(Linear) (Six-base) MAPSORT of: KIEE2211.seq  Check: 4130  from: 1  to: 336



KIEE2211.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:55  ..

ApoI r'AATT_y

Cuts at:      0      99     336 
   Size:         99     237
 
AvaI C'yCGr_G

Cuts at:      0     269     336 
   Size:        269      67
 
BanII G_rGCy'C

Cuts at:      0     142     336 
   Size:        142     194
 
BbvCI CC'TCA_GC

Cuts at:      0     144     336 
   Size:        144     192
 
BglI GCCn_nnn'nGGC

Cuts at:      0     203     336 
   Size:        203     133
 
BglII A'GATC_T

Cuts at:      0     251     336 
   Size:        251      85
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      55     336 
   Size:         55     281
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     159     336 
   Size:        159     177
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     336 
   Size:        280      56
 
Bpu10I CC'TnA_GC

Cuts at:      0     144     336 
   Size:        144     192
 
BsiHKAI G_wGCw'C

Cuts at:      0      15     336 
   Size:         15     321
 
Bsp1286I G_dGCh'C

Cuts at:      0      15     142     336 
   Size:         15     127     194

  Fragments arranged by size: 

                 194     127      15
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     132     336 
   Size:        132     204
 
BstYI r'GATC_y

Cuts at:      0     251     336 
   Size:        251      85
 
BtgI C'CryG_G

Cuts at:      0     163     336 
   Size:        163     173
 
BtsI GCAGTG_nn'

Cuts at:      0     180     330     336 
   Size:        180     150       6

  Fragments arranged by size: 

                 180     150       6
 
EaeI y'GGCC_r

Cuts at:      0     160     327     336 
   Size:        160     167       9

  Fragments arranged by size: 

                 167     160       9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      79     336 
   Size:         79     257
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     336 
   Size:        280      56
 
EcoRI G'AATT_C

Cuts at:      0      99     336 
   Size:         99     237
 
MslI CAynn'nnrTG

Cuts at:      0     162     336 
   Size:        162     174
 
MspA1I CmG'CkG

Cuts at:      0     148     336 
   Size:        148     188
 
PstI C_TGCA'G

Cuts at:      0     187     336 
   Size:        187     149
 
PvuII CAG'CTG

Cuts at:      0     148     336 
   Size:        148     188
 
SexAI A'CCwGG_T

Cuts at:      0       4     336 
   Size:          4     332
 
SfcI C'TryA_G

Cuts at:      0     183     336 
   Size:        183     153
 
SmaI CCC'GGG

Cuts at:      0     271     336 
   Size:        271      65
 
SrfI GCCC'GGGC

Cuts at:      0     271     336 
   Size:        271      65
 
XmaI C'CCGG_G

Cuts at:      0     269     336 
   Size:        269      67
 

 Enzymes that do cut:

     ApoI     AvaI    BanII    BbvCI     BglI    BglII     BmrI     BplI
     BpmI   Bpu10I  BsiHKAI Bsp1286I    BspMI    BstYI     BtgI     BtsI
     EaeI     EciI  Eco57MI    EcoRI     MslI   MspA1I     PstI    PvuII
    SexAI     SfcI     SmaI     SrfI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BlpI Bme1580I
    BmgBI     BmtI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI