Restriction map for
pFN21AE2122
(Linear) (Six-base) MAPSORT of: KIEE2122.seq Check: 7118 from: 1 to: 2715
KIEE2122.orf
With 182 enzymes: SgfI *
July 3, 2012 17:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2046 2715
Size: 2046 669
AatII G_ACGT'C
Cuts at: 0 1451 2715
Size: 1451 1264
AclI AA'CG_TT
Cuts at: 0 1796 2715
Size: 1796 919
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 658 2642 2715
Size: 610 48 1984 73
Fragments arranged by size:
1984 610 73 48
AfeI AGC'GCT
Cuts at: 0 944 2715
Size: 944 1771
AleI CACnn'nnGTG
Cuts at: 0 1513 2715
Size: 1513 1202
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 530 1027 2715
Size: 530 497 1688
Fragments arranged by size:
1688 530 497
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1807 1839 2715
Size: 1807 32 876
Fragments arranged by size:
1807 876 32
AlwNI CAG_nnn'CTG
Cuts at: 0 588 1032 2403 2548 2715
Size: 588 444 1371 145 167
Fragments arranged by size:
1371 588 444 167 145
ApoI r'AATT_y
Cuts at: 0 207 2626 2692 2715
Size: 207 2419 66 23
Fragments arranged by size:
2419 207 66 23
AseI AT'TA_AT
Cuts at: 0 2225 2715
Size: 2225 490
AvaI C'yCGr_G
Cuts at: 0 626 1643 2715
Size: 626 1017 1072
Fragments arranged by size:
1072 1017 626
BamHI G'GATC_C
Cuts at: 0 594 2715
Size: 594 2121
BanI G'GyrC_C
Cuts at: 0 361 2715
Size: 361 2354
BanII G_rGCy'C
Cuts at: 0 636 2417 2715
Size: 636 1781 298
Fragments arranged by size:
1781 636 298
BbsI GAAGACnn'nnnn_
Cuts at: 0 582 1443 2715
Size: 582 861 1272
Fragments arranged by size:
1272 861 582
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 811 845 2715
Size: 811 34 1870
Fragments arranged by size:
1870 811 34
BclI T'GATC_A
Cuts at: 0 1886 2159 2289 2715
Size: 1886 273 130 426
Fragments arranged by size:
1886 426 273 130
BglI GCCn_nnn'nGGC
Cuts at: 0 631 812 1300 2014 2715
Size: 631 181 488 714 701
Fragments arranged by size:
714 701 631 488 181
BglII A'GATC_T
Cuts at: 0 249 1122 2715
Size: 249 873 1593
Fragments arranged by size:
1593 873 249
BlpI GC'TnA_GC
Cuts at: 0 1036 2051 2715
Size: 1036 1015 664
Fragments arranged by size:
1036 1015 664
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1099 2431 2715
Size: 1099 1332 284
Fragments arranged by size:
1332 1099 284
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 940 1009 2570 2715
Size: 928 12 69 1561 145
Fragments arranged by size:
1561 928 145 69 12
Bpu10I CC'TnA_GC
Cuts at: 0 236 1433 2715
Size: 236 1197 1282
Fragments arranged by size:
1282 1197 236
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 758 2715
Size: 656 102 1957
Fragments arranged by size:
1957 656 102
BsaBI GATnn'nnATC
Cuts at: 0 310 2715
Size: 310 2405
BsaHI Gr'CG_yC
Cuts at: 0 1448 2715
Size: 1448 1267
BsaWI w'CCGG_w
Cuts at: 0 56 1610 2715
Size: 56 1554 1105
Fragments arranged by size:
1554 1105 56
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1807 1837 2715
Size: 1807 30 878
Fragments arranged by size:
1807 878 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 309 925 948 1828 2047 2423 2715
