Restriction map for
pFN21AE2205
(Linear) (Six-base) MAPSORT of: KIEE2205.seq Check: 1208 from: 1 to: 1773
KIEE2205.orf
With 182 enzymes: SgfI *
July 15, 2010 18:55 ..
AccI GT'mk_AC
Cuts at: 0 488 1773
Size: 488 1285
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 1210 1342 1639 1773
Size: 103 1107 132 297 134
Fragments arranged by size:
1107 297 134 132 103
AflIII A'CryG_T
Cuts at: 0 1572 1773
Size: 1572 201
AleI CACnn'nnGTG
Cuts at: 0 376 1773
Size: 376 1397
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1114 1146 1773
Size: 1114 32 627
Fragments arranged by size:
1114 627 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1050 1311 1320 1495 1773
Size: 1050 261 9 175 278
Fragments arranged by size:
1050 278 261 175 9
ApaI G_GGCC'C
Cuts at: 0 28 1152 1614 1773
Size: 28 1124 462 159
Fragments arranged by size:
1124 462 159 28
ApoI r'AATT_y
Cuts at: 0 796 1188 1773
Size: 796 392 585
Fragments arranged by size:
796 585 392
BanI G'GyrC_C
Cuts at: 0 1341 1773
Size: 1341 432
BanII G_rGCy'C
Cuts at: 0 28 1152 1614 1773
Size: 28 1124 462 159
Fragments arranged by size:
1124 462 159 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 696 1086 1642 1773
Size: 696 390 556 131
Fragments arranged by size:
696 556 390 131
BbvCI CC'TCA_GC
Cuts at: 0 814 1773
Size: 814 959
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 357 391 1773
Size: 357 34 1382
Fragments arranged by size:
1382 357 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1349 1773
Size: 1349 424
BclI T'GATC_A
Cuts at: 0 1428 1773
Size: 1428 345
Bme1580I G_kGCm'C
Cuts at: 0 28 1152 1614 1773
Size: 28 1124 462 159
Fragments arranged by size:
1124 462 159 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 1146 1608 1773
Size: 1146 462 165
Fragments arranged by size:
1146 462 165
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 351 1773
Size: 351 1422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1027 1773
Size: 1027 746
Bpu10I CC'TnA_GC
Cuts at: 0 364 814 1773
Size: 364 450 959
Fragments arranged by size:
959 450 364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1660 1773
Size: 1660 113
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 456 1549 1773
Size: 456 1093 224
Fragments arranged by size:
1093 456 224
BseYI C'CCAG_C
Cuts at: 0 175 1773
Size: 175 1598
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1042 1045 1773
Size: 1042 3 728
Fragments arranged by size:
1042 728 3
BsmI GAATG_Cn'
Cuts at: 0 848 1773
Size: 848 925
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1300 1773
Size: 1300 473
Bsp1286I G_dGCh'C
Cuts at: 0 28 1152 1614 1773
Size: 28 1124 462 159
Fragments arranged by size:
1124 462 159 28
BspHI T'CATG_A
Cuts at: 0 1097 1773
Size: 1097 676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1067 1773
Size: 1067 706
BsrBI CCG'CTC
Cuts at: 0 718 1773
Size: 718 1055
BsrGI T'GTAC_A
Cuts at: 0 380 1773
Size: 380 1393
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1495 1773
Size: 1495 278
BstEII G'GTnAC_C
Cuts at: 0 1288 1773
Size: 1288 485
BstYI r'GATC_y
Cuts at: 0 86 341 915 1773
Size: 86 255 574 858
Fragments arranged by size:
858 574 255 86
BtgI C'CryG_G
Cuts at: 0 413 1031 1773
Size: 413 618 742
Fragments arranged by size:
742 618 413
BtsI GCAGTG_nn'
Cuts at: 0 581 915 980 1773
Size: 581 334 65 793
Fragments arranged by size:
793 581 334 65
DraIII CAC_nnn'GTG
Cuts at: 0 432 1773
Size: 432 1341
DrdI GACnn_nn'nnGTC
Cuts at: 0 1431 1773
Size: 1431 342
EaeI y'GGCC_r
Cuts at: 0 353 1773
Size: 353 1420
EarI CTCTTCn'nnn_
Cuts at: 0 755 1552 1773
Size: 755 797 221
Fragments arranged by size:
797 755 221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 1027 1210 1342 1639 1773
Size: 103 924 183 132 297 134
Fragments arranged by size:
924 297 183 134 132 103
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1675 1773
