Restriction map for
pFN21AE2213
(Linear) (Six-base) MAPSORT of: KIEE2213.seq Check: 1749 from: 1 to: 1119
KIEE2213.orf
With 182 enzymes: SgfI *
March 22, 2012 15:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 142 884 1119
Size: 142 742 235
Fragments arranged by size:
742 235 142
AatII G_ACGT'C
Cuts at: 0 301 1119
Size: 301 818
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 337 437 1044 1119
Size: 229 108 100 607 75
Fragments arranged by size:
607 229 108 100 75
AgeI A'CCGG_T
Cuts at: 0 341 1119
Size: 341 778
AleI CACnn'nnGTG
Cuts at: 0 546 1119
Size: 546 573
AlwNI CAG_nnn'CTG
Cuts at: 0 148 393 1119
Size: 148 245 726
Fragments arranged by size:
726 245 148
ApaI G_GGCC'C
Cuts at: 0 128 1119
Size: 128 991
BamHI G'GATC_C
Cuts at: 0 4 1119
Size: 4 1115
BanII G_rGCy'C
Cuts at: 0 128 185 660 1119
Size: 128 57 475 459
Fragments arranged by size:
475 459 128 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 953 1119
Size: 953 166
BfrBI ATG'CAT
Cuts at: 0 674 1119
Size: 674 445
Bme1580I G_kGCm'C
Cuts at: 0 128 1119
Size: 128 991
BmgBI CAC'GTC
Cuts at: 0 135 1119
Size: 135 984
BmrI ACTGGGnnnn_n'
Cuts at: 0 861 1119
Size: 861 258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 510 674 901 1119
Size: 415 95 164 227 218
Fragments arranged by size:
415 227 218 164 95
Bpu10I CC'TnA_GC
Cuts at: 0 665 1119
Size: 665 454
BsaHI Gr'CG_yC
Cuts at: 0 298 365 1119
Size: 298 67 754
Fragments arranged by size:
754 298 67
BsaWI w'CCGG_w
Cuts at: 0 341 810 1119
Size: 341 469 309
Fragments arranged by size:
469 341 309
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1033 1119
Size: 1033 86
BseYI C'CCAG_C
Cuts at: 0 90 347 1119
Size: 90 257 772
Fragments arranged by size:
772 257 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1018 1119
Size: 1018 101
BsiHKAI G_wGCw'C
Cuts at: 0 185 660 871 1119
Size: 185 475 211 248
Fragments arranged by size:
475 248 211 185
BsmI GAATG_Cn'
Cuts at: 0 676 917 1119
Size: 676 241 202
Fragments arranged by size:
676 241 202
BsmBI CGTCTCn'nnnn_
Cuts at: 0 220 391 1119
Size: 220 171 728
Fragments arranged by size:
728 220 171
Bsp1286I G_dGCh'C
Cuts at: 0 128 185 660 871 1119
Size: 128 57 475 211 248
Fragments arranged by size:
475 248 211 128 57
BspEI T'CCGG_A
Cuts at: 0 810 1119
Size: 810 309
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 142 884 1119
Size: 142 742 235
Fragments arranged by size:
742 235 142
BsrBI CCG'CTC
Cuts at: 0 456 1119
Size: 456 663
BsrFI r'CCGG_y
Cuts at: 0 341 1119
Size: 341 778
BsrGI T'GTAC_A
Cuts at: 0 824 1119
Size: 824 295
BstAPI GCAn_nnn'nTGC
Cuts at: 0 148 477 1119
Size: 148 329 642
Fragments arranged by size:
642 329 148
BstYI r'GATC_y
Cuts at: 0 4 887 1119
Size: 4 883 232
Fragments arranged by size:
883 232 4
BtgI C'CryG_G
Cuts at: 0 171 284 1119
Size: 171 113 835
Fragments arranged by size:
835 171 113
BtsI GCAGTG_nn'
Cuts at: 0 149 1119
Size: 149 970
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 891 926 1119
Size: 891 35 193
Fragments arranged by size:
891 193 35
DraIII CAC_nnn'GTG
Cuts at: 0 630 1119
Size: 630 489
EaeI y'GGCC_r
Cuts at: 0 431 833 1119
Size: 431 402 286
Fragments arranged by size:
431 402 286
EarI CTCTTCn'nnn_
Cuts at: 0 79 413 812 1053 1119
Size: 79 334 399 241 66
Fragments arranged by size:
399 334 241 79 66
EcoICRI GAG'CTC
Cuts at: 0 183 658 1119
Size: 183 475 461
Fragments arranged by size:
475 461 183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 337 415 437 510 674 901 1044
Size: 229 108 78 22 73 164 227 143
Cuts at: 1044 1119
Size: 75
Fragments arranged by size:
229 227 164 143 108 78 75 73
22
EcoO109I rG'GnC_Cy
Cuts at: 0 117 124 125 840 958 1019 1119
Size: 117 7 1 715 118 61 100
Fragments arranged by size:
715 118 117 100 61 7 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 762 823 1119
Size: 762 61 296
Fragments arranged by size:
762 296 61
HaeII r_GCGC'y
Cuts at: 0 170 1119
Size: 170 949
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 683 715 1119
Size: 683 32 404
Fragments arranged by size:
683 404 32
HincII GTy'rAC
Cuts at: 0 498 1119
Size: 498 621
HindIII A'AGCT_T
Cuts at: 0 242 1119
Size: 242 877
MscI TGG'CCA
Cuts at: 0 835 1119
Size: 835 284
MslI CAynn'nnrTG
Cuts at: 0 199 546 994 1119
Size: 199 347 448 125
Fragments arranged by size:
448 347 199 125
MspA1I CmG'CkG
Cuts at: 0 415 1119
Size: 415 704
NcoI C'CATG_G
Cuts at: 0 171 1119
Size: 171 948
NsiI A_TGCA'T
Cuts at: 0 676 1119
Size: 676 443
NspI r_CATG'y
Cuts at: 0 315 678 993 1041 1119
Size: 315 363 315 48 78
Fragments arranged by size:
363 315 315 78 48
PasI CC'CwG_GG
Cuts at: 0 121 1119
Size: 121 998
PflMI CCAn_nnn'nTGG
Cuts at: 0 393 913 1119
Size: 393 520 206
Fragments arranged by size:
520 393 206
PfoI T'CCnGG_A
Cuts at: 0 755 779 938 1119
Size: 755 24 159 181
Fragments arranged by size:
755 181 159 24
PpuMI rG'GwC_Cy
Cuts at: 0 840 958 1019 1119
Size: 840 118 61 100
Fragments arranged by size:
840 118 100 61
PshAI GACnn'nnGTC
Cuts at: 0 286 1119
Size: 286 833
PspOMI G'GGCC_C
Cuts at: 0 124 1119
Size: 124 995
PstI C_TGCA'G
Cuts at: 0 153 482 1119
Size: 153 329 637
Fragments arranged by size:
637 329 153
SacI G_AGCT'C
Cuts at: 0 185 660 1119
Size: 185 475 459
Fragments arranged by size:
475 459 185
SapI GCTCTTCn'nnn_
Cuts at: 0 413 1119
Size: 413 706
SfcI C'TryA_G
Cuts at: 0 149 438 460 478 1119
Size: 149 289 22 18 641
Fragments arranged by size:
641 289 149 22 18
SphI G_CATG'C
Cuts at: 0 315 1119
Size: 315 804
StuI AGG'CCT
Cuts at: 0 22 1119
Size: 22 1097
StyI C'CwwG_G
Cuts at: 0 171 555 1119
Size: 171 384 564
Fragments arranged by size:
564 384 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1042 1119
Size: 1042 77
TatI w'GTAC_w
Cuts at: 0 320 824 1119
Size: 320 504 295
Fragments arranged by size:
504 320 295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 54 725 1119
Size: 54 671 394
Fragments arranged by size:
671 394 54
ZraI GAC'GTC
Cuts at: 0 299 1119
Size: 299 820
Enzymes that do cut:
AarI AatII AcuI AgeI AleI AlwNI ApaI BamHI
BanII BbsI BfrBI Bme1580I BmgBI BmrI BpmI Bpu10I
BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BstAPI BstYI
BtgI BtsI CspCI DraIII EaeI EarI EcoICRI Eco57MI
EcoO109I FalI HaeII Hin4I HincII HindIII MscI MslI
MspA1I NcoI NsiI NspI PasI PflMI PfoI PpuMI
PshAI PspOMI PstI SacI SapI SfcI SphI StuI
StyI TaqII TatI XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BanI BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaXI BsiEI BsiWI BspHI BsrDI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraI DrdI
EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PciI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI
SwaI TstI Tth111I XbaI XhoI XmaI XmnI