Restriction map for
pFN21AE2187
(Linear) (Six-base) MAPSORT of: KIEE2187.seq Check: 260 from: 1 to: 1422
KIEE2187.orf
With 182 enzymes: SgfI *
July 15, 2010 18:55 ..
AccI GT'mk_AC
Cuts at: 0 85 957 1422
Size: 85 872 465
Fragments arranged by size:
872 465 85
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 265 835 1422
Size: 238 27 570 587
Fragments arranged by size:
587 570 238 27
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 274 1422
Size: 274 1148
AlwNI CAG_nnn'CTG
Cuts at: 0 870 1422
Size: 870 552
ApoI r'AATT_y
Cuts at: 0 328 356 415 502 1264 1422
Size: 328 28 59 87 762 158
Fragments arranged by size:
762 328 158 87 59 28
AvaI C'yCGr_G
Cuts at: 0 90 1185 1422
Size: 90 1095 237
Fragments arranged by size:
1095 237 90
BanII G_rGCy'C
Cuts at: 0 176 434 1422
Size: 176 258 988
Fragments arranged by size:
988 258 176
BbsI GAAGACnn'nnnn_
Cuts at: 0 1183 1422
Size: 1183 239
BbvCI CC'TCA_GC
Cuts at: 0 1362 1422
Size: 1362 60
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1355 1389 1422
Size: 1355 34 33
Fragments arranged by size:
1355 34 33
BlpI GC'TnA_GC
Cuts at: 0 314 1422
Size: 314 1108
BmrI ACTGGGnnnn_n'
Cuts at: 0 1303 1422
Size: 1303 119
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 851 967 1422
Size: 851 116 455
Fragments arranged by size:
851 455 116
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 250 420 562 746 892 895 1422
Size: 187 63 170 142 184 146 3 527
Fragments arranged by size:
527 187 184 170 146 142 63 3
Bpu10I CC'TnA_GC
Cuts at: 0 1362 1422
Size: 1362 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 924 1422
Size: 924 498
BsaI GGTCTCn'nnnn_
Cuts at: 0 226 1422
Size: 226 1196
BsaAI yAC'GTr
Cuts at: 0 1197 1422
Size: 1197 225
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1059 1089 1269 1299 1396 1422
Size: 1059 30 180 30 97 26
Fragments arranged by size:
1059 180 97 30 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 184 280 889 1401 1422
Size: 184 96 609 512 21
Fragments arranged by size:
609 512 184 96 21
BseYI C'CCAG_C
Cuts at: 0 1415 1422
Size: 1415 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1007 1219 1422
Size: 1007 212 203
Fragments arranged by size:
1007 212 203
BsiEI CG_ry'CG
Cuts at: 0 62 1422
Size: 62 1360
BsiHKAI G_wGCw'C
Cuts at: 0 176 434 1422
Size: 176 258 988
Fragments arranged by size:
988 258 176
BsmI GAATG_Cn'
Cuts at: 0 846 1422
Size: 846 576
BsmBI CGTCTCn'nnnn_
Cuts at: 0 579 720 1422
Size: 579 141 702
Fragments arranged by size:
702 579 141
Bsp1286I G_dGCh'C
Cuts at: 0 176 434 1422
Size: 176 258 988
Fragments arranged by size:
988 258 176
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 287 1422
Size: 287 1135
BstXI CCAn_nnnn'nTGG
Cuts at: 0 186 1422
Size: 186 1236
BtgI C'CryG_G
Cuts at: 0 746 1218 1240 1352 1422
Size: 746 472 22 112 70
Fragments arranged by size:
746 472 112 70 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 713 1422
Size: 713 709
BtsI GCAGTG_nn'
Cuts at: 0 256 1422
Size: 256 1166
DraI TTT'AAA
Cuts at: 0 794 1422
Size: 794 628
DrdI GACnn_nn'nnGTC
Cuts at: 0 712 1422
Size: 712 710
EaeI y'GGCC_r
Cuts at: 0 59 1422
Size: 59 1363
EagI C'GGCC_G
Cuts at: 0 59 1422
Size: 59 1363
EarI CTCTTCn'nnn_
Cuts at: 0 890 936 1422
Size: 890 46 486
Fragments arranged by size:
890 486 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 151 1313 1325 1422
Size: 19 132 1162 12 97
Fragments arranged by size:
1162 132 97 19 12
EcoICRI GAG'CTC
Cuts at: 0 174 432 1422
Size: 174 258 990
Fragments arranged by size:
990 258 174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 238 250 265 420 562 746 835
Size: 187 51 12 15 155 142 184 89
Cuts at: 835 892 895 1422
Size: 57 3 527
Fragments arranged by size:
527 187 184 155 142 89 57 51
15 12 3
EcoRI G'AATT_C
Cuts at: 0 328 415 1422
Size: 328 87 1007
Fragments arranged by size:
1007 328 87
EcoRV GAT'ATC
Cuts at: 0 741 1422
Size: 741 681
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 403 1422
Size: 403 1019
FspI TGC'GCA
Cuts at: 0 283 1422
Size: 283 1139
HaeII r_GCGC'y
Cuts at: 0 14 1422
Size: 14 1408
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 744 776 961 993 1422
Size: 744 32 185 32 429
Fragments arranged by size:
744 429 185 32 32
HincII GTy'rAC
Cuts at: 0 86 513 958 1422
Size: 86 427 445 464
Fragments arranged by size:
464 445 427 86
HindIII A'AGCT_T
Cuts at: 0 667 1422
Size: 667 755
NcoI C'CATG_G
Cuts at: 0 746 1218 1422
Size: 746 472 204
Fragments arranged by size:
746 472 204
NdeI CA'TA_TG
Cuts at: 0 1113 1422
Size: 1113 309
PmlI CAC'GTG
Cuts at: 0 1197 1422
Size: 1197 225
PshAI GACnn'nnGTC
Cuts at: 0 1309 1422
Size: 1309 113
PstI C_TGCA'G
Cuts at: 0 200 263 695 926 1422
Size: 200 63 432 231 496
Fragments arranged by size:
496 432 231 200 63
SacI G_AGCT'C
Cuts at: 0 176 434 1422
Size: 176 258 988
Fragments arranged by size:
988 258 176
SalI G'TCGA_C
Cuts at: 0 84 956 1422
Size: 84 872 466
Fragments arranged by size:
872 466 84
SbfI CC_TGCA'GG
Cuts at: 0 695 1422
Size: 695 727
SexAI A'CCwGG_T
Cuts at: 0 1101 1422
Size: 1101 321
SfcI C'TryA_G
Cuts at: 0 196 259 691 922 1422
Size: 196 63 432 231 500
Fragments arranged by size:
500 432 231 196 63
SmaI CCC'GGG
Cuts at: 0 1187 1422
Size: 1187 235
SmlI C'TyrA_G
Cuts at: 0 903 1422
Size: 903 519
SpeI A'CTAG_T
Cuts at: 0 636 1422
Size: 636 786
StuI AGG'CCT
Cuts at: 0 1092 1233 1422
Size: 1092 141 189
Fragments arranged by size:
1092 189 141
StyI C'CwwG_G
Cuts at: 0 746 1218 1422
Size: 746 472 204
Fragments arranged by size:
746 472 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1356 1422
Size: 1356 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1059 1091 1269 1301 1396 1422
Size: 1059 32 178 32 95 26
Fragments arranged by size:
1059 178 95 32 32 26
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 987 1422
Size: 987 435
XmaI C'CCGG_G
Cuts at: 0 1185 1422
Size: 1185 237
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApoI AvaI BanII BbsI
BbvCI BcgI BlpI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BstXI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
NcoI NdeI PmlI PshAI PstI SacI SalI SbfI
SexAI SfcI SmaI SmlI SpeI StuI StyI TaqII
TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BciVI BclI BfrBI
BglI BglII Bme1580I BmgBI BmtI BsaBI BsaHI BsaWI
BsiWI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoO109I FseI FspAI HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SanDI
SapI ScaI SfiI SfoI SgfI SgrAI SnaBI SphI
SrfI SspI SwaI TatI Tth111I XbaI XhoI XmnI
ZraI