Restriction map for
pFN21AE2125
(Linear) (Six-base) MAPSORT of: KIEE2125.seq Check: 160 from: 1 to: 984
KIEE2125.orf
With 182 enzymes: SgfI *
July 3, 2012 17:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 606 984
Size: 606 378
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 892 984
Size: 586 306 92
Fragments arranged by size:
586 306 92
AlwNI CAG_nnn'CTG
Cuts at: 0 261 598 984
Size: 261 337 386
Fragments arranged by size:
386 337 261
ApoI r'AATT_y
Cuts at: 0 382 984
Size: 382 602
AvaI C'yCGr_G
Cuts at: 0 466 718 984
Size: 466 252 266
Fragments arranged by size:
466 266 252
BamHI G'GATC_C
Cuts at: 0 45 984
Size: 45 939
BanII G_rGCy'C
Cuts at: 0 141 984
Size: 141 843
BbsI GAAGACnn'nnnn_
Cuts at: 0 227 466 984
Size: 227 239 518
Fragments arranged by size:
518 239 227
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 457 491 984
Size: 457 34 493
Fragments arranged by size:
493 457 34
BglII A'GATC_T
Cuts at: 0 423 984
Size: 423 561
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 476 667 984
Size: 84 392 191 317
Fragments arranged by size:
392 317 191 84
Bpu10I CC'TnA_GC
Cuts at: 0 140 821 984
Size: 140 681 163
Fragments arranged by size:
681 163 140
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 984
Size: 358 626
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 511 900 984
Size: 511 389 84
Fragments arranged by size:
511 389 84
BseYI C'CCAG_C
Cuts at: 0 794 984
Size: 794 190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 984
Size: 862 122
BsmBI CGTCTCn'nnnn_
Cuts at: 0 604 984
Size: 604 380
Bsp1286I G_dGCh'C
Cuts at: 0 141 984
Size: 141 843
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 606 984
Size: 606 378
BssHII G'CGCG_C
Cuts at: 0 761 984
Size: 761 223
BstXI CCAn_nnnn'nTGG
Cuts at: 0 555 984
Size: 555 429
BstYI r'GATC_y
Cuts at: 0 45 399 423 984
Size: 45 354 24 561
Fragments arranged by size:
561 354 45 24
Bsu36I CC'TnA_GG
Cuts at: 0 689 851 984
Size: 689 162 133
Fragments arranged by size:
689 162 133
BtgI C'CryG_G
Cuts at: 0 282 984
Size: 282 702
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 488 984
Size: 488 496
BtsI GCAGTG_nn'
Cuts at: 0 56 984
Size: 56 928
EaeI y'GGCC_r
Cuts at: 0 87 263 746 984
Size: 87 176 483 238
Fragments arranged by size:
483 238 176 87
EarI CTCTTCn'nnn_
Cuts at: 0 751 767 984
Size: 751 16 217
Fragments arranged by size:
751 217 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 39 984
Size: 39 945
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 476 586 667 892 984
Size: 84 392 110 81 225 92
Fragments arranged by size:
392 225 110 92 84 81
EcoO109I rG'GnC_Cy
Cuts at: 0 787 984
Size: 787 197
HaeII r_GCGC'y
Cuts at: 0 524 984
Size: 524 460
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 194 222 226 254 331 363 391 423
Size: 194 28 4 28 77 32 28 32
Cuts at: 423 984
Size: 561
Fragments arranged by size:
561 194 77 32 32 28 28 28
4
MscI TGG'CCA
Cuts at: 0 89 748 984
Size: 89 659 236
Fragments arranged by size:
659 236 89
MslI CAynn'nnrTG
Cuts at: 0 242 984
Size: 242 742
MspA1I CmG'CkG
Cuts at: 0 93 984
Size: 93 891
NcoI C'CATG_G
Cuts at: 0 282 984
Size: 282 702
PflMI CCAn_nnn'nTGG
Cuts at: 0 261 984
Size: 261 723
PfoI T'CCnGG_A
Cuts at: 0 533 984
Size: 533 451
SapI GCTCTTCn'nnn_
Cuts at: 0 751 767 984
Size: 751 16 217
Fragments arranged by size:
751 217 16
ScaI AGT'ACT
Cuts at: 0 541 984
Size: 541 443
SmlI C'TyrA_G
Cuts at: 0 337 984
Size: 337 647
StuI AGG'CCT
Cuts at: 0 814 984
Size: 814 170
StyI C'CwwG_G
Cuts at: 0 282 809 984
Size: 282 527 175
Fragments arranged by size:
527 282 175
TatI w'GTAC_w
Cuts at: 0 539 984
Size: 539 445
Enzymes that do cut:
AarI AcuI AlwNI ApoI AvaI BamHI BanII BbsI
BcgI BglII BpmI Bpu10I BpuEI BseRI BseYI BsgI
BsmBI Bsp1286I BspMI BssHII BstXI BstYI Bsu36I BtgI
BtgZI BtsI EaeI EarI EciI Eco57MI EcoO109I HaeII
Hin4I MscI MslI MspA1I NcoI PflMI PfoI SapI
ScaI SmlI StuI StyI TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BanI BbeI BbvCI BciVI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstZ17I ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI