Restriction map for
pFN21AE2134
(Linear) (Six-base) MAPSORT of: KIEE2134.seq Check: 6225 from: 1 to: 2574
KIEE2134.orf
With 182 enzymes: SgfI *
December 5, 2014 12:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2321 2574
Size: 2321 253
AccI GT'mk_AC
Cuts at: 0 241 2574
Size: 241 2333
Acc65I G'GTAC_C
Cuts at: 0 1338 2574
Size: 1338 1236
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 1391 2079 2298 2574
Size: 255 1136 688 219 276
Fragments arranged by size:
1136 688 276 255 219
AleI CACnn'nnGTG
Cuts at: 0 2116 2574
Size: 2116 458
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 353 2574
Size: 353 2221
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1418 1450 2574
Size: 1418 32 1124
Fragments arranged by size:
1418 1124 32
AlwNI CAG_nnn'CTG
Cuts at: 0 184 222 351 2028 2062 2574
Size: 184 38 129 1677 34 512
Fragments arranged by size:
1677 512 184 129 38 34
ApoI r'AATT_y
Cuts at: 0 59 426 507 1062 1141 2574
Size: 59 367 81 555 79 1433
Fragments arranged by size:
1433 555 367 81 79 59
AvaI C'yCGr_G
Cuts at: 0 229 568 2574
Size: 229 339 2006
Fragments arranged by size:
2006 339 229
AvrII C'CTAG_G
Cuts at: 0 24 2574
Size: 24 2550
BanI G'GyrC_C
Cuts at: 0 796 1338 2300 2574
Size: 796 542 962 274
Fragments arranged by size:
962 796 542 274
BanII G_rGCy'C
Cuts at: 0 230 569 575 1478 2574
Size: 230 339 6 903 1096
Fragments arranged by size:
1096 903 339 230 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 891 2574
Size: 891 1683
BlpI GC'TnA_GC
Cuts at: 0 157 2574
Size: 157 2417
Bme1580I G_kGCm'C
Cuts at: 0 2056 2574
Size: 2056 518
BmrI ACTGGGnnnn_n'
Cuts at: 0 654 2382 2574
Size: 654 1728 192
Fragments arranged by size:
1728 654 192
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 52 2574
Size: 52 2522
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 703 1333 2449 2574
Size: 244 459 630 1116 125
Fragments arranged by size:
1116 630 459 244 125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 1248 2574
Size: 916 332 1326
Fragments arranged by size:
1326 916 332
BsaI GGTCTCn'nnnn_
Cuts at: 0 394 2574
Size: 394 2180
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1331 1361 2574
Size: 1331 30 1213
Fragments arranged by size:
1331 1213 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1371 2574
Size: 1371 1203
BseYI C'CCAG_C
Cuts at: 0 1417 1804 2574
Size: 1417 387 770
Fragments arranged by size:
1417 770 387
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2140 2574
Size: 2140 434
BsiHKAI G_wGCw'C
Cuts at: 0 38 230 569 1478 2358 2574
Size: 38 192 339 909 880 216
Fragments arranged by size:
909 880 339 216 192 38
BsmI GAATG_Cn'
Cuts at: 0 2130 2574
Size: 2130 444
Bsp1286I G_dGCh'C
Cuts at: 0 38 230 569 575 1478 2056 2358 2574
Size: 38 192 339 6 903 578 302 216
Fragments arranged by size:
903 578 339 302 216 192 38 6
BspHI T'CATG_A
Cuts at: 0 1961 2574
Size: 1961 613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 287 1647 2321 2574
Size: 287 1360 674 253
Fragments arranged by size:
1360 674 287 253
BsrDI GCAATG_nn'
Cuts at: 0 747 1112 2219 2574
Size: 747 365 1107 355
Fragments arranged by size:
1107 747 365 355
BsrFI r'CCGG_y
Cuts at: 0 43 2574
Size: 43 2531
BsrGI T'GTAC_A
Cuts at: 0 2069 2427 2574
Size: 2069 358 147
Fragments arranged by size:
2069 358 147
BssSI C'ACGA_G
Cuts at: 0 914 2574
Size: 914 1660
BstBI TT'CG_AA
Cuts at: 0 1060 2574
Size: 1060 1514
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2473 2574
Size: 2473 101
BstYI r'GATC_y
Cuts at: 0 450 825 879 1167 1652 2460 2574
Size: 450 375 54 288 485 808 114
Fragments arranged by size:
808 485 450 375 288 114 54
BstZ17I GTA'TAC
Cuts at: 0 242 2574
Size: 242 2332
Bsu36I CC'TnA_GG
Cuts at: 0 1079 2456 2574
Size: 1079 1377 118
Fragments arranged by size:
1377 1079 118
BtgI C'CryG_G
Cuts at: 0 1874 2574
Size: 1874 700
BtsI GCAGTG_nn'
Cuts at: 0 182 2574
Size: 182 2392
DraI TTT'AAA
Cuts at: 0 431 1364 2574
Size: 431 933 1210
Fragments arranged by size:
1210 933 431
DraIII CAC_nnn'GTG
Cuts at: 0 1714 2574
Size: 1714 860
EaeI y'GGCC_r
Cuts at: 0 41 81 854 2574
Size: 41 40 773 1720
Fragments arranged by size:
1720 773 41 40
EarI CTCTTCn'nnn_
Cuts at: 0 1867 1886 2574
Size: 1867 19 688
Fragments arranged by size:
1867 688 19
EcoICRI GAG'CTC
Cuts at: 0 228 567 1476 2574
Size: 228 339 909 1098
Fragments arranged by size:
1098 909 339 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 255 703 1333 1391 2079 2298 2449
Size: 244 11 448 630 58 688 219 151
Cuts at: 2449 2574
Size: 125
Fragments arranged by size:
688 630 448 244 219 151 125 58
11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 115 2574
Size: 115 2459
EcoO109I rG'GnC_Cy
Cuts at: 0 120 129 2378 2574
Size: 120 9 2249 196
Fragments arranged by size:
2249 196 120 9
EcoRI G'AATT_C
Cuts at: 0 59 507 2574
Size: 59 448 2067
Fragments arranged by size:
2067 448 59
EcoRV GAT'ATC
Cuts at: 0 1221 2574
Size: 1221 1353
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 285 339 2574
Size: 285 54 2235
Fragments arranged by size:
2235 285 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 517 549 1152 1184 1580 1612 1831 1863
Size: 517 32 603 32 396 32 219 32
Cuts at: 1863 2186 2218 2250 2282 2391 2423 2574
Size: 323 32 32 32 109 32 151
Fragments arranged by size:
603 517 396 323 219 151 109 32
32 32 32 32 32 32 32
KpnI G_GTAC'C
Cuts at: 0 1342 2574
Size: 1342 1232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 1628 2574
Size: 560 1068 946
Fragments arranged by size:
1068 946 560
MscI TGG'CCA
Cuts at: 0 856 2574
Size: 856 1718
MslI CAynn'nnrTG
Cuts at: 0 335 1790 2116 2365 2368 2574
Size: 335 1455 326 249 3 206
Fragments arranged by size:
1455 335 326 249 206 3
MspA1I CmG'CkG
Cuts at: 0 213 219 2574
Size: 213 6 2355
Fragments arranged by size:
2355 213 6
PasI CC'CwG_GG
Cuts at: 0 2049 2325 2574
Size: 2049 276 249
Fragments arranged by size:
2049 276 249
PflMI CCAn_nnn'nTGG
Cuts at: 0 792 2028 2574
Size: 792 1236 546
Fragments arranged by size:
1236 792 546
PfoI T'CCnGG_A
Cuts at: 0 1145 1655 1666 2231 2465 2574
Size: 1145 510 11 565 234 109
Fragments arranged by size:
1145 565 510 234 109 11
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 551 583 2574
Size: 551 32 1991
Fragments arranged by size:
1991 551 32
PpuMI rG'GwC_Cy
Cuts at: 0 120 129 2378 2574
Size: 120 9 2249 196
Fragments arranged by size:
2249 196 120 9
PsiI TTA'TAA
Cuts at: 0 2012 2574
Size: 2012 562
PspXI vC'TCGA_Gb
Cuts at: 0 568 2574
Size: 568 2006
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 943 975 2574
Size: 943 32 1599
Fragments arranged by size:
1599 943 32
PstI C_TGCA'G
Cuts at: 0 218 1818 2574
Size: 218 1600 756
Fragments arranged by size:
1600 756 218
PvuII CAG'CTG
Cuts at: 0 213 219 2574
Size: 213 6 2355
Fragments arranged by size:
2355 213 6
SacI G_AGCT'C
Cuts at: 0 230 569 1478 2574
Size: 230 339 909 1096
Fragments arranged by size:
1096 909 339 230
SfcI C'TryA_G
Cuts at: 0 214 1386 1799 1814 2558 2574
Size: 214 1172 413 15 744 16
Fragments arranged by size:
1172 744 413 214 16 15
SmlI C'TyrA_G
Cuts at: 0 568 931 1227 2574
Size: 568 363 296 1347
Fragments arranged by size:
1347 568 363 296
SpeI A'CTAG_T
Cuts at: 0 1242 2574
Size: 1242 1332
SspI AAT'ATT
Cuts at: 0 545 2574
Size: 545 2029
StuI AGG'CCT
Cuts at: 0 268 763 2574
Size: 268 495 1811
Fragments arranged by size:
1811 495 268
StyI C'CwwG_G
Cuts at: 0 24 1163 1437 2574
Size: 24 1139 274 1137
Fragments arranged by size:
1139 1137 274 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 130 784 2106 2574
Size: 130 654 1322 468
Fragments arranged by size:
1322 654 468 130
TatI w'GTAC_w
Cuts at: 0 2069 2427 2574
Size: 2069 358 147
Fragments arranged by size:
2069 358 147
Tth111I GACn'n_nGTC
Cuts at: 0 2200 2574
Size: 2200 374
XbaI T'CTAG_A
Cuts at: 0 1311 2574
Size: 1311 1263
XhoI C'TCGA_G
Cuts at: 0 568 2574
Size: 568 2006
XmnI GAAnn'nnTTC
Cuts at: 0 63 664 2574
Size: 63 601 1910
Fragments arranged by size:
1910 601 63
Enzymes that do cut:
AarI AccI Acc65I AcuI AleI AlfI AloI AlwNI
ApoI AvaI AvrII BanI BanII BbsI BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI
BsrGI BssSI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtsI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI Hin4I KpnI MmeI MscI
MslI MspA1I PasI PflMI PfoI PpiI PpuMI PsiI
PspXI PsrI PstI PvuII SacI SfcI SmlI SpeI
SspI StuI StyI TaqII TatI Tth111I XbaI XhoI
XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI ApaI
ApaLI AscI AseI AsiSI BaeI BamHI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BmgBI BmtI
Bpu10I BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI
BspEI BsrBI BssHII BstAPI BstEII BtgZI ClaI CspCI
DrdI EagI EciI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PshAI PspOMI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI SwaI TstI
XcmI XmaI ZraI