Restriction map for
pFN21AE2123
(Linear) (Six-base) MAPSORT of: KIEE2123.seq Check: 5561 from: 1 to: 2742
KIEE2123.orf
With 182 enzymes: SgfI *
February 3, 2015 16:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1348 2373 2742
Size: 1348 1025 369
Fragments arranged by size:
1348 1025 369
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1510 2593 2659 2742
Size: 1510 1083 66 83
Fragments arranged by size:
1510 1083 83 66
AfeI AGC'GCT
Cuts at: 0 283 1999 2329 2742
Size: 283 1716 330 413
Fragments arranged by size:
1716 413 330 283
AflIII A'CryG_T
Cuts at: 0 2435 2742
Size: 2435 307
AleI CACnn'nnGTG
Cuts at: 0 2302 2742
Size: 2302 440
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 463 495 2194 2226 2742
Size: 463 32 1699 32 516
Fragments arranged by size:
1699 516 463 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 468 772 1542 2742
Size: 468 304 770 1200
Fragments arranged by size:
1200 770 468 304
ApaLI G'TGCA_C
Cuts at: 0 2125 2742
Size: 2125 617
AvaI C'yCGr_G
Cuts at: 0 289 817 839 1345 2742
Size: 289 528 22 506 1397
Fragments arranged by size:
1397 528 506 289 22
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2382 2415 2742
Size: 2382 33 327
Fragments arranged by size:
2382 327 33
BamHI G'GATC_C
Cuts at: 0 1023 1421 2742
Size: 1023 398 1321
Fragments arranged by size:
1321 1023 398
BanI G'GyrC_C
Cuts at: 0 43 384 604 1215 1278 1606 1684 2742
Size: 43 341 220 611 63 328 78 1058
Fragments arranged by size:
1058 611 341 328 220 78 63 43
BanII G_rGCy'C
Cuts at: 0 67 187 818 944 1237 1650 1940 2033
Size: 67 120 631 126 293 413 290 93
Cuts at: 2033 2345 2742
Size: 312 397
Fragments arranged by size:
631 413 397 312 293 290 126 120
93 67
BbeI G_GCGC'C
Cuts at: 0 47 1282 2742
Size: 47 1235 1460
Fragments arranged by size:
1460 1235 47
BbsI GAAGACnn'nnnn_
Cuts at: 0 1501 1511 2589 2742
Size: 1501 10 1078 153
Fragments arranged by size:
1501 1078 153 10
BbvCI CC'TCA_GC
Cuts at: 0 2092 2286 2742
Size: 2092 194 456
Fragments arranged by size:
2092 456 194
BclI T'GATC_A
Cuts at: 0 212 2742
Size: 212 2530
BglI GCCn_nnn'nGGC
Cuts at: 0 25 511 1092 2742
Size: 25 486 581 1650
Fragments arranged by size:
1650 581 486 25
BglII A'GATC_T
Cuts at: 0 1161 1550 2742
Size: 1161 389 1192
Fragments arranged by size:
1192 1161 389
BlpI GC'TnA_GC
Cuts at: 0 1897 2119 2259 2742
Size: 1897 222 140 483
Fragments arranged by size:
1897 483 222 140
Bme1580I G_kGCm'C
Cuts at: 0 607 723 1220 1609 1687 1705 2129 2742
Size: 607 116 497 389 78 18 424 613
Fragments arranged by size:
613 607 497 424 389 116 78 18
BmgBI CAC'GTC
Cuts at: 0 1499 2154 2421 2742
Size: 1499 655 267 321
Fragments arranged by size:
1499 655 321 267
BmrI ACTGGGnnnn_n'
Cuts at: 0 847 1299 2742
Size: 847 452 1443
Fragments arranged by size:
1443 847 452
BmtI G_CTAG'C
Cuts at: 0 826 2742
Size: 826 1916
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1572 1626 2742
Size: 1572 54 1116
Fragments arranged by size:
1572 1116 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 382 730 763 794 848 884 908
Size: 124 258 348 33 31 54 36 24
Cuts at: 908 928 938 968 998 1046 1072 1142 1648
Size: 20 10 30 30 48 26 70 506
Cuts at: 1648 1954 2022 2250 2337 2533 2742
Size: 306 68 228 87 196 209
Fragments arranged by size:
506 348 306 258 228 209 196 124
87 70 68 54 48 36 33 31
30 30 26 24 20 10
Bpu10I CC'TnA_GC
Cuts at: 0 2074 2092 2286 2742
Size: 2074 18 194 456
Fragments arranged by size:
2074 456 194 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 1411 2704 2742
Size: 646 765 1293 38
Fragments arranged by size:
1293 765 646 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 2236 2742
Size: 2236 506
BsaAI yAC'GTr
Cuts at: 0 681 2742
Size: 681 2061
BsaBI GATnn'nnATC
Cuts at: 0 2167 2742
Size: 2167 575
BsaHI Gr'CG_yC
Cuts at: 0 44 92 113 503 1279 2742
Size: 44 48 21 390 776 1463
Fragments arranged by size:
1463 776 390 48 44 21
BsaWI w'CCGG_w
Cuts at: 0 645 936 1526 2564 2742
Size: 645 291 590 1038 178
Fragments arranged by size:
1038 645 590 291 178
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 220 250 882 912 2341 2371 2654 2684
Size: 220 30 632 30 1429 30 283 30
Cuts at: 2684 2742
Size: 58
Fragments arranged by size:
1429 632 283 220 58 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 220 507 582 1144 1797 2130 2173 2274
Size: 220 287 75 562 653 333 43 101
Cuts at: 2274 2530 2533 2536 2742
Size: 256 3 3 206
Fragments arranged by size:
653 562 333 287 256 220 206 101
75 43 3 3
BseYI C'CCAG_C
Cuts at: 0 737 847 1247 2020 2742
Size: 737 110 400 773 722
Fragments arranged by size:
773 737 722 400 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 124 241 271 703 2742
Size: 25 99 117 30 432 2039
Fragments arranged by size:
2039 432 117 99 30 25
BsiEI CG_ry'CG
Cuts at: 0 29 1660 2010 2135 2742
Size: 29 1631 350 125 607
Fragments arranged by size:
1631 607 350 125 29
BsiHKAI G_wGCw'C
Cuts at: 0 187 380 749 818 846 944 1274 1394
Size: 187 193 369 69 28 98 330 120
Cuts at: 1394 2033 2129 2742
Size: 639 96 613
Fragments arranged by size:
639 613 369 330 193 187 120 98
96 69 28
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1660 2742
Size: 1660 1082
Bsp1286I G_dGCh'C
Cuts at: 0 67 187 380 607 723 749 818 846
Size: 67 120 193 227 116 26 69 28
Cuts at: 846 944 1220 1237 1274 1394 1609 1650 1687
Size: 98 276 17 37 120 215 41 37
Cuts at: 1687 1705 1940 2033 2129 2345 2742
Size: 18 235 93 96 216 397
Fragments arranged by size:
397 276 235 227 216 215 193 120
120 116 98 96 93 69 67 41
37 37 28 26 18 17
BspEI T'CCGG_A
Cuts at: 0 645 936 1526 2742
Size: 645 291 590 1216
Fragments arranged by size:
1216 645 590 291
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 176 482 1348 2373 2742
Size: 176 306 866 1025 369
Fragments arranged by size:
1025 866 369 306 176
BsrBI CCG'CTC
Cuts at: 0 1893 2370 2742
Size: 1893 477 372
Fragments arranged by size:
1893 477 372
BsrFI r'CCGG_y
Cuts at: 0 858 2134 2742
Size: 858 1276 608
Fragments arranged by size:
1276 858 608
BsrGI T'GTAC_A
Cuts at: 0 2438 2742
Size: 2438 304
BstAPI GCAn_nnn'nTGC
Cuts at: 0 384 2742
Size: 384 2358
BstEII G'GTnAC_C
Cuts at: 0 2454 2742
Size: 2454 288
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1386 2304 2742
Size: 1386 918 438
Fragments arranged by size:
1386 918 438
BstYI r'GATC_y
Cuts at: 0 1023 1161 1421 1529 1550 2576 2742
Size: 1023 138 260 108 21 1026 166
Fragments arranged by size:
1026 1023 260 166 138 108 21
Bsu36I CC'TnA_GG
Cuts at: 0 773 1966 2742
Size: 773 1193 776
Fragments arranged by size:
1193 776 773
BtgI C'CryG_G
Cuts at: 0 261 1886 2303 2495 2742
Size: 261 1625 417 192 247
Fragments arranged by size:
1625 417 261 247 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1833 2091 2742
Size: 1833 258 651
Fragments arranged by size:
1833 651 258
BtsI GCAGTG_nn'
Cuts at: 0 1224 1733 2742
Size: 1224 509 1009
Fragments arranged by size:
1224 1009 509
DraIII CAC_nnn'GTG
Cuts at: 0 384 2742
Size: 384 2358
EaeI y'GGCC_r
Cuts at: 0 26 1319 1406 1657 2132 2196 2742
Size: 26 1293 87 251 475 64 546
Fragments arranged by size:
1293 546 475 251 87 64 26
EagI C'GGCC_G
Cuts at: 0 26 1657 2132 2742
Size: 26 1631 475 610
Fragments arranged by size:
1631 610 475 26
EarI CTCTTCn'nnn_
Cuts at: 0 1774 2742
Size: 1774 968
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 87 2742
Size: 87 2655
EcoICRI GAG'CTC
Cuts at: 0 185 816 942 2031 2742
Size: 185 631 126 1089 711
Fragments arranged by size:
1089 711 631 185 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 382 730 763 794 848 884 908
Size: 124 258 348 33 31 54 36 24
Cuts at: 908 928 938 968 998 1046 1072 1142 1510
Size: 20 10 30 30 48 26 70 368
Cuts at: 1510 1648 1954 2022 2250 2337 2533 2593 2659
Size: 138 306 68 228 87 196 60 66
Cuts at: 2659 2742
Size: 83
Fragments arranged by size:
368 348 306 258 228 196 138 124
87 83 70 68 66 60 54 48
36 33 31 30 30 26 24 20
10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 244 2515 2742
Size: 244 2271 227
Fragments arranged by size:
2271 244 227
EcoO109I rG'GnC_Cy
Cuts at: 0 465 1871 2016 2742
Size: 465 1406 145 726
Fragments arranged by size:
1406 726 465 145
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2667 2742
Size: 2667 75
FspI TGC'GCA
Cuts at: 0 244 2742
Size: 244 2498
HaeII r_GCGC'y
Cuts at: 0 47 285 717 991 1069 1282 2001 2331
Size: 47 238 432 274 78 213 719 330
Cuts at: 2331 2742
Size: 411
Fragments arranged by size:
719 432 411 330 274 238 213 78
47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 289 321 607 639 766 798 1927 1959
Size: 289 32 286 32 127 32 1129 32
Cuts at: 1959 2654 2686 2742
Size: 695 32 56
Fragments arranged by size:
1129 695 289 286 127 56 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 640 2742
Size: 640 2102
KasI G'GCGC_C
Cuts at: 0 43 1278 2742
Size: 43 1235 1464
Fragments arranged by size:
1464 1235 43
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 913 2174 2742
Size: 572 341 1261 568
Fragments arranged by size:
1261 572 568 341
MscI TGG'CCA
Cuts at: 0 1321 1408 2198 2742
Size: 1321 87 790 544
Fragments arranged by size:
1321 790 544 87
MslI CAynn'nnrTG
Cuts at: 0 1738 2302 2452 2742
Size: 1738 564 150 290
Fragments arranged by size:
1738 564 290 150
MspA1I CmG'CkG
Cuts at: 0 54 125 255 669 741 964 1251 2099
Size: 54 71 130 414 72 223 287 848
Cuts at: 2099 2742
Size: 643
Fragments arranged by size:
848 643 414 287 223 130 72 71
54
NaeI GCC'GGC
Cuts at: 0 860 2136 2742
Size: 860 1276 606
Fragments arranged by size:
1276 860 606
NarI GG'CG_CC
Cuts at: 0 44 1279 2742
Size: 44 1235 1463
Fragments arranged by size:
1463 1235 44
NcoI C'CATG_G
Cuts at: 0 2495 2742
Size: 2495 247
NgoMIV G'CCGG_C
Cuts at: 0 858 2134 2742
Size: 858 1276 608
Fragments arranged by size:
1276 858 608
NheI G'CTAG_C
Cuts at: 0 822 2742
Size: 822 1920
NspI r_CATG'y
Cuts at: 0 15 696 2439 2742
Size: 15 681 1743 303
Fragments arranged by size:
1743 681 303 15
PciI A'CATG_T
Cuts at: 0 2435 2742
Size: 2435 307
PflMI CCAn_nnn'nTGG
Cuts at: 0 1230 2385 2742
Size: 1230 1155 357
Fragments arranged by size:
1230 1155 357
PfoI T'CCnGG_A
Cuts at: 0 467 2180 2742
Size: 467 1713 562
Fragments arranged by size:
1713 562 467
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1558 1590 2742
Size: 1558 32 1152
Fragments arranged by size:
1558 1152 32
PpuMI rG'GwC_Cy
Cuts at: 0 465 2742
Size: 465 2277
PspXI vC'TCGA_Gb
Cuts at: 0 817 839 2742
Size: 817 22 1903
Fragments arranged by size:
1903 817 22
PstI C_TGCA'G
Cuts at: 0 1469 1826 2549 2742
Size: 1469 357 723 193
Fragments arranged by size:
1469 723 357 193
PvuII CAG'CTG
Cuts at: 0 125 255 669 741 964 2742
Size: 125 130 414 72 223 1778
Fragments arranged by size:
1778 414 223 130 125 72
RsrII CG'GwC_CG
Cuts at: 0 341 2742
Size: 341 2401
SacI G_AGCT'C
Cuts at: 0 187 818 944 2033 2742
Size: 187 631 126 1089 709
Fragments arranged by size:
1089 709 631 187 126
ScaI AGT'ACT
Cuts at: 0 622 2742
Size: 622 2120
SexAI A'CCwGG_T
Cuts at: 0 380 1718 2742
Size: 380 1338 1024
Fragments arranged by size:
1338 1024 380
SfcI C'TryA_G
Cuts at: 0 438 1465 1822 1842 2545 2742
Size: 438 1027 357 20 703 197
Fragments arranged by size:
1027 703 438 357 197 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 25 2742
Size: 25 2717
SfoI GGC'GCC
Cuts at: 0 45 1280 2742
Size: 45 1235 1462
Fragments arranged by size:
1462 1235 45
SmlI C'TyrA_G
Cuts at: 0 661 817 839 1426 2719 2742
Size: 661 156 22 587 1293 23
Fragments arranged by size:
1293 661 587 156 23 22
StyI C'CwwG_G
Cuts at: 0 2495 2657 2729 2742
Size: 2495 162 72 13
Fragments arranged by size:
2495 162 72 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 472 861 1318 2335 2742
Size: 472 389 457 1017 407
Fragments arranged by size:
1017 472 457 407 389
TatI w'GTAC_w
Cuts at: 0 620 2438 2742
Size: 620 1818 304
Fragments arranged by size:
1818 620 304
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2044 2076 2742
Size: 2044 32 666
Fragments arranged by size:
2044 666 32
Tth111I GACn'n_nGTC
Cuts at: 0 2632 2742
Size: 2632 110
XbaI T'CTAG_A
Cuts at: 0 520 1044 2742
Size: 520 524 1698
Fragments arranged by size:
1698 524 520
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2077 2301 2742
Size: 2077 224 441
Fragments arranged by size:
2077 441 224
XhoI C'TCGA_G
Cuts at: 0 817 839 2742
Size: 817 22 1903
Fragments arranged by size:
1903 817 22
XmnI GAAnn'nnTTC
Cuts at: 0 1792 2742
Size: 1792 950
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AloI AlwNI ApaLI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI
PflMI PfoI PpiI PpuMI PspXI PstI PvuII RsrII
SacI ScaI SexAI SfcI SfiI SfoI SmlI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AlfI
ApaI ApoI AscI AseI AsiSI AvrII BcgI BciVI
BfrBI BsiWI BsmI BspHI BsrDI BssHII BssSI BstBI
BstZ17I ClaI CspCI DraI DrdI EcoRI EcoRV FseI
FspAI HindIII HpaI KpnI MfeI MluI NdeI NotI
NruI NsiI PacI PasI PmeI PmlI PshAI PsiI
PspOMI PsrI PvuI SacII SalI SanDI SapI SbfI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI XmaI ZraI