Restriction map for
pFN21AE2153
(Linear) (Six-base) MAPSORT of: KIEE2153.seq Check: 5365 from: 1 to: 1839
KIEE2153.orf
With 182 enzymes: SgfI *
July 15, 2010 18:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 291 1053 1839
Size: 291 762 786
Fragments arranged by size:
786 762 291
AccI GT'mk_AC
Cuts at: 0 1709 1839
Size: 1709 130
Acc65I G'GTAC_C
Cuts at: 0 755 1670 1798 1839
Size: 755 915 128 41
Fragments arranged by size:
915 755 128 41
AflIII A'CryG_T
Cuts at: 0 1296 1839
Size: 1296 543
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 541 570 1839
Size: 541 29 1269
Fragments arranged by size:
1269 541 29
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 238 270 1839
Size: 238 32 1569
Fragments arranged by size:
1569 238 32
AlwNI CAG_nnn'CTG
Cuts at: 0 220 543 900 1643 1699 1839
Size: 220 323 357 743 56 140
Fragments arranged by size:
743 357 323 220 140 56
ApaI G_GGCC'C
Cuts at: 0 81 107 169 485 852 1064 1114 1571
Size: 81 26 62 316 367 212 50 457
Cuts at: 1571 1607 1839
Size: 36 232
Fragments arranged by size:
457 367 316 232 212 81 62 50
36 26
AvaI C'yCGr_G
Cuts at: 0 80 97 205 278 485 619 1606 1839
Size: 80 17 108 73 207 134 987 233
Fragments arranged by size:
987 233 207 134 108 80 73 17
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1751 1784 1839
Size: 1751 33 55
Fragments arranged by size:
1751 55 33
BamHI G'GATC_C
Cuts at: 0 831 1839
Size: 831 1008
BanI G'GyrC_C
Cuts at: 0 4 624 755 775 796 1041 1053 1204
Size: 4 620 131 20 21 245 12 151
Cuts at: 1204 1670 1783 1798 1839
Size: 466 113 15 41
Fragments arranged by size:
620 466 245 151 131 113 41 21
20 15 12 4
BanII G_rGCy'C
Cuts at: 0 81 107 169 485 696 852 962 1064
Size: 81 26 62 316 211 156 110 102
Cuts at: 1064 1114 1466 1571 1607 1839
Size: 50 352 105 36 232
Fragments arranged by size:
352 316 232 211 156 110 105 102
81 62 50 36 26
BbeI G_GCGC'C
Cuts at: 0 8 1839
Size: 8 1831
BbsI GAAGACnn'nnnn_
Cuts at: 0 261 1839
Size: 261 1578
BciVI GTATCCnnnnn_n'
Cuts at: 0 240 1839
Size: 240 1599
BfrBI ATG'CAT
Cuts at: 0 1722 1839
Size: 1722 117
BglI GCCn_nnn'nGGC
Cuts at: 0 685 877 1839
Size: 685 192 962
Fragments arranged by size:
962 685 192
BlpI GC'TnA_GC
Cuts at: 0 704 1465 1839
Size: 704 761 374
Fragments arranged by size:
761 704 374
Bme1580I G_kGCm'C
Cuts at: 0 81 107 169 485 620 780 852 993
Size: 81 26 62 316 135 160 72 141
Cuts at: 993 1056 1064 1114 1207 1275 1571 1607 1635
Size: 63 8 50 93 68 296 36 28
Cuts at: 1635 1689 1839
Size: 54 150
Fragments arranged by size:
316 296 160 150 141 135 93 81
72 68 63 62 54 50 36 28
26 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 235 246 559 1839
Size: 235 11 313 1280
Fragments arranged by size:
1280 313 235 11
BmtI G_CTAG'C
Cuts at: 0 1342 1839
Size: 1342 497
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1389 1485 1839
Size: 1389 96 354
Fragments arranged by size:
1389 354 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 710 1342 1556 1839
Size: 442 268 632 214 283
Fragments arranged by size:
632 442 283 268 214
Bpu10I CC'TnA_GC
Cuts at: 0 1692 1839
Size: 1692 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 1839
Size: 319 1520
BsaAI yAC'GTr
Cuts at: 0 885 1839
Size: 885 954
BsaHI Gr'CG_yC
Cuts at: 0 5 1839
Size: 5 1834
BsaWI w'CCGG_w
Cuts at: 0 665 1839
Size: 665 1174
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 373 403 1337 1367 1532 1562 1839
Size: 373 30 934 30 165 30 277
Fragments arranged by size:
934 373 277 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1553 1839
Size: 1553 286
BseYI C'CCAG_C
Cuts at: 0 74 173 845 999 1079 1481 1521 1636
Size: 74 99 672 154 80 402 40 115
Cuts at: 1636 1839
Size: 203
Fragments arranged by size:
672 402 203 154 115 99 80 74
40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 319 325 792 913 1669 1839
Size: 206 113 6 467 121 756 170
Fragments arranged by size:
756 467 206 170 121 113 6
BsiEI CG_ry'CG
Cuts at: 0 753 1068 1839
Size: 753 315 771
Fragments arranged by size:
771 753 315
BsiHKAI G_wGCw'C
Cuts at: 0 335 696 1466 1839
Size: 335 361 770 373
Fragments arranged by size:
770 373 361 335
Bsp1286I G_dGCh'C
Cuts at: 0 81 107 169 335 485 620 696 780
Size: 81 26 62 166 150 135 76 84
Cuts at: 780 852 962 993 1056 1064 1114 1207 1275
Size: 72 110 31 63 8 50 93 68
Cuts at: 1275 1466 1571 1607 1635 1689 1839
Size: 191 105 36 28 54 150
Fragments arranged by size:
191 166 150 150 135 110 105 93
84 81 76 72 68 63 62 54
50 36 31 28 26 8
BspHI T'CATG_A
Cuts at: 0 1817 1839
Size: 1817 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 291 1053 1839
Size: 291 762 786
Fragments arranged by size:
786 762 291
BsrBI CCG'CTC
Cuts at: 0 1691 1839
Size: 1691 148
BsrDI GCAATG_nn'
Cuts at: 0 153 984 1133 1839
Size: 153 831 149 706
Fragments arranged by size:
831 706 153 149
BsrFI r'CCGG_y
Cuts at: 0 154 364 1166 1839
Size: 154 210 802 673
Fragments arranged by size:
802 673 210 154
BsrGI T'GTAC_A
Cuts at: 0 1293 1839
Size: 1293 546
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1270 1839
Size: 1270 569
BstBI TT'CG_AA
Cuts at: 0 1830 1839
Size: 1830 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 912 1839
Size: 912 927
BstYI r'GATC_y
Cuts at: 0 831 1839
Size: 831 1008
Bsu36I CC'TnA_GG
Cuts at: 0 10 1839
Size: 10 1829
BtgI C'CryG_G
Cuts at: 0 326 680 1139 1359 1839
Size: 326 354 459 220 480
Fragments arranged by size:
480 459 354 326 220
BtsI GCAGTG_nn'
Cuts at: 0 602 804 809 923 1689 1839
Size: 602 202 5 114 766 150
Fragments arranged by size:
766 602 202 150 114 5
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1776 1811 1839
Size: 1776 35 28
Fragments arranged by size:
1776 35 28
EaeI y'GGCC_r
Cuts at: 0 677 1065 1424 1839
Size: 677 388 359 415
Fragments arranged by size:
677 415 388 359
EagI C'GGCC_G
Cuts at: 0 1065 1839
Size: 1065 774
EarI CTCTTCn'nnn_
Cuts at: 0 1816 1839
Size: 1816 23
EcoICRI GAG'CTC
Cuts at: 0 694 1464 1839
Size: 694 770 375
Fragments arranged by size:
770 694 375
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 710 1342 1556 1839
Size: 442 268 632 214 283
Fragments arranged by size:
632 442 283 268 214
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1538 1839
Size: 1538 301
EcoO109I rG'GnC_Cy
Cuts at: 0 103 104 165 166 210 481 482 498
Size: 103 1 61 1 44 271 1 16
Cuts at: 498 928 1060 1111 1247 1543 1567 1568 1603
Size: 430 132 51 136 296 24 1 35
Cuts at: 1603 1696 1839
Size: 93 143
Fragments arranged by size:
430 296 271 143 136 132 103 93
61 51 44 35 24 16 1 1
1 1
HaeII r_GCGC'y
Cuts at: 0 8 312 1643 1839
Size: 8 304 1331 196
Fragments arranged by size:
1331 304 196 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 816 848 1532 1564 1839
Size: 816 32 684 32 275
Fragments arranged by size:
816 684 275 32 32
HincII GTy'rAC
Cuts at: 0 641 1839
Size: 641 1198
KasI G'GCGC_C
Cuts at: 0 4 1839
Size: 4 1835
KpnI G_GTAC'C
Cuts at: 0 759 1674 1802 1839
Size: 759 915 128 37
Fragments arranged by size:
915 759 128 37
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 1839
Size: 922 917
MslI CAynn'nnrTG
Cuts at: 0 523 887 1138 1393 1839
Size: 523 364 251 255 446
Fragments arranged by size:
523 446 364 255 251
MspA1I CmG'CkG
Cuts at: 0 42 130 422 817 845 912 1083 1214
Size: 42 88 292 395 28 67 171 131
Cuts at: 1214 1361 1433 1485 1667 1839
Size: 147 72 52 182 172
Fragments arranged by size:
395 292 182 172 171 147 131 88
72 67 52 42 28
NaeI GCC'GGC
Cuts at: 0 366 1839
Size: 366 1473
NarI GG'CG_CC
Cuts at: 0 5 1839
Size: 5 1834
NcoI C'CATG_G
Cuts at: 0 1139 1839
Size: 1139 700
NgoMIV G'CCGG_C
Cuts at: 0 364 1839
Size: 364 1475
NheI G'CTAG_C
Cuts at: 0 1338 1839
Size: 1338 501
NsiI A_TGCA'T
Cuts at: 0 1724 1839
Size: 1724 115
NspI r_CATG'y
Cuts at: 0 1300 1657 1722 1726 1839
Size: 1300 357 65 4 113
Fragments arranged by size:
1300 357 113 65 4
PasI CC'CwG_GG
Cuts at: 0 478 952 1839
Size: 478 474 887
Fragments arranged by size:
887 478 474
PciI A'CATG_T
Cuts at: 0 1296 1839
Size: 1296 543
PflMI CCAn_nnn'nTGG
Cuts at: 0 1436 1488 1643 1839
Size: 1436 52 155 196
Fragments arranged by size:
1436 196 155 52
PmlI CAC'GTG
Cuts at: 0 885 1839
Size: 885 954
PpuMI rG'GwC_Cy
Cuts at: 0 1247 1839
Size: 1247 592
PspOMI G'GGCC_C
Cuts at: 0 77 103 165 481 848 1060 1110 1567
Size: 77 26 62 316 367 212 50 457
Cuts at: 1567 1603 1839
Size: 36 236
Fragments arranged by size:
457 367 316 236 212 77 62 50
36 26
PstI C_TGCA'G
Cuts at: 0 353 650 720 1839
Size: 353 297 70 1119
Fragments arranged by size:
1119 353 297 70
PvuI CG_AT'CG
Cuts at: 0 753 1839
Size: 753 1086
PvuII CAG'CTG
Cuts at: 0 130 845 912 1083 1433 1485 1667 1839
Size: 130 715 67 171 350 52 182 172
Fragments arranged by size:
715 350 182 172 171 130 67 52
SacI G_AGCT'C
Cuts at: 0 696 1466 1839
Size: 696 770 373
Fragments arranged by size:
770 696 373
SacII CC_GC'GG
Cuts at: 0 1362 1839
Size: 1362 477
SanDI GG'GwC_CC
Cuts at: 0 1247 1839
Size: 1247 592
SbfI CC_TGCA'GG
Cuts at: 0 353 1839
Size: 353 1486
SfcI C'TryA_G
Cuts at: 0 349 417 646 716 1025 1662 1839
Size: 349 68 229 70 309 637 177
Fragments arranged by size:
637 349 309 229 177 70 68
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 877 1839
Size: 877 962
SfoI GGC'GCC
Cuts at: 0 6 1839
Size: 6 1833
SmaI CCC'GGG
Cuts at: 0 82 1839
Size: 82 1757
SmlI C'TyrA_G
Cuts at: 0 334 1839
Size: 334 1505
SphI G_CATG'C
Cuts at: 0 1657 1726 1839
Size: 1657 69 113
Fragments arranged by size:
1657 113 69
StuI AGG'CCT
Cuts at: 0 1191 1839
Size: 1191 648
StyI C'CwwG_G
Cuts at: 0 242 962 1139 1794 1839
Size: 242 720 177 655 45
Fragments arranged by size:
720 655 242 177 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 763 1428 1480 1635 1839
Size: 763 665 52 155 204
Fragments arranged by size:
763 665 204 155 52
TatI w'GTAC_w
Cuts at: 0 1293 1839
Size: 1293 546
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 373 405 958 990 1337 1369 1839
Size: 373 32 553 32 347 32 470
Fragments arranged by size:
553 470 373 347 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 900 1839
Size: 900 939
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1146 1437 1489 1839
Size: 1146 291 52 350
Fragments arranged by size:
1146 350 291 52
XmaI C'CCGG_G
Cuts at: 0 80 1839
Size: 80 1759
Enzymes that do cut:
AarI AccI Acc65I AflIII AlfI AloI AlwNI ApaI
AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI
BfrBI BglI BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtsI CspCI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII KasI KpnI MmeI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PasI PciI PflMI PmlI PpuMI PspOMI PstI PvuI
PvuII SacI SacII SanDI SbfI SfcI SfiI SfoI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AclI AcuI AfeI AflII AgeI AhdI AleI
ApaLI ApoI AscI AseI AsiSI AvrII BbvCI BcgI
BclI BglII BmgBI BsaI BsaBI BsiWI BsmI BsmBI
BspEI BssHII BssSI BstEII BstZ17I BtgZI ClaI DraI
DraIII DrdI EciI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI MfeI MluI MscI NdeI NotI
NruI PacI PfoI PmeI PpiI PshAI PsiI PspXI
PsrI RsrII SalI SapI ScaI SexAI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI XbaI XhoI XmnI
ZraI