Restriction map for
pFN21AE2190
(Linear) (Six-base) MAPSORT of: KIEE2190.seq Check: 6915 from: 1 to: 1593
KIEE2190.orf
With 182 enzymes: SgfI *
July 15, 2010 18:55 ..
AccI GT'mk_AC
Cuts at: 0 1283 1593
Size: 1283 310
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 542 1453 1593
Size: 337 205 911 140
Fragments arranged by size:
911 337 205 140
AfeI AGC'GCT
Cuts at: 0 333 1593
Size: 333 1260
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1364 1593
Size: 1364 229
AlwNI CAG_nnn'CTG
Cuts at: 0 238 823 1149 1593
Size: 238 585 326 444
Fragments arranged by size:
585 444 326 238
ApaLI G'TGCA_C
Cuts at: 0 199 788 1593
Size: 199 589 805
Fragments arranged by size:
805 589 199
ApoI r'AATT_y
Cuts at: 0 729 1153 1593
Size: 729 424 440
Fragments arranged by size:
729 440 424
AvaI C'yCGr_G
Cuts at: 0 662 1593
Size: 662 931
BamHI G'GATC_C
Cuts at: 0 828 1455 1593
Size: 828 627 138
Fragments arranged by size:
828 627 138
BbsI GAAGACnn'nnnn_
Cuts at: 0 115 267 343 997 1593
Size: 115 152 76 654 596
Fragments arranged by size:
654 596 152 115 76
BbvCI CC'TCA_GC
Cuts at: 0 647 1593
Size: 647 946
BglII A'GATC_T
Cuts at: 0 276 580 1593
Size: 276 304 1013
Fragments arranged by size:
1013 304 276
BlpI GC'TnA_GC
Cuts at: 0 13 1593
Size: 13 1580
Bme1580I G_kGCm'C
Cuts at: 0 203 792 1249 1593
Size: 203 589 457 344
Fragments arranged by size:
589 457 344 203
BmgBI CAC'GTC
Cuts at: 0 9 229 1593
Size: 9 220 1364
Fragments arranged by size:
1364 220 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 1323 1593
Size: 1323 270
Bpu10I CC'TnA_GC
Cuts at: 0 647 1593
Size: 647 946
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 1019 1292 1593
Size: 350 669 273 301
Fragments arranged by size:
669 350 301 273
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 56 86 984 1014 1593
Size: 56 30 898 30 579
Fragments arranged by size:
898 579 56 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 645 1593
Size: 645 948
BseYI C'CCAG_C
Cuts at: 0 858 1010 1593
Size: 858 152 583
Fragments arranged by size:
858 583 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 1360 1593
Size: 251 1109 233
Fragments arranged by size:
1109 251 233
BsiHKAI G_wGCw'C
Cuts at: 0 203 792 1593
Size: 203 589 801
Fragments arranged by size:
801 589 203
BsmBI CGTCTCn'nnnn_
Cuts at: 0 59 1593
Size: 59 1534
Bsp1286I G_dGCh'C
Cuts at: 0 203 792 1249 1593
Size: 203 589 457 344
Fragments arranged by size:
589 457 344 203
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 813 1593
Size: 813 780
BsrGI T'GTAC_A
Cuts at: 0 492 1593
Size: 492 1101
BstXI CCAn_nnnn'nTGG
Cuts at: 0 567 1593
Size: 567 1026
BstYI r'GATC_y
Cuts at: 0 276 580 828 1066 1455 1593
Size: 276 304 248 238 389 138
Fragments arranged by size:
389 304 276 248 238 138
BtgI C'CryG_G
Cuts at: 0 945 1372 1593
Size: 945 427 221
Fragments arranged by size:
945 427 221
BtsI GCAGTG_nn'
Cuts at: 0 1247 1593
Size: 1247 346
EaeI y'GGCC_r
Cuts at: 0 1369 1593
Size: 1369 224
EarI CTCTTCn'nnn_
Cuts at: 0 422 1593
Size: 422 1171
