(Linear) (Six-base) MAPSORT of: KIEE2160.seq  Check: 1518  from: 1  to: 675



KIEE2160.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:55  ..

AccI GT'mk_AC

Cuts at:      0     653     675 
   Size:        653      22
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     412     675 
   Size:        412     263
 
AlwNI CAG_nnn'CTG

Cuts at:      0      67     675 
   Size:         67     608
 
ApaLI G'TGCA_C

Cuts at:      0     318     675 
   Size:        318     357
 
BanII G_rGCy'C

Cuts at:      0     540     675 
   Size:        540     135
 
Bme1580I G_kGCm'C

Cuts at:      0      61     322     675 
   Size:         61     261     353

  Fragments arranged by size: 

                 353     261      61
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     508     675 
   Size:        508     167
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     642     675 
   Size:        642      33
 
BsaBI GATnn'nnATC

Cuts at:      0     292     675 
   Size:        292     383
 
BsaWI w'CCGG_w

Cuts at:      0     222     631     675 
   Size:        222     409      44

  Fragments arranged by size: 

                 409     222      44
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     548     675 
   Size:        548     127
 
BseYI C'CCAG_C

Cuts at:      0      25     413     675 
   Size:         25     388     262

  Fragments arranged by size: 

                 388     262      25
 
BsiHKAI G_wGCw'C

Cuts at:      0     322     401     540     675 
   Size:        322      79     139     135

  Fragments arranged by size: 

                 322     139     135      79
 
Bsp1286I G_dGCh'C

Cuts at:      0      61     322     401     540     675 
   Size:         61     261      79     139     135

  Fragments arranged by size: 

                 261     139     135      79      61
 
BspEI T'CCGG_A

Cuts at:      0     222     675 
   Size:        222     453
 
BspHI T'CATG_A

Cuts at:      0     284     675 
   Size:        284     391
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      10     308     317     675 
   Size:         10     298       9     358

  Fragments arranged by size: 

                 358     298      10       9
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     516     675 
   Size:        516     159
 
BtgI C'CryG_G

Cuts at:      0     509     675 
   Size:        509     166
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     589     675 
   Size:        589      86
 
BtsI GCAGTG_nn'

Cuts at:      0     193     675 
   Size:        193     482
 
DraIII CAC_nnn'GTG

Cuts at:      0     317     675 
   Size:        317     358
 
EaeI y'GGCC_r

Cuts at:      0     290     675 
   Size:        290     385
 
EcoICRI GAG'CTC

Cuts at:      0     538     675 
   Size:        538     137
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     642     675 
   Size:        642      33
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      27     510     675 
   Size:         27     483     165

  Fragments arranged by size: 

                 483     165      27
 
MscI TGG'CCA

Cuts at:      0     292     675 
   Size:        292     383
 
MslI CAynn'nnrTG

Cuts at:      0      76     181     388     394     406     675 
   Size:         76     105     207       6      12     269

  Fragments arranged by size: 

                 269     207     105      76      12       6
 
SacI G_AGCT'C

Cuts at:      0     540     675 
   Size:        540     135
 
SexAI A'CCwGG_T

Cuts at:      0     313     675 
   Size:        313     362
 
SfcI C'TryA_G

Cuts at:      0     229     582     675 
   Size:        229     353      93

  Fragments arranged by size: 

                 353     229      93
 
StyI C'CwwG_G

Cuts at:      0     297     675 
   Size:        297     378
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     133     165     675 
   Size:        133      32     510

  Fragments arranged by size: 

                 510     133      32
 

 Enzymes that do cut:

     AccI     AlfI    AlwNI    ApaLI    BanII Bme1580I     BplI     BpmI
    BsaBI    BsaWI    BseRI    BseYI  BsiHKAI Bsp1286I    BspEI    BspHI
   BstAPI    BstXI     BtgI    BtgZI     BtsI   DraIII     EaeI  EcoICRI
  Eco57MI     MmeI     MscI     MslI     SacI    SexAI     SfcI     StyI
     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I     ClaI    CspCI     DraI     DrdI     EagI     EarI
     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI