(Linear) (Six-base) MAPSORT of: KIEE2175.seq  Check: 8595  from: 1  to: 372



KIEE2175.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:55  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     225     372 
   Size:        225     147
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     161     203     372 
   Size:        161      42     169

  Fragments arranged by size: 

                 169     161      42
 
AfeI AGC'GCT

Cuts at:      0     298     372 
   Size:        298      74
 
AleI CACnn'nnGTG

Cuts at:      0      31     175     372 
   Size:         31     144     197

  Fragments arranged by size: 

                 197     144      31
 
AlwNI CAG_nnn'CTG

Cuts at:      0     339     372 
   Size:        339      33
 
ApoI r'AATT_y

Cuts at:      0      50     372 
   Size:         50     322
 
AvaI C'yCGr_G

Cuts at:      0      12     372 
   Size:         12     360
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     149     372 
   Size:        149     223
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     319     372 
   Size:        319      53
 
BseYI C'CCAG_C

Cuts at:      0     205     245     372 
   Size:        205      40     127

  Fragments arranged by size: 

                 205     127      40
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     225     372 
   Size:        225     147
 
BsrFI r'CCGG_y

Cuts at:      0      21     372 
   Size:         21     351
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     339     363     372 
   Size:        339      24       9

  Fragments arranged by size: 

                 339      24       9
 
BstEII G'GTnAC_C

Cuts at:      0      24     372 
   Size:         24     348
 
BtgI C'CryG_G

Cuts at:      0      32     257     372 
   Size:         32     225     115

  Fragments arranged by size: 

                 225     115      32
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     333     372 
   Size:        333      39
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     161     203     372 
   Size:        161      42     169

  Fragments arranged by size: 

                 169     161      42
 
EcoO109I rG'GnC_Cy

Cuts at:      0     117     190     372 
   Size:        117      73     182

  Fragments arranged by size: 

                 182     117      73
 
HaeII r_GCGC'y

Cuts at:      0     300     372 
   Size:        300      72
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      92     124     212     244     372 
   Size:         92      32      88      32     128

  Fragments arranged by size: 

                 128      92      88      32      32
 
MslI CAynn'nnrTG

Cuts at:      0      31     175     235     256     372 
   Size:         31     144      60      21     116

  Fragments arranged by size: 

                 144     116      60      31      21
 
NcoI C'CATG_G

Cuts at:      0     257     372 
   Size:        257     115
 
PpuMI rG'GwC_Cy

Cuts at:      0     117     372 
   Size:        117     255
 
PshAI GACnn'nnGTC

Cuts at:      0     259     372 
   Size:        259     113
 
PstI C_TGCA'G

Cuts at:      0     215     344     372 
   Size:        215     129      28

  Fragments arranged by size: 

                 215     129      28
 
SfcI C'TryA_G

Cuts at:      0     211     340     372 
   Size:        211     129      32

  Fragments arranged by size: 

                 211     129      32
 
SmaI CCC'GGG

Cuts at:      0      14     372 
   Size:         14     358
 
StyI C'CwwG_G

Cuts at:      0     257     372 
   Size:        257     115
 
XmaI C'CCGG_G

Cuts at:      0      12     372 
   Size:         12     360
 
XmnI GAAnn'nnTTC

Cuts at:      0     133     372 
   Size:        133     239
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AleI    AlwNI     ApoI     AvaI     BbsI
    BseRI    BseYI    BspMI    BsrFI   BstAPI   BstEII     BtgI     DrdI
  Eco57MI EcoO109I    HaeII    Hin4I     MslI     NcoI    PpuMI    PshAI
     PstI     SfcI     SmaI     StyI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     ZraI