 1356 1436 1590
Size: 220 154 11 63 697 211 80 154
Fragments arranged by size:
697 220 211 154 154 80 63 11
BtsI GCAGTG_nn'
Cuts at: 0 123 773 1590
Size: 123 650 817
Fragments arranged by size:
817 650 123
DraIII CAC_nnn'GTG
Cuts at: 0 1209 1590
Size: 1209 381
DrdI GACnn_nn'nnGTC
Cuts at: 0 973 1590
Size: 973 617
EaeI y'GGCC_r
Cuts at: 0 157 470 1158 1571 1590
Size: 157 313 688 413 19
Fragments arranged by size:
688 413 313 157 19
EagI C'GGCC_G
Cuts at: 0 470 1158 1590
Size: 470 688 432
Fragments arranged by size:
688 470 432
EarI CTCTTCn'nnn_
Cuts at: 0 943 1590
Size: 943 647
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 76 979 1590
Size: 76 903 611
Fragments arranged by size:
903 611 76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 831 1590
Size: 307 524 759
Fragments arranged by size:
759 524 307
EcoNI CCTnn'n_nnAGG
Cuts at: 0 727 1590
Size: 727 863
EcoO109I rG'GnC_Cy
Cuts at: 0 86 106 733 734 857 1590
Size: 86 20 627 1 123 733
Fragments arranged by size:
733 627 123 86 20 1
HaeII r_GCGC'y
Cuts at: 0 9 70 361 432 496 611 873 1101
Size: 9 61 291 71 64 115 262 228
Cuts at: 1101 1347 1364 1467 1590
Size: 246 17 103 123
Fragments arranged by size:
291 262 246 228 123 115 103 71
64 61 17 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 664 696 1012 1044 1590
Size: 664 32 316 32 546
Fragments arranged by size:
664 546 316 32 32
KasI G'GCGC_C
Cuts at: 0 66 428 1360 1463 1590
Size: 66 362 932 103 127
Fragments arranged by size:
932 362 127 103 66
MluI A'CGCG_T
Cuts at: 0 338 1286 1590
Size: 338 948 304
Fragments arranged by size:
948 338 304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 1590
Size: 782 808
MscI TGG'CCA
Cuts at: 0 159 1573 1590
Size: 159 1414 17
Fragments arranged by size:
1414 159 17
MslI CAynn'nnrTG
Cuts at: 0 1372 1590
Size: 1372 218
MspA1I CmG'CkG
Cuts at: 0 222 266 522 531 653 820 1358 1438
Size: 222 44 256 9 122 167 538 80
Cuts at: 1438 1577 1590
Size: 139 13
Fragments arranged by size:
538 256 222 167 139 122 80 44
13 9
NaeI GCC'GGC
Cuts at: 0 100 477 634 1590
Size: 100 377 157 956
Fragments arranged by size:
956 377 157 100
NarI GG'CG_CC
Cuts at: 0 67 429 1361 1464 1590
Size: 67 362 932 103 126
Fragments arranged by size:
932 362 126 103 67
NcoI C'CATG_G
Cuts at: 0 374 448 1590
Size: 374 74 1142
Fragments arranged by size:
1142 374 74
NgoMIV G'CCGG_C
Cuts at: 0 98 475 632 1590
Size: 98 377 157 958
Fragments arranged by size:
958 377 157 98
NotI GC'GGCC_GC
Cuts at: 0 470 1590
Size: 470 1120
NruI TCG'CGA
Cuts at: 0 558 1590
Size: 558 1032
NspI r_CATG'y
Cuts at: 0 1141 1590
Size: 1141 449
PasI CC'CwG_GG
Cuts at: 0 536 755 1590
Size: 536 219 835
Fragments arranged by size:
835 536 219
PciI A'CATG_T
Cuts at: 0 1137 1590
Size: 1137 453
PfoI T'CCnGG_A
Cuts at: 0 1051 1590
Size: 1051 539
PmlI CAC'GTG
Cuts at: 0 1374 1590
Size: 1374 216
PspOMI G'GGCC_C
Cuts at: 0 105 733 1320 1590
Size: 105 628 587 270
Fragments arranged by size:
628 587 270 105
PvuII CAG'CTG
Cuts at: 0 522 1577 1590
Size: 522 1055 13
Fragments arranged by size:
1055 522 13
SacII CC_GC'GG
Cuts at: 0 223 1359 1439 1590
Size: 223 1136 80 151
Fragments arranged by size:
1136 223 151 80
SfcI C'TryA_G
Cuts at: 0 298 1590
Size: 298 1292
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 104 1590
Size: 104 1486
SfoI GGC'GCC
Cuts at: 0 68 430 1362 1465 1590
Size: 68 362 932 103 125
Fragments arranged by size:
932 362 125 103 68
SmaI CCC'GGG
Cuts at: 0 84 111 259 863 911 1108 1590
Size: 84 27 148 604 48 197 482
Fragments arranged by size:
604 482 197 148 84 48 27
StuI AGG'CCT
Cuts at: 0 1034 1590
Size: 1034 556
StyI C'CwwG_G
Cuts at: 0 374 448 1507 1590
Size: 374 74 1059 83
Fragments arranged by size:
1059 374 83 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1065 1590
Size: 1065 525
TatI w'GTAC_w
Cuts at: 0 1530 1590
Size: 1530 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 40 72 122 154 444 476 1474 1506
Size: 40 32 50 32 290 32 998 32
Cuts at: 1506 1590
Size: 84
Fragments arranged by size:
998 290 84 50 40 32 32 32
32
XmaI C'CCGG_G
Cuts at: 0 82 109 257 861 909 1106 1590
Size: 82 27 148 604 48 197 484
Fragments arranged by size:
604 484 197 148 82 48 27
ZraI GAC'GTC
Cuts at: 0 975 1590
Size: 975 615
Enzymes that do cut:
AatII AfeI AflIII AgeI AhdI AlfI AlwNI ApaI
AvaI BanI BanII BbeI BclI BglI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsmI Bsp1286I BspMI BsrBI
BsrFI BsrGI BssSI BstAPI BstYI Bsu36I BtgI BtsI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NruI NspI
PasI PciI PfoI PmlI PspOMI PvuII SacII SfcI
SfiI SfoI SmaI StuI StyI TaqII TatI TstI
XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AflII AleI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BcgI BciVI BfrBI BglII BlpI BmtI
BpuEI BsaI BsaBI BsiHKAI BsiWI BsmBI BspEI BspHI
BsrDI BssHII BstBI BstEII BstXI BstZ17I BtgZI ClaI
CspCI DraI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI NdeI NheI
NsiI PacI PflMI PmeI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI
XhoI XmnI