Size: 309 616 23 880 219 376 292
Fragments arranged by size:
880 616 376 309 292 219 23
BseYI C'CCAG_C
Cuts at: 0 1687 2715
Size: 1687 1028
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 948 2416 2715
Size: 948 1468 299
Fragments arranged by size:
1468 948 299
BsiEI CG_ry'CG
Cuts at: 0 901 978 2715
Size: 901 77 1737
Fragments arranged by size:
1737 901 77
BsiHKAI G_wGCw'C
Cuts at: 0 2417 2715
Size: 2417 298
BsmI GAATG_Cn'
Cuts at: 0 651 2481 2715
Size: 651 1830 234
Fragments arranged by size:
1830 651 234
BsmBI CGTCTCn'nnnn_
Cuts at: 0 295 965 2715
Size: 295 670 1750
Fragments arranged by size:
1750 670 295
Bsp1286I G_dGCh'C
Cuts at: 0 636 2417 2715
Size: 636 1781 298
Fragments arranged by size:
1781 636 298
BspEI T'CCGG_A
Cuts at: 0 56 2715
Size: 56 2659
BspHI T'CATG_A
Cuts at: 0 437 2162 2715
Size: 437 1725 553
Fragments arranged by size:
1725 553 437
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1040 1464 2046 2242 2715
Size: 1040 424 582 196 473
Fragments arranged by size:
1040 582 473 424 196
BsrBI CCG'CTC
Cuts at: 0 1223 1396 2715
Size: 1223 173 1319
Fragments arranged by size:
1319 1223 173
BsrDI GCAATG_nn'
Cuts at: 0 1088 1985 2130 2708 2715
Size: 1088 897 145 578 7
Fragments arranged by size:
1088 897 578 145 7
BsrFI r'CCGG_y
Cuts at: 0 754 977 2715
Size: 754 223 1738
Fragments arranged by size:
1738 754 223
BsrGI T'GTAC_A
Cuts at: 0 659 2676 2715
Size: 659 2017 39
Fragments arranged by size:
2017 659 39
BssSI C'ACGA_G
Cuts at: 0 2276 2636 2715
Size: 2276 360 79
Fragments arranged by size:
2276 360 79
BstXI CCAn_nnnn'nTGG
Cuts at: 0 48 1281 2715
Size: 48 1233 1434
Fragments arranged by size:
1434 1233 48
BstYI r'GATC_y
Cuts at: 0 249 594 1077 1122 2715
Size: 249 345 483 45 1593
Fragments arranged by size:
1593 483 345 249 45
BtgI C'CryG_G
Cuts at: 0 365 395 1496 2715
Size: 365 30 1101 1219
Fragments arranged by size:
1219 1101 365 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 735 1607 2429 2715
Size: 735 872 822 286
Fragments arranged by size:
872 822 735 286
BtsI GCAGTG_nn'
Cuts at: 0 1154 2715
Size: 1154 1561
ClaI AT'CG_AT
Cuts at: 0 1829 2715
Size: 1829 886
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1344 1379 2715
Size: 1344 35 1336
Fragments arranged by size:
1344 1336 35
DraI TTT'AAA
Cuts at: 0 528 2715
Size: 528 2187
EaeI y'GGCC_r
Cuts at: 0 898 950 1414 2102 2141 2715
Size: 898 52 464 688 39 574
Fragments arranged by size:
898 688 574 464 52 39
EagI C'GGCC_G
Cuts at: 0 898 2715
Size: 898 1817
EarI CTCTTCn'nnn_
Cuts at: 0 254 464 2602 2715
Size: 254 210 2138 113
Fragments arranged by size:
2138 254 210 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 436 673 1012 2715
Size: 436 237 339 1703
Fragments arranged by size:
1703 436 339 237
EcoICRI GAG'CTC
Cuts at: 0 2415 2715
Size: 2415 300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 658 928 940 1009 2570 2642 2715
Size: 610 48 270 12 69 1561 72 73
Fragments arranged by size:
