(Linear) (Six-base) MAPSORT of: KIEE2148.seq  Check: 5212  from: 1  to: 456



KIEE2148.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:55  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361     456 
   Size:        361      95
 
AflIII A'CryG_T

Cuts at:      0     195     456 
   Size:        195     261
 
ApaI G_GGCC'C

Cuts at:      0     357     456 
   Size:        357      99
 
BanII G_rGCy'C

Cuts at:      0     357     456 
   Size:        357      99
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     332     456 
   Size:        332     124
 
BclI T'GATC_A

Cuts at:      0     200     456 
   Size:        200     256
 
BglI GCCn_nnn'nGGC

Cuts at:      0     425     456 
   Size:        425      31
 
Bme1580I G_kGCm'C

Cuts at:      0     357     456 
   Size:        357      99
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     456 
   Size:        352     104
 
Bsp1286I G_dGCh'C

Cuts at:      0     357     456 
   Size:        357      99
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      36     456 
   Size:         36     420
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     306     456 
   Size:        306     150
 
BstYI r'GATC_y

Cuts at:      0     233     456 
   Size:        233     223
 
BtgI C'CryG_G

Cuts at:      0     423     434     456 
   Size:        423      11      22

  Fragments arranged by size: 

                 423      22      11
 
BtsI GCAGTG_nn'

Cuts at:      0      89     456 
   Size:         89     367
 
ClaI AT'CG_AT

Cuts at:      0      59     456 
   Size:         59     397
 
EaeI y'GGCC_r

Cuts at:      0     417     426     456 
   Size:        417       9      30

  Fragments arranged by size: 

                 417      30       9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      84     456 
   Size:         84     372
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     361     456 
   Size:        352       9      95

  Fragments arranged by size: 

                 352      95       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0     291     381     456 
   Size:        291      90      75

  Fragments arranged by size: 

                 291      90      75
 
MslI CAynn'nnrTG

Cuts at:      0     208     456 
   Size:        208     248
 
PpuMI rG'GwC_Cy

Cuts at:      0     291     456 
   Size:        291     165
 
PspOMI G'GGCC_C

Cuts at:      0     353     456 
   Size:        353     103
 
ScaI AGT'ACT

Cuts at:      0      44     456 
   Size:         44     412
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     425     456 
   Size:        425      31
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     427     456 
   Size:        427      29
 
TatI w'GTAC_w

Cuts at:      0      42     227     456 
   Size:         42     185     229

  Fragments arranged by size: 

                 229     185      42
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     102     456 
   Size:        102     354
 

 Enzymes that do cut:

     AcuI   AflIII     ApaI    BanII     BbsI     BclI     BglI Bme1580I
     BpmI Bsp1286I   BstAPI    BstXI    BstYI     BtgI     BtsI     ClaI
     EaeI     EciI  Eco57MI EcoO109I     MslI    PpuMI   PspOMI     ScaI
     SfiI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI    BfrBI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
    CspCI     DraI   DraIII     DrdI     EagI     EarI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfcI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI