(Linear) (Six-base) MAPSORT of: KIEE2150.seq  Check: 795  from: 1  to: 711



KIEE2150.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 17:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     428     711 
   Size:        428     283
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44     711 
   Size:         44     667
 
AfeI AGC'GCT

Cuts at:      0     604     711 
   Size:        604     107
 
AlwNI CAG_nnn'CTG

Cuts at:      0     468     711 
   Size:        468     243
 
ApoI r'AATT_y

Cuts at:      0      43     618     711 
   Size:         43     575      93

  Fragments arranged by size: 

                 575      93      43
 
BanII G_rGCy'C

Cuts at:      0     500     587     711 
   Size:        500      87     124

  Fragments arranged by size: 

                 500     124      87
 
BclI T'GATC_A

Cuts at:      0     537     711 
   Size:        537     174
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     581     711 
   Size:        581     130
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     327     711 
   Size:        327     384
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     422     711 
   Size:        422     289
 
BsaAI yAC'GTr

Cuts at:      0     580     711 
   Size:        580     131
 
BsaBI GATnn'nnATC

Cuts at:      0     700     711 
   Size:        700      11
 
BsaWI w'CCGG_w

Cuts at:      0     118     711 
   Size:        118     593
 
Bsp1286I G_dGCh'C

Cuts at:      0     500     587     711 
   Size:        500      87     124

  Fragments arranged by size: 

                 500     124      87
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     428     711 
   Size:        428     283
 
BsrDI GCAATG_nn'

Cuts at:      0     535     711 
   Size:        535     176
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     326     711 
   Size:        326     385
 
BstYI r'GATC_y

Cuts at:      0     504     701     711 
   Size:        504     197      10

  Fragments arranged by size: 

                 504     197      10
 
Bsu36I CC'TnA_GG

Cuts at:      0      54     711 
   Size:         54     657
 
BtgI C'CryG_G

Cuts at:      0     290     711 
   Size:        290     421
 
EarI CTCTTCn'nnn_

Cuts at:      0      78     290     437     711 
   Size:         78     212     147     274

  Fragments arranged by size: 

                 274     212     147      78
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44     327     711 
   Size:         44     283     384

  Fragments arranged by size: 

                 384     283      44
 
EcoRI G'AATT_C

Cuts at:      0      43     711 
   Size:         43     668
 
HaeII r_GCGC'y

Cuts at:      0     606     711 
   Size:        606     105
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      87     119     420     452     711 
   Size:         87      32     301      32     259

  Fragments arranged by size: 

                 301     259      87      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     368     711 
   Size:        368     343
 
MslI CAynn'nnrTG

Cuts at:      0     267     578     711 
   Size:        267     311     133

  Fragments arranged by size: 

                 311     267     133
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     569     641     711 
   Size:        569      72      70

  Fragments arranged by size: 

                 569      72      70
 
PmlI CAC'GTG

Cuts at:      0     580     711 
   Size:        580     131
 
PstI C_TGCA'G

Cuts at:      0      81     711 
   Size:         81     630
 
SapI GCTCTTCn'nnn_

Cuts at:      0     437     711 
   Size:        437     274
 
SfcI C'TryA_G

Cuts at:      0      77     711 
   Size:         77     634
 
SmlI C'TyrA_G

Cuts at:      0     437     711 
   Size:        437     274
 
SspI AAT'ATT

Cuts at:      0     214     711 
   Size:        214     497
 
StyI C'CwwG_G

Cuts at:      0      86     711 
   Size:         86     625
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     648     711 
   Size:        648      63
 

 Enzymes that do cut:

     AarI     AcuI     AfeI    AlwNI     ApoI    BanII     BclI     BmrI
     BpmI    BpuEI    BsaAI    BsaBI    BsaWI Bsp1286I    BspMI    BsrDI
    BstXI    BstYI   Bsu36I     BtgI     EarI  Eco57MI    EcoRI    HaeII
    Hin4I     MmeI     MslI    PflMI     PmlI     PstI     SapI     SfcI
     SmlI     SspI     StyI    TaqII

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I     BsaI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI