(Linear) (Six-base) MAPSORT of: KIEE2194.seq  Check: 834  from: 1  to: 765



KIEE2194.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:55  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     680     765 
   Size:        680      85
 
AfeI AGC'GCT

Cuts at:      0     737     765 
   Size:        737      28
 
ApoI r'AATT_y

Cuts at:      0     400     548     765 
   Size:        400     148     217

  Fragments arranged by size: 

                 400     217     148
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     233     758     765 
   Size:        233     525       7

  Fragments arranged by size: 

                 525     233       7
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     487     521     765 
   Size:        487      34     244

  Fragments arranged by size: 

                 487     244      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     612     765 
   Size:        612     153
 
BglI GCCn_nnn'nGGC

Cuts at:      0      46     765 
   Size:         46     719
 
BlpI GC'TnA_GC

Cuts at:      0      50     765 
   Size:         50     715
 
BmgBI CAC'GTC

Cuts at:      0     747     765 
   Size:        747      18
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     765 
   Size:        198     567
 
Bpu10I CC'TnA_GC

Cuts at:      0     510     765 
   Size:        510     255
 
BsaAI yAC'GTr

Cuts at:      0     673     765 
   Size:        673      92
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     236     765 
   Size:        236     529
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     566     765 
   Size:        566     199
 
BsrDI GCAATG_nn'

Cuts at:      0     482     765 
   Size:        482     283
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     403     765 
   Size:        403     362
 
BstYI r'GATC_y

Cuts at:      0      15     765 
   Size:         15     750
 
EaeI y'GGCC_r

Cuts at:      0      38     765 
   Size:         38     727
 
EarI CTCTTCn'nnn_

Cuts at:      0     162     713     765 
   Size:        162     551      52

  Fragments arranged by size: 

                 551     162      52
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     680     765 
   Size:        198     482      85

  Fragments arranged by size: 

                 482     198      85
 
HaeII r_GCGC'y

Cuts at:      0     739     765 
   Size:        739      26
 
MfeI C'AATT_G

Cuts at:      0     605     765 
   Size:        605     160
 
NspI r_CATG'y

Cuts at:      0     705     765 
   Size:        705      60
 
PfoI T'CCnGG_A

Cuts at:      0     175     765 
   Size:        175     590
 
PsiI TTA'TAA

Cuts at:      0     365     455     765 
   Size:        365      90     310

  Fragments arranged by size: 

                 365     310      90
 
PstI C_TGCA'G

Cuts at:      0     577     743     765 
   Size:        577     166      22

  Fragments arranged by size: 

                 577     166      22
 
SapI GCTCTTCn'nnn_

Cuts at:      0     162     713     765 
   Size:        162     551      52

  Fragments arranged by size: 

                 551     162      52
 
SbfI CC_TGCA'GG

Cuts at:      0     577     765 
   Size:        577     188
 
SfcI C'TryA_G

Cuts at:      0     573     739     765 
   Size:        573     166      26

  Fragments arranged by size: 

                 573     166      26
 
StyI C'CwwG_G

Cuts at:      0     350     765 
   Size:        350     415
 
TatI w'GTAC_w

Cuts at:      0     306     765 
   Size:        306     459
 

 Enzymes that do cut:

     AcuI     AfeI     ApoI     BbsI     BcgI    BciVI     BglI     BlpI
    BmgBI     BpmI   Bpu10I    BsaAI    BseRI    BspMI    BsrDI   BstAPI
    BstYI     EaeI     EarI  Eco57MI    HaeII     MfeI     NspI     PfoI
     PsiI     PstI     SapI     SbfI     SfcI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BclI    BfrBI    BglII Bme1580I     BmrI     BmtI
     BplI    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI