(Linear) (Six-base) MAPSORT of: KIEE2158.seq  Check: 8374  from: 1  to: 621



KIEE2158.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:55  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     424     621 
   Size:        424     197
 
AlwNI CAG_nnn'CTG

Cuts at:      0      18     207     621 
   Size:         18     189     414

  Fragments arranged by size: 

                 414     189      18
 
ApaI G_GGCC'C

Cuts at:      0     150     176     621 
   Size:        150      26     445

  Fragments arranged by size: 

                 445     150      26
 
ApaLI G'TGCA_C

Cuts at:      0     497     621 
   Size:        497     124
 
ApoI r'AATT_y

Cuts at:      0       3     160     607     621 
   Size:          3     157     447      14

  Fragments arranged by size: 

                 447     157      14       3
 
BanII G_rGCy'C

Cuts at:      0     150     176     621 
   Size:        150      26     445

  Fragments arranged by size: 

                 445     150      26
 
BglI GCCn_nnn'nGGC

Cuts at:      0     223     621 
   Size:        223     398
 
Bme1580I G_kGCm'C

Cuts at:      0     150     176     270     501     621 
   Size:        150      26      94     231     120

  Fragments arranged by size: 

                 231     150     120      94      26
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     455     621 
   Size:        455     166
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     361     621 
   Size:        150     211     260

  Fragments arranged by size: 

                 260     211     150
 
Bpu10I CC'TnA_GC

Cuts at:      0     248     621 
   Size:        248     373
 
BsaWI w'CCGG_w

Cuts at:      0     521     621 
   Size:        521     100
 
BsiHKAI G_wGCw'C

Cuts at:      0     101     501     621 
   Size:        101     400     120

  Fragments arranged by size: 

                 400     120     101
 
BsmI GAATG_Cn'

Cuts at:      0     529     621 
   Size:        529      92
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     306     621 
   Size:        306     315
 
Bsp1286I G_dGCh'C

Cuts at:      0     101     150     176     270     501     621 
   Size:        101      49      26      94     231     120

  Fragments arranged by size: 

                 231     120     101      94      49      26
 
BspEI T'CCGG_A

Cuts at:      0     521     621 
   Size:        521     100
 
BssSI C'ACGA_G

Cuts at:      0     261     621 
   Size:        261     360
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      10      45     621 
   Size:         10      35     576

  Fragments arranged by size: 

                 576      35      10
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     405     621 
   Size:        405     216
 
EaeI y'GGCC_r

Cuts at:      0     422     621 
   Size:        422     199
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     361     424     621 
   Size:        150     211      63     197

  Fragments arranged by size: 

                 211     197     150      63
 
EcoO109I rG'GnC_Cy

Cuts at:      0     146     173     621 
   Size:        146      27     448

  Fragments arranged by size: 

                 448     146      27
 
EcoRI G'AATT_C

Cuts at:      0     160     621 
   Size:        160     461
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      63      95     621 
   Size:         63      32     526

  Fragments arranged by size: 

                 526      63      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     521     621 
   Size:        521     100
 
MscI TGG'CCA

Cuts at:      0     424     621 
   Size:        424     197
 
PasI CC'CwG_GG

Cuts at:      0     150     621 
   Size:        150     471
 
PspOMI G'GGCC_C

Cuts at:      0     146     172     621 
   Size:        146      26     449

  Fragments arranged by size: 

                 449     146      26
 
PstI C_TGCA'G

Cuts at:      0     281     296     621 
   Size:        281      15     325

  Fragments arranged by size: 

                 325     281      15
 
SfcI C'TryA_G

Cuts at:      0     277     292     621 
   Size:        277      15     329

  Fragments arranged by size: 

                 329     277      15
 
StyI C'CwwG_G

Cuts at:      0     570     621 
   Size:        570      51
 
XmnI GAAnn'nnTTC

Cuts at:      0     409     621 
   Size:        409     212
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI    ApaLI     ApoI    BanII     BglI Bme1580I
     BmrI     BpmI   Bpu10I    BsaWI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BssSI    CspCI     DrdI     EaeI  Eco57MI EcoO109I    EcoRI
    Hin4I     MmeI     MscI     PasI   PspOMI     PstI     SfcI     StyI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI
    BseYI     BsgI    BsiEI    BsiWI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI