Restriction map for
pFN21AE2163
(Linear) (Six-base) MAPSORT of: KIEE2163.seq Check: 2404 from: 1 to: 2646
KIEE2163.orf
With 182 enzymes: SgfI *
July 15, 2010 18:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1335 2646
Size: 1335 1311
Acc65I G'GTAC_C
Cuts at: 0 1096 2138 2646
Size: 1096 1042 508
Fragments arranged by size:
1096 1042 508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 1411 2563 2646
Size: 604 807 1152 83
Fragments arranged by size:
1152 807 604 83
AfeI AGC'GCT
Cuts at: 0 2287 2646
Size: 2287 359
AflIII A'CryG_T
Cuts at: 0 38 1166 1798 2646
Size: 38 1128 632 848
Fragments arranged by size:
1128 848 632 38
AhdI GACnn_n'nnGTC
Cuts at: 0 1782 2646
Size: 1782 864
AlwNI CAG_nnn'CTG
Cuts at: 0 807 1680 2319 2353 2646
Size: 807 873 639 34 293
Fragments arranged by size:
873 807 639 293 34
ApaLI G'TGCA_C
Cuts at: 0 1315 2646
Size: 1315 1331
ApoI r'AATT_y
Cuts at: 0 1081 2646
Size: 1081 1565
AvaI C'yCGr_G
Cuts at: 0 154 1299 2512 2531 2646
Size: 154 1145 1213 19 115
Fragments arranged by size:
1213 1145 154 115 19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1080 1113 2646
Size: 1080 33 1533
Fragments arranged by size:
1533 1080 33
BanI G'GyrC_C
Cuts at: 0 1096 1287 1846 2055 2138 2450 2646
Size: 1096 191 559 209 83 312 196
Fragments arranged by size:
1096 559 312 209 196 191 83
BanII G_rGCy'C
Cuts at: 0 140 1541 2157 2576 2646
Size: 140 1401 616 419 70
Fragments arranged by size:
1401 616 419 140 70
BbeI G_GCGC'C
Cuts at: 0 1291 2059 2646
Size: 1291 768 587
Fragments arranged by size:
1291 768 587
BbsI GAAGACnn'nnnn_
Cuts at: 0 870 1801 2646
Size: 870 931 845
Fragments arranged by size:
931 870 845
BbvCI CC'TCA_GC
Cuts at: 0 1106 2646
Size: 1106 1540
BglII A'GATC_T
Cuts at: 0 1715 2646
Size: 1715 931
Bme1580I G_kGCm'C
Cuts at: 0 982 1319 2238 2646
Size: 982 337 919 408
Fragments arranged by size:
982 919 408 337
BmgBI CAC'GTC
Cuts at: 0 1002 1799 2646
Size: 1002 797 847
Fragments arranged by size:
1002 847 797
BmrI ACTGGGnnnn_n'
Cuts at: 0 185 295 2161 2646
Size: 185 110 1866 485
Fragments arranged by size:
1866 485 185 110
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 331 1488 2224 2617 2646
Size: 154 177 1157 736 393 29
Fragments arranged by size:
1157 736 393 177 154 29
Bpu10I CC'TnA_GC
Cuts at: 0 1106 1888 2646
Size: 1106 782 758
Fragments arranged by size:
1106 782 758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 409 529 2646
Size: 193 216 120 2117
Fragments arranged by size:
2117 216 193 120
BsaI GGTCTCn'nnnn_
Cuts at: 0 1906 2646
Size: 1906 740
BsaAI yAC'GTr
Cuts at: 0 39 624 1320 2646
Size: 39 585 696 1326
Fragments arranged by size:
1326 696 585 39
BsaHI Gr'CG_yC
Cuts at: 0 443 764 1288 2056 2646
Size: 443 321 524 768 590
Fragments arranged by size:
768 590 524 443 321
BsaWI w'CCGG_w
Cuts at: 0 20 2646
Size: 20 2626
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 15 45 1138 1168 1783 1813 1915 1945
Size: 15 30 1093 30 615 30 102 30
Cuts at: 1945 1990 2020 2646
Size: 45 30 626
Fragments arranged by size:
1093 626 615 102 45 30 30 30
30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1390 1546 1741 1810 1936 2646
Size: 1390 156 195 69 126 710
Fragments arranged by size:
1390 710 195 156 126 69
BseYI C'CCAG_C
Cuts at: 0 1114 1903 2646
Size: 1114 789 743
Fragments arranged by size:
1114 789 743
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 550 764 769 799 850 1342 1633
Size: 238 312 214 5 30 51 492 291
Cuts at: 1633 1711 2395 2646
Size: 78 684 251
Fragments arranged by size:
684 492 312 291 251 238 214 78
51 30 5
BsiHKAI G_wGCw'C
Cuts at: 0 659 1319 1541 1556 2576 2646
Size: 659 660 222 15 1020 70
Fragments arranged by size:
1020 660 659 222 70 15
BsmI GAATG_Cn'
Cuts at: 0 1259 2646
Size: 1259 1387
Bsp1286I G_dGCh'C
Cuts at: 0 140 659 982 1319 1541 1556 2157 2238
Size: 140 519 323 337 222 15 601 81
Cuts at: 2238 2576 2646
Size: 338 70
Fragments arranged by size:
601 519 338 337 323 222 140 81
70 15
BspEI T'CCGG_A
Cuts at: 0 20 2646
Size: 20 2626
BspHI T'CATG_A
Cuts at: 0 1577 2646
Size: 1577 1069
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1335 1462 2361 2646
Size: 1335 127 899 285
Fragments arranged by size:
1335 899 285 127
BsrBI CCG'CTC
Cuts at: 0 1740 2646
Size: 1740 906
BsrFI r'CCGG_y
Cuts at: 0 766 2206 2646
Size: 766 1440 440
Fragments arranged by size:
1440 766 440
BsrGI T'GTAC_A
Cuts at: 0 1619 2646
Size: 1619 1027
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1638 1680 2646
Size: 1638 42 966
Fragments arranged by size:
1638 966 42
BstEII G'GTnAC_C
Cuts at: 0 2391 2646
Size: 2391 255
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1272 1512 2364 2484 2646
Size: 1272 240 852 120 162
Fragments arranged by size:
1272 852 240 162 120
BstYI r'GATC_y
Cuts at: 0 1715 1925 2646
Size: 1715 210 721
Fragments arranged by size:
1715 721 210
BtgI C'CryG_G
Cuts at: 0 387 890 1410 1444 2117 2646
Size: 387 503 520 34 673 529
Fragments arranged by size:
673 529 520 503 387 34
BtsI GCAGTG_nn'
Cuts at: 0 781 1289 1678 1850 2646
Size: 781 508 389 172 796
Fragments arranged by size:
796 781 508 389 172
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 369 404 2646
Size: 369 35 2242
Fragments arranged by size:
2242 369 35
DraIII CAC_nnn'GTG
Cuts at: 0 1638 2646
Size: 1638 1008
DrdI GACnn_nn'nnGTC
Cuts at: 0 732 1587 2646
Size: 732 855 1059
Fragments arranged by size:
1059 855 732
EaeI y'GGCC_r
Cuts at: 0 302 390 719 1214 1941 2646
Size: 302 88 329 495 727 705
Fragments arranged by size:
727 705 495 329 302 88
EarI CTCTTCn'nnn_
Cuts at: 0 1468 1546 2646
Size: 1468 78 1100
Fragments arranged by size:
1468 1100 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 160 2646
Size: 160 2486
EcoICRI GAG'CTC
Cuts at: 0 1539 2574 2646
Size: 1539 1035 72
Fragments arranged by size:
1539 1035 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 331 604 1411 1488 2224 2563 2617
Size: 154 177 273 807 77 736 339 54
Cuts at: 2617 2646
Size: 29
Fragments arranged by size:
807 736 339 273 177 154 77 54
29
EcoO109I rG'GnC_Cy
Cuts at: 0 171 804 1008 1734 1749 2094 2628 2646
Size: 171 633 204 726 15 345 534 18
Fragments arranged by size:
726 633 534 345 204 171 18 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 894 2202 2646
Size: 894 1308 444
Fragments arranged by size:
1308 894 444
FspI TGC'GCA
Cuts at: 0 539 2646
Size: 539 2107
HaeII r_GCGC'y
Cuts at: 0 915 1291 2033 2059 2289 2646
Size: 915 376 742 26 230 357
Fragments arranged by size:
915 742 376 357 230 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 15 47 1768 1800 2206 2238 2646
Size: 15 32 1721 32 406 32 408
Fragments arranged by size:
1721 408 406 32 32 32 15
HincII GTy'rAC
Cuts at: 0 435 2646
Size: 435 2211
HindIII A'AGCT_T
Cuts at: 0 871 2646
Size: 871 1775
KasI G'GCGC_C
Cuts at: 0 1287 2055 2646
Size: 1287 768 591
Fragments arranged by size:
1287 768 591
KpnI G_GTAC'C
Cuts at: 0 1100 2142 2646
Size: 1100 1042 504
Fragments arranged by size:
1100 1042 504
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1616 2021 2033 2646
Size: 1616 405 12 613
Fragments arranged by size:
1616 613 405 12
MscI TGG'CCA
Cuts at: 0 304 721 1943 2646
Size: 304 417 1222 703
Fragments arranged by size:
1222 703 417 304
MslI CAynn'nnrTG
Cuts at: 0 943 964 985 991 1270 1360 1576 2646
Size: 943 21 21 6 279 90 216 1070
Fragments arranged by size:
1070 943 279 216 90 21 21 6
MspA1I CmG'CkG
Cuts at: 0 615 1326 2119 2249 2646
Size: 615 711 793 130 397
Fragments arranged by size:
793 711 615 397 130
NaeI GCC'GGC
