Restriction map for
pFN21AE2183
(Linear) (Six-base) MAPSORT of: KIEE2183.seq Check: 3255 from: 1 to: 1791
KIEE2183.orf
With 182 enzymes: SgfI *
July 3, 2012 17:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 894 1791
Size: 894 897
Acc65I G'GTAC_C
Cuts at: 0 1260 1791
Size: 1260 531
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 1658 1791
Size: 277 1381 133
Fragments arranged by size:
1381 277 133
AflII C'TTAA_G
Cuts at: 0 928 1791
Size: 928 863
AhdI GACnn_n'nnGTC
Cuts at: 0 180 1791
Size: 180 1611
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 115 1791
Size: 115 1676
AlwNI CAG_nnn'CTG
Cuts at: 0 1053 1791
Size: 1053 738
ApoI r'AATT_y
Cuts at: 0 204 677 1131 1681 1791
Size: 204 473 454 550 110
Fragments arranged by size:
550 473 454 204 110
AseI AT'TA_AT
Cuts at: 0 959 1595 1791
Size: 959 636 196
Fragments arranged by size:
959 636 196
BanI G'GyrC_C
Cuts at: 0 1260 1791
Size: 1260 531
BanII G_rGCy'C
Cuts at: 0 1001 1092 1791
Size: 1001 91 699
Fragments arranged by size:
1001 699 91
BbsI GAAGACnn'nnnn_
Cuts at: 0 1646 1791
Size: 1646 145
BbvCI CC'TCA_GC
Cuts at: 0 974 1791
Size: 974 817
BfrBI ATG'CAT
Cuts at: 0 114 1791
Size: 114 1677
BglII A'GATC_T
Cuts at: 0 150 159 435 1143 1791
Size: 150 9 276 708 648
Fragments arranged by size:
708 648 276 150 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1076 1630 1791
Size: 1076 554 161
Fragments arranged by size:
1076 554 161
Bpu10I CC'TnA_GC
Cuts at: 0 974 1791
Size: 974 817
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 1791
Size: 781 1010
BsaWI w'CCGG_w
Cuts at: 0 6 1791
Size: 6 1785
BseYI C'CCAG_C
Cuts at: 0 1000 1689 1791
Size: 1000 689 102
Fragments arranged by size:
1000 689 102
BsiHKAI G_wGCw'C
Cuts at: 0 1092 1791
Size: 1092 699
Bsp1286I G_dGCh'C
Cuts at: 0 1001 1092 1791
Size: 1001 91 699
Fragments arranged by size:
1001 699 91
BspHI T'CATG_A
Cuts at: 0 53 1791
Size: 53 1738
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 894 1605 1614 1767 1791
Size: 894 711 9 153 24
Fragments arranged by size:
894 711 153 24 9
BssSI C'ACGA_G
Cuts at: 0 1675 1791
Size: 1675 116
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1620 1791
Size: 1620 171
BstYI r'GATC_y
Cuts at: 0 150 159 435 1143 1791
Size: 150 9 276 708 648
Fragments arranged by size:
708 648 276 150 9
DraI TTT'AAA
Cuts at: 0 1445 1791
Size: 1445 346
EcoICRI GAG'CTC
Cuts at: 0 1090 1791
Size: 1090 701
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 1076 1630 1658 1791
Size: 277 799 554 28 133
Fragments arranged by size:
799 554 277 133 28
EcoO109I rG'GnC_Cy
Cuts at: 0 1023 1633 1791
Size: 1023 610 158
Fragments arranged by size:
1023 610 158
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 425 1791
Size: 425 1366
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 379 411 1528 1560 1791
Size: 166 32 181 32 1117 32 231
Fragments arranged by size:
1117 231 181 166 32 32 32
HincII GTy'rAC
Cuts at: 0 37 238 1791
Size: 37 201 1553
Fragments arranged by size:
1553 201 37
KpnI G_GTAC'C
Cuts at: 0 1264 1791
Size: 1264 527
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1554 1791
Size: 1554 237
MslI CAynn'nnrTG
Cuts at: 0 97 1791
Size: 97 1694
NsiI A_TGCA'T
Cuts at: 0 116 1791
Size: 116 1675
PpuMI rG'GwC_Cy
Cuts at: 0 1633 1791
Size: 1633 158
PsiI TTA'TAA
Cuts at: 0 482 764 896 1106 1791
Size: 482 282 132 210 685
Fragments arranged by size:
685 482 282 210 132
PstI C_TGCA'G
Cuts at: 0 1254 1764 1791
Size: 1254 510 27
Fragments arranged by size:
1254 510 27
SacI G_AGCT'C
Cuts at: 0 1092 1791
Size: 1092 699
ScaI AGT'ACT
Cuts at: 0 1781 1791
Size: 1781 10
SfcI C'TryA_G
Cuts at: 0 537 735 1250 1584 1760 1791
Size: 537 198 515 334 176 31
Fragments arranged by size:
537 515 334 198 176 31
SmlI C'TyrA_G
Cuts at: 0 796 928 1791
Size: 796 132 863
Fragments arranged by size:
863 796 132
StuI AGG'CCT
Cuts at: 0 770 870 1791
Size: 770 100 921
Fragments arranged by size:
921 770 100
StyI C'CwwG_G
Cuts at: 0 1264 1791
Size: 1264 527
TatI w'GTAC_w
Cuts at: 0 1779 1791
Size: 1779 12
Enzymes that do cut:
AarI Acc65I AcuI AflII AhdI AlfI AlwNI ApoI
AseI BanI BanII BbsI BbvCI BfrBI BglII BpmI
Bpu10I BpuEI BsaWI BseYI BsiHKAI Bsp1286I BspHI BspMI
BssSI BstAPI BstYI DraI EcoICRI Eco57MI EcoO109I FalI
Hin4I HincII KpnI MmeI MslI NsiI PpuMI PsiI
PstI SacI ScaI SfcI SmlI StuI StyI TatI
Enzymes that do not cut:
AatII AccI AclI AfeI AflIII AgeI AleI AloI
ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI
BbeI BcgI BciVI BclI BglI BlpI Bme1580I BmgBI
BmrI BmtI BplI BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BsgI BsiEI BsiWI BsmI BsmBI BspEI BsrBI
BsrDI BsrFI BsrGI BssHII BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI
EaeI EagI EarI EciI EcoNI EcoRI EcoRV FseI
FspI FspAI HaeII HindIII HpaI KasI MfeI MluI
MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI