(Linear) (Six-base) MAPSORT of: KIEE2179.seq  Check: 435  from: 1  to: 843



KIEE2179.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:55  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     843 
   Size:        145     698
 
AflII C'TTAA_G

Cuts at:      0     748     843 
   Size:        748      95
 
AflIII A'CryG_T

Cuts at:      0     104     843 
   Size:        104     739
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     509     541     843 
   Size:        509      32     302

  Fragments arranged by size: 

                 509     302      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     227     843 
   Size:        227     616
 
ApoI r'AATT_y

Cuts at:      0     764     843 
   Size:        764      79
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     401     843 
   Size:        401     442
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     389     843 
   Size:        389     454
 
Bpu10I CC'TnA_GC

Cuts at:      0     490     843 
   Size:        490     353
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     525     843 
   Size:        525     318
 
BsaAI yAC'GTr

Cuts at:      0     105     843 
   Size:        105     738
 
BsaBI GATnn'nnATC

Cuts at:      0      58     843 
   Size:         58     785
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     130     160     843 
   Size:        130      30     683

  Fragments arranged by size: 

                 683     130      30
 
BseYI C'CCAG_C

Cuts at:      0      28     843 
   Size:         28     815
 
BsiHKAI G_wGCw'C

Cuts at:      0      74     791     843 
   Size:         74     717      52

  Fragments arranged by size: 

                 717      74      52
 
BsmI GAATG_Cn'

Cuts at:      0     480     843 
   Size:        480     363
 
Bsp1286I G_dGCh'C

Cuts at:      0      74     791     843 
   Size:         74     717      52

  Fragments arranged by size: 

                 717      74      52
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      52     843 
   Size:         52     791
 
BsrDI GCAATG_nn'

Cuts at:      0     158     843 
   Size:        158     685
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     223     258     843 
   Size:        223      35     585

  Fragments arranged by size: 

                 585     223      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     203     843 
   Size:        203     640
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     843 
   Size:        145     698
 
EcoO109I rG'GnC_Cy

Cuts at:      0      25     195     352     827     843 
   Size:         25     170     157     475      16

  Fragments arranged by size: 

                 475     170     157      25      16
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     130     162     843 
   Size:        130      32     681

  Fragments arranged by size: 

                 681     130      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      25     195     352     843 
   Size:         25     170     157     491

  Fragments arranged by size: 

                 491     170     157      25
 
PstI C_TGCA'G

Cuts at:      0      80     843 
   Size:         80     763
 
SanDI GG'GwC_CC

Cuts at:      0      25     195     843 
   Size:         25     170     648

  Fragments arranged by size: 

                 648     170      25
 
ScaI AGT'ACT

Cuts at:      0     145     843 
   Size:        145     698
 
SfcI C'TryA_G

Cuts at:      0      76     302     843 
   Size:         76     226     541

  Fragments arranged by size: 

                 541     226      76
 
SmlI C'TyrA_G

Cuts at:      0     504     748     843 
   Size:        504     244      95

  Fragments arranged by size: 

                 504     244      95
 
SspI AAT'ATT

Cuts at:      0     327     740     843 
   Size:        327     413     103

  Fragments arranged by size: 

                 413     327     103
 
TatI w'GTAC_w

Cuts at:      0     107     143     843 
   Size:        107      36     700

  Fragments arranged by size: 

                 700     107      36
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     630     662     843 
   Size:        630      32     181

  Fragments arranged by size: 

                 630     181      32
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AloI    AlwNI     ApoI     BbsI     BmrI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaXI    BseYI  BsiHKAI     BsmI
 Bsp1286I    BspMI    BsrDI    CspCI     EarI  Eco57MI EcoO109I    Hin4I
    PpuMI     PstI    SanDI     ScaI     SfcI     SmlI     SspI     TatI
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI     BsaI    BsaHI    BsaWI    BseRI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI