(Linear) (Six-base) MAPSORT of: KIEE2147.seq  Check: 8323  from: 1  to: 372



KIEE2147.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 17:11  ..

AgeI A'CCGG_T

Cuts at:      0      35     372 
   Size:         35     337
 
ApoI r'AATT_y

Cuts at:      0     158     372 
   Size:        158     214
 
AvaI C'yCGr_G

Cuts at:      0     245     372 
   Size:        245     127
 
BanI G'GyrC_C

Cuts at:      0      70     372 
   Size:         70     302
 
BanII G_rGCy'C

Cuts at:      0     279     350     372 
   Size:        279      71      22

  Fragments arranged by size: 

                 279      71      22
 
BbeI G_GCGC'C

Cuts at:      0      74     372 
   Size:         74     298
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     372 
   Size:        334      38
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      99     372 
   Size:         99     273
 
BsaHI Gr'CG_yC

Cuts at:      0      71     372 
   Size:         71     301
 
BsaWI w'CCGG_w

Cuts at:      0      35     372 
   Size:         35     337
 
BsiEI CG_ry'CG

Cuts at:      0     104     372 
   Size:        104     268
 
BsiHKAI G_wGCw'C

Cuts at:      0     279     372 
   Size:        279      93
 
Bsp1286I G_dGCh'C

Cuts at:      0     279     350     372 
   Size:        279      71      22

  Fragments arranged by size: 

                 279      71      22
 
BsrFI r'CCGG_y

Cuts at:      0      23      35     325     372 
   Size:         23      12     290      47

  Fragments arranged by size: 

                 290      47      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      25     275     372 
   Size:         25     250      97

  Fragments arranged by size: 

                 250      97      25
 
EaeI y'GGCC_r

Cuts at:      0     126     282     372 
   Size:        126     156      90

  Fragments arranged by size: 

                 156     126      90
 
EcoICRI GAG'CTC

Cuts at:      0     277     372 
   Size:        277      95
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     372 
   Size:        334      38
 
EcoRI G'AATT_C

Cuts at:      0     158     372 
   Size:        158     214
 
HaeII r_GCGC'y

Cuts at:      0      74     372 
   Size:         74     298
 
KasI G'GCGC_C

Cuts at:      0      70     372 
   Size:         70     302
 
MscI TGG'CCA

Cuts at:      0     128     284     372 
   Size:        128     156      88

  Fragments arranged by size: 

                 156     128      88
 
NaeI GCC'GGC

Cuts at:      0      25     372 
   Size:         25     347
 
NarI GG'CG_CC

Cuts at:      0      71     372 
   Size:         71     301
 
NgoMIV G'CCGG_C

Cuts at:      0      23     372 
   Size:         23     349
 
NspI r_CATG'y

Cuts at:      0     335     372 
   Size:        335      37
 
PasI CC'CwG_GG

Cuts at:      0      66     372 
   Size:         66     306
 
SacI G_AGCT'C

Cuts at:      0     279     372 
   Size:        279      93
 
SfcI C'TryA_G

Cuts at:      0     150     372 
   Size:        150     222
 
SfoI GGC'GCC

Cuts at:      0      72     372 
   Size:         72     300
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     302     372 
   Size:        302      70
 

 Enzymes that do cut:

     AgeI     ApoI     AvaI     BanI    BanII     BbeI     BpmI     BsaI
    BsaHI    BsaWI    BsiEI  BsiHKAI Bsp1286I    BsrFI    BtgZI     EaeI
  EcoICRI  Eco57MI    EcoRI    HaeII     KasI     MscI     NaeI     NarI
   NgoMIV     NspI     PasI     SacI     SfcI     SfoI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaXI    BseRI    BseYI     BsgI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI