Restriction map for
pFN21AE2166
(Linear) (Six-base) MAPSORT of: KIEE2166.seq Check: 1528 from: 1 to: 1059
KIEE2166.orf
With 182 enzymes: SgfI *
July 15, 2010 18:55 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 1059
Size: 236 823
AflIII A'CryG_T
Cuts at: 0 944 1059
Size: 944 115
AleI CACnn'nnGTG
Cuts at: 0 546 1059
Size: 546 513
ApaI G_GGCC'C
Cuts at: 0 751 1059
Size: 751 308
AvaI C'yCGr_G
Cuts at: 0 901 1059
Size: 901 158
BanI G'GyrC_C
Cuts at: 0 40 377 625 1059
Size: 40 337 248 434
Fragments arranged by size:
434 337 248 40
BanII G_rGCy'C
Cuts at: 0 137 503 751 1059
Size: 137 366 248 308
Fragments arranged by size:
366 308 248 137
BbeI G_GCGC'C
Cuts at: 0 44 1059
Size: 44 1015
BbsI GAAGACnn'nnnn_
Cuts at: 0 762 1059
Size: 762 297
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 957 991 1059
Size: 957 34 68
Fragments arranged by size:
957 68 34
Bme1580I G_kGCm'C
Cuts at: 0 628 751 970 996 1059
Size: 628 123 219 26 63
Fragments arranged by size:
628 219 123 63 26
BmgBI CAC'GTC
Cuts at: 0 986 1059
Size: 986 73
BmrI ACTGGGnnnn_n'
Cuts at: 0 624 1059
Size: 624 435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 265 516 1059
Size: 209 56 251 543
Fragments arranged by size:
543 251 209 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 1059
Size: 577 482
BsaAI yAC'GTr
Cuts at: 0 327 945 1059
Size: 327 618 114
Fragments arranged by size:
618 327 114
BsaHI Gr'CG_yC
Cuts at: 0 41 527 824 1059
Size: 41 486 297 235
Fragments arranged by size:
486 297 235 41
BsaWI w'CCGG_w
Cuts at: 0 257 1059
Size: 257 802
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 919 1059
Size: 919 140
BseYI C'CCAG_C
Cuts at: 0 494 621 1004 1059
Size: 494 127 383 55
Fragments arranged by size:
494 383 127 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 505 1059
Size: 45 460 554
Fragments arranged by size:
554 460 45
BsiEI CG_ry'CG
Cuts at: 0 302 482 1059
Size: 302 180 577
Fragments arranged by size:
577 302 180
BsiHKAI G_wGCw'C
Cuts at: 0 289 521 1059
Size: 289 232 538
Fragments arranged by size:
538 289 232
BsmBI CGTCTCn'nnnn_
Cuts at: 0 105 1059
Size: 105 954
Bsp1286I G_dGCh'C
Cuts at: 0 137 289 503 521 628 751 970 996
Size: 137 152 214 18 107 123 219 26
Cuts at: 996 1059
Size: 63
Fragments arranged by size:
219 214 152 137 123 107 63 26
18
BspHI T'CATG_A
Cuts at: 0 107 1059
Size: 107 952
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 170 443 515 608 1059
Size: 170 273 72 93 451
Fragments arranged by size:
451 273 170 93 72
BsrFI r'CCGG_y
Cuts at: 0 674 1059
Size: 674 385
BssSI C'ACGA_G
Cuts at: 0 679 1036 1059
Size: 679 357 23
Fragments arranged by size:
679 357 23
BstEII G'GTnAC_C
Cuts at: 0 739 1059
Size: 739 320
BstXI CCAn_nnnn'nTGG
Cuts at: 0 621 1059
Size: 621 438
BstYI r'GATC_y
Cuts at: 0 173 731 1059
Size: 173 558 328
Fragments arranged by size:
558 328 173
Bsu36I CC'TnA_GG
Cuts at: 0 842 1059
Size: 842 217
BtgI C'CryG_G
Cuts at: 0 213 819 913 1059
Size: 213 606 94 146
Fragments arranged by size:
606 213 146 94
BtsI GCAGTG_nn'
Cuts at: 0 994 1059
Size: 994 65
DraIII CAC_nnn'GTG
Cuts at: 0 126 1059
Size: 126 933
EaeI y'GGCC_r
Cuts at: 0 299 464 479 800 1059
Size: 299 165 15 321 259
Fragments arranged by size:
321 299 259 165 15
EagI C'GGCC_G
Cuts at: 0 299 479 1059
Size: 299 180 580
Fragments arranged by size:
580 299 180
EarI CTCTTCn'nnn_
Cuts at: 0 952 1059
Size: 952 107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 236 265 516 1059
Size: 209 27 29 251 543
Fragments arranged by size:
543 251 209 29 27
EcoO109I rG'GnC_Cy
Cuts at: 0 747 1059
Size: 747 312
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 900 1059
Size: 900 159
HaeII r_GCGC'y
Cuts at: 0 44 495 675 694 1059
Size: 44 451 180 19 365
Fragments arranged by size:
451 365 180 44 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 723 755 970 1002 1059
Size: 723 32 215 32 57
Fragments arranged by size:
723 215 57 32 32
KasI G'GCGC_C
Cuts at: 0 40 1059
Size: 40 1019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 1059
Size: 548 511
MslI CAynn'nnrTG
Cuts at: 0 546 1048 1059
Size: 546 502 11
Fragments arranged by size:
546 502 11
MspA1I CmG'CkG
Cuts at: 0 54 429 756 821 915 1008 1059
Size: 54 375 327 65 94 93 51
Fragments arranged by size:
375 327 94 93 65 54 51
NaeI GCC'GGC
Cuts at: 0 676 1059
Size: 676 383
NarI GG'CG_CC
Cuts at: 0 41 1059
Size: 41 1018
NgoMIV G'CCGG_C
Cuts at: 0 674 1059
Size: 674 385
NspI r_CATG'y
Cuts at: 0 702 1015 1059
Size: 702 313 44
Fragments arranged by size:
702 313 44
PflMI CCAn_nnn'nTGG
Cuts at: 0 330 863 1059
Size: 330 533 196
Fragments arranged by size:
533 330 196
PfoI T'CCnGG_A
Cuts at: 0 242 449 532 1059
Size: 242 207 83 527
Fragments arranged by size:
527 242 207 83
PmlI CAC'GTG
Cuts at: 0 327 945 1059
Size: 327 618 114
Fragments arranged by size:
618 327 114
PspOMI G'GGCC_C
Cuts at: 0 747 1059
Size: 747 312
PvuII CAG'CTG
Cuts at: 0 756 1008 1059
Size: 756 252 51
Fragments arranged by size:
756 252 51
SacII CC_GC'GG
Cuts at: 0 822 916 1059
Size: 822 94 143
Fragments arranged by size:
822 143 94
SapI GCTCTTCn'nnn_
Cuts at: 0 952 1059
Size: 952 107
ScaI AGT'ACT
Cuts at: 0 883 1059
Size: 883 176
SfoI GGC'GCC
Cuts at: 0 42 1059
Size: 42 1017
SmlI C'TyrA_G
Cuts at: 0 592 1059
Size: 592 467
SphI G_CATG'C
Cuts at: 0 702 1015 1059
Size: 702 313 44
Fragments arranged by size:
702 313 44
StuI AGG'CCT
Cuts at: 0 847 1059
Size: 847 212
StyI C'CwwG_G
Cuts at: 0 46 137 1059
Size: 46 91 922
Fragments arranged by size:
922 91 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 643 1059
Size: 643 416
TatI w'GTAC_w
Cuts at: 0 881 1052 1059
Size: 881 171 7
Fragments arranged by size:
881 171 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 304 336 1059
Size: 304 32 723
Fragments arranged by size:
723 304 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 795 996 1059
Size: 795 201 63
Fragments arranged by size:
795 201 63
Enzymes that do cut:
AcuI AflIII AleI ApaI AvaI BanI BanII BbeI
BbsI BcgI Bme1580I BmgBI BmrI BpmI BpuEI BsaAI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrFI BssSI BstEII BstXI BstYI
Bsu36I BtgI BtsI DraIII EaeI EagI EarI Eco57MI
EcoO109I FalI HaeII Hin4I KasI MmeI MslI MspA1I
NaeI NarI NgoMIV NspI PflMI PfoI PmlI PspOMI
PvuII SacII SapI ScaI SfoI SmlI SphI StuI
StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI
BglI BglII BlpI BmtI BplI Bpu10I BsaI BsaBI
BsaXI BsiWI BsmI BspEI BsrBI BsrDI BsrGI BssHII
BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MscI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SalI SanDI SbfI SexAI SfcI
SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI Tth111I XbaI XhoI XmaI XmnI ZraI