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1593
Size: 19 1574
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 542 1453 1593
Size: 337 205 911 140
Fragments arranged by size:
911 337 205 140
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1141 1593
Size: 1141 452
EcoO109I rG'GnC_Cy
Cuts at: 0 1146 1345 1593
Size: 1146 199 248
Fragments arranged by size:
1146 248 199
EcoRV GAT'ATC
Cuts at: 0 289 1593
Size: 289 1304
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 298 1593
Size: 298 1295
FspI TGC'GCA
Cuts at: 0 841 1593
Size: 841 752
HaeII r_GCGC'y
Cuts at: 0 335 1593
Size: 335 1258
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 572 604 656 688 964 984 996 1016
Size: 572 32 52 32 276 20 12 20
Cuts at: 1016 1593
Size: 577
Fragments arranged by size:
577 572 276 52 32 32 20 20
12
HincII GTy'rAC
Cuts at: 0 308 1192 1593
Size: 308 884 401
Fragments arranged by size:
884 401 308
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 1593
Size: 249 1344
MscI TGG'CCA
Cuts at: 0 1371 1593
Size: 1371 222
MspA1I CmG'CkG
Cuts at: 0 862 1593
Size: 862 731
NcoI C'CATG_G
Cuts at: 0 945 1372 1593
Size: 945 427 221
Fragments arranged by size:
945 427 221
PpuMI rG'GwC_Cy
Cuts at: 0 1146 1345 1593
Size: 1146 199 248
Fragments arranged by size:
1146 248 199
PstI C_TGCA'G
Cuts at: 0 384 1008 1098 1593
Size: 384 624 90 495
Fragments arranged by size:
624 495 384 90
PvuII CAG'CTG
Cuts at: 0 862 1593
Size: 862 731
ScaI AGT'ACT
Cuts at: 0 160 1593
Size: 160 1433
SfcI C'TryA_G
Cuts at: 0 380 1004 1094 1141 1593
Size: 380 624 90 47 452
Fragments arranged by size:
624 452 380 90 47
SmlI C'TyrA_G
Cuts at: 0 365 1034 1307 1584 1593
Size: 365 669 273 277 9
Fragments arranged by size:
669 365 277 273 9
SspI AAT'ATT
Cuts at: 0 246 1593
Size: 246 1347
StyI C'CwwG_G
Cuts at: 0 945 1372 1593
Size: 945 427 221
Fragments arranged by size:
945 427 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1092 1593
Size: 1092 501
TatI w'GTAC_w
Cuts at: 0 29 158 492 1593
Size: 29 129 334 1101
Fragments arranged by size:
1101 334 129 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 56 88 415 447 1593
Size: 56 32 327 32 1146
Fragments arranged by size:
1146 327 56 32 32
XbaI T'CTAG_A
Cuts at: 0 600 1593
Size: 600 993
XmnI GAAnn'nnTTC
Cuts at: 0 245 1593
Size: 245 1348
Enzymes that do cut:
AccI AcuI AfeI AlfI AlwNI ApaLI ApoI AvaI
BamHI BbsI BbvCI BglII BlpI Bme1580I BmgBI BmrI
Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BsrGI BstXI BstYI BtgI BtsI EaeI
EarI EciI Eco57MI EcoNI EcoO109I EcoRV FalI FspI
HaeII Hin4I HincII MmeI MscI MspA1I NcoI PpuMI
PstI PvuII ScaI SfcI SmlI SspI StyI TaqII
TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI ApaI AscI AseI AsiSI AvrII BaeI
BanI BanII BbeI BcgI BciVI BclI BfrBI BglI
BmtI BplI BpmI BsaI BsaAI BsaBI BsaHI BsaWI
BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI
FseI FspAI HindIII HpaI KasI KpnI MfeI MluI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI Tth111I XcmI XhoI XmaI ZraI