Cuts at: 0 768 2208 2646
Size: 768 1440 438
Fragments arranged by size:
1440 768 438
NarI GG'CG_CC
Cuts at: 0 1288 2056 2646
Size: 1288 768 590
Fragments arranged by size:
1288 768 590
NgoMIV G'CCGG_C
Cuts at: 0 766 2206 2646
Size: 766 1440 440
Fragments arranged by size:
1440 766 440
NspI r_CATG'y
Cuts at: 0 17 366 778 1170 1182 1878 2646
Size: 17 349 412 392 12 696 768
Fragments arranged by size:
768 696 412 392 349 17 12
PciI A'CATG_T
Cuts at: 0 1166 2646
Size: 1166 1480
PflMI CCAn_nnn'nTGG
Cuts at: 0 297 807 2319 2646
Size: 297 510 1512 327
Fragments arranged by size:
1512 510 327 297
PfoI T'CCnGG_A
Cuts at: 0 308 800 1654 2646
Size: 308 492 854 992
Fragments arranged by size:
992 854 492 308
PmlI CAC'GTG
Cuts at: 0 39 1320 2646
Size: 39 1281 1326
Fragments arranged by size:
1326 1281 39
PpuMI rG'GwC_Cy
Cuts at: 0 171 804 1734 1749 2094 2628 2646
Size: 171 633 930 15 345 534 18
Fragments arranged by size:
930 633 534 345 171 18 15
PshAI GACnn'nnGTC
Cuts at: 0 1766 2646
Size: 1766 880
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1963 1995 2646
Size: 1963 32 651
Fragments arranged by size:
1963 651 32
PstI C_TGCA'G
Cuts at: 0 595 1676 2646
Size: 595 1081 970
Fragments arranged by size:
1081 970 595
PvuII CAG'CTG
Cuts at: 0 1326 2646
Size: 1326 1320
RsrII CG'GwC_CG
Cuts at: 0 2121 2646
Size: 2121 525
SacI G_AGCT'C
Cuts at: 0 1541 2576 2646
Size: 1541 1035 70
Fragments arranged by size:
1541 1035 70
SacII CC_GC'GG
Cuts at: 0 2120 2646
Size: 2120 526
SanDI GG'GwC_CC
Cuts at: 0 171 2646
Size: 171 2475
SapI GCTCTTCn'nnn_
Cuts at: 0 1546 2646
Size: 1546 1100
ScaI AGT'ACT
Cuts at: 0 472 1708 2646
Size: 472 1236 938
Fragments arranged by size:
1236 938 472
SexAI A'CCwGG_T
Cuts at: 0 1634 2646
Size: 1634 1012
SfcI C'TryA_G
Cuts at: 0 591 1672 2646
Size: 591 1081 974
Fragments arranged by size:
1081 974 591
SfoI GGC'GCC
Cuts at: 0 1289 2057 2646
Size: 1289 768 589
Fragments arranged by size:
1289 768 589
SmlI C'TyrA_G
Cuts at: 0 208 424 544 2646
Size: 208 216 120 2102
Fragments arranged by size:
2102 216 208 120
SphI G_CATG'C
Cuts at: 0 17 778 1878 2646
Size: 17 761 1100 768
Fragments arranged by size:
1100 768 761 17
StuI AGG'CCT
Cuts at: 0 131 2646
Size: 131 2515
StyI C'CwwG_G
Cuts at: 0 1787 2090 2385 2420 2646
Size: 1787 303 295 35 226
Fragments arranged by size:
1787 303 295 226 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2290 2646
Size: 2290 356
TatI w'GTAC_w
Cuts at: 0 470 1619 1706 2180 2646
Size: 470 1149 87 474 466
Fragments arranged by size:
1149 474 470 466 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1138 1170 1189 1221 1783 1815 2646
Size: 1138 32 19 32 562 32 831
Fragments arranged by size:
1138 831 562 32 32 32 19
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 750 1936 2316 2646
Size: 750 1186 380 330
Fragments arranged by size:
1186 750 380 330
XmnI GAAnn'nnTTC
Cuts at: 0 1084 1147 1708 2646
Size: 1084 63 561 938
Fragments arranged by size:
1084 938 561 63
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AhdI AlwNI ApaLI
ApoI AvaI BaeI BanI BanII BbeI BbsI BbvCI
BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI
BstAPI BstEII BstXI BstYI BtgI BtsI CspCI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PfoI PmlI PpuMI PshAI PsrI PstI PvuII
RsrII SacI SacII SanDI SapI ScaI SexAI SfcI
SfoI SmlI SphI StuI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AleI AlfI AloI
ApaI AscI AseI AsiSI AvrII BamHI BcgI BciVI
BclI BfrBI BglI BlpI BmtI BplI BsaBI BsiEI
BsiWI BsmBI BsrDI BssHII BssSI BstBI BstZ17I Bsu36I
BtgZI ClaI DraI EagI EcoNI EcoRI EcoRV FseI
FspAI HpaI MfeI MluI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PmeI PpiI PsiI PspOMI
PspXI PvuI SalI SbfI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI
XmaI ZraI