Size: 1675 98
EcoO109I rG'GnC_Cy
Cuts at: 0 297 1148 1610 1773
Size: 297 851 462 163
Fragments arranged by size:
851 462 297 163
EcoRI G'AATT_C
Cuts at: 0 1188 1773
Size: 1188 585
EcoRV GAT'ATC
Cuts at: 0 1728 1773
Size: 1728 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 563 595 1629 1661 1773
Size: 563 32 1034 32 112
Fragments arranged by size:
1034 563 112 32 32
HindIII A'AGCT_T
Cuts at: 0 645 1773
Size: 645 1128
MfeI C'AATT_G
Cuts at: 0 1582 1773
Size: 1582 191
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1136 1490 1773
Size: 1136 354 283
Fragments arranged by size:
1136 354 283
MscI TGG'CCA
Cuts at: 0 355 1773
Size: 355 1418
MslI CAynn'nnrTG
Cuts at: 0 376 528 824 1773
Size: 376 152 296 949
Fragments arranged by size:
949 376 296 152
MspA1I CmG'CkG
Cuts at: 0 60 499 1026 1773
Size: 60 439 527 747
Fragments arranged by size:
747 527 439 60
NcoI C'CATG_G
Cuts at: 0 413 1773
Size: 413 1360
NdeI CA'TA_TG
Cuts at: 0 1720 1773
Size: 1720 53
NspI r_CATG'y
Cuts at: 0 1576 1773
Size: 1576 197
PciI A'CATG_T
Cuts at: 0 1572 1773
Size: 1572 201
PflMI CCAn_nnn'nTGG
Cuts at: 0 859 1620 1773
Size: 859 761 153
Fragments arranged by size:
859 761 153
PfoI T'CCnGG_A
Cuts at: 0 337 1043 1773
Size: 337 706 730
Fragments arranged by size:
730 706 337
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1658 1690 1773
Size: 1658 32 83
Fragments arranged by size:
1658 83 32
PshAI GACnn'nnGTC
Cuts at: 0 892 1773
Size: 892 881
PspOMI G'GGCC_C
Cuts at: 0 24 1148 1610 1773
Size: 24 1124 462 163
Fragments arranged by size:
1124 462 163 24
PstI C_TGCA'G
Cuts at: 0 1316 1491 1773
Size: 1316 175 282
Fragments arranged by size:
1316 282 175
PvuII CAG'CTG
Cuts at: 0 60 499 1026 1773
Size: 60 439 527 747
Fragments arranged by size:
747 527 439 60
SapI GCTCTTCn'nnn_
Cuts at: 0 755 1773
Size: 755 1018
ScaI AGT'ACT
Cuts at: 0 1333 1773
Size: 1333 440
SexAI A'CCwGG_T
Cuts at: 0 471 1337 1773
Size: 471 866 436
Fragments arranged by size:
866 471 436
SfcI C'TryA_G
Cuts at: 0 485 1312 1420 1487 1773
Size: 485 827 108 67 286
Fragments arranged by size:
827 485 286 108 67
SmlI C'TyrA_G
Cuts at: 0 1675 1773
Size: 1675 98
SspI AAT'ATT
Cuts at: 0 1384 1773
Size: 1384 389
StyI C'CwwG_G
Cuts at: 0 224 350 413 947 1345 1394 1773
Size: 224 126 63 534 398 49 379
Fragments arranged by size:
534 398 379 224 126 63 49
TatI w'GTAC_w
Cuts at: 0 380 1331 1773
Size: 380 951 442
Fragments arranged by size:
951 442 380
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 228 260 1773
Size: 228 32 1513
Fragments arranged by size:
1513 228 32
XbaI T'CTAG_A
Cuts at: 0 870 1773
Size: 870 903
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 527 1401 1773
Size: 527 874 372
Fragments arranged by size:
874 527 372
XmnI GAAnn'nnTTC
Cuts at: 0 697 1108 1272 1670 1773
Size: 697 411 164 398 103
Fragments arranged by size:
697 411 398 164 103
Enzymes that do cut:
AccI AcuI AflIII AleI AloI AlwNI ApaI ApoI
BanI BanII BbsI BbvCI BcgI BciVI BclI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BseRI BseYI BsgI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrGI BstAPI
BstEII BstYI BtgI BtsI DraIII DrdI EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI EcoRV Hin4I HindIII MfeI
MmeI MscI MslI MspA1I NcoI NdeI NspI PciI
PflMI PfoI PpiI PshAI PspOMI PstI PvuII SapI
ScaI SexAI SfcI SmlI SspI StyI TatI TstI
XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BfrBI BglI BglII BlpI BmgBI BmtI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI
BsiWI BspEI BsrDI BsrFI BssHII BssSI BstBI BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI EagI EciI
EcoICRI FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MluI NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PasI PmeI PmlI PpuMI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TaqII Tth111I XhoI XmaI
ZraI