1561 610 270 73 72 69 48 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 217 1585 2715
Size: 217 1368 1130
Fragments arranged by size:
1368 1130 217
EcoO109I rG'GnC_Cy
Cuts at: 0 36 474 1029 1892 2715
Size: 36 438 555 863 823
Fragments arranged by size:
863 823 555 438 36
EcoRI G'AATT_C
Cuts at: 0 2626 2692 2715
Size: 2626 66 23
Fragments arranged by size:
2626 66 23
EcoRV GAT'ATC
Cuts at: 0 2130 2715
Size: 2130 585
FspI TGC'GCA
Cuts at: 0 289 2715
Size: 289 2426
HaeII r_GCGC'y
Cuts at: 0 946 1024 2715
Size: 946 78 1691
Fragments arranged by size:
1691 946 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 234 266 328 360 1147 1179 1495 1513
Size: 234 32 62 32 787 32 316 18
Cuts at: 1513 1527 1545 2055 2087 2568 2600 2715
Size: 14 18 510 32 481 32 115
Fragments arranged by size:
787 510 481 316 234 115 62 32
32 32 32 32 18 18 14
HincII GTy'rAC
Cuts at: 0 117 1487 2553 2715
Size: 117 1370 1066 162
Fragments arranged by size:
1370 1066 162 117
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 1340 1691 2715
Size: 62 1278 351 1024
Fragments arranged by size:
1278 1024 351 62
MscI TGG'CCA
Cuts at: 0 2104 2143 2715
Size: 2104 39 572
Fragments arranged by size:
2104 572 39
MslI CAynn'nnrTG
Cuts at: 0 692 1513 2715
Size: 692 821 1202
Fragments arranged by size:
1202 821 692
MspA1I CmG'CkG
Cuts at: 0 397 935 2120 2548 2715
Size: 397 538 1185 428 167
Fragments arranged by size:
1185 538 428 397 167
NaeI GCC'GGC
Cuts at: 0 756 2715
Size: 756 1959
NcoI C'CATG_G
Cuts at: 0 365 2715
Size: 365 2350
NgoMIV G'CCGG_C
Cuts at: 0 754 2715
Size: 754 1961
NspI r_CATG'y
Cuts at: 0 1273 2312 2715
Size: 1273 1039 403
Fragments arranged by size:
1273 1039 403
PfoI T'CCnGG_A
Cuts at: 0 212 740 863 1025 1175 2715
Size: 212 528 123 162 150 1540
Fragments arranged by size:
1540 528 212 162 150 123
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 877 909 1807 1839 2715
Size: 877 32 898 32 876
Fragments arranged by size:
898 877 876 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 36 1029 1892 2715
Size: 36 993 863 823
Fragments arranged by size:
993 863 823 36
PspXI vC'TCGA_Gb
Cuts at: 0 1643 2715
Size: 1643 1072
PstI C_TGCA'G
Cuts at: 0 1620 2377 2715
Size: 1620 757 338
Fragments arranged by size:
1620 757 338
PvuII CAG'CTG
Cuts at: 0 2548 2715
Size: 2548 167
SacI G_AGCT'C
Cuts at: 0 2417 2715
Size: 2417 298
SacII CC_GC'GG
Cuts at: 0 398 2715
Size: 398 2317
SanDI GG'GwC_CC
Cuts at: 0 36 2715
Size: 36 2679
SfcI C'TryA_G
Cuts at: 0 188 275 1616 2373 2715
Size: 188 87 1341 757 342
Fragments arranged by size:
1341 757 342 188 87
SmlI C'TyrA_G
Cuts at: 0 671 773 1643 2715
Size: 671 102 870 1072
Fragments arranged by size:
1072 870 671 102
SphI G_CATG'C
Cuts at: 0 1273 2715
Size: 1273 1442
StyI C'CwwG_G
Cuts at: 0 365 800 2715
Size: 365 435 1915
Fragments arranged by size:
1915 435 365
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 704 1372 2715
Size: 704 668 1343
Fragments arranged by size:
1343 704 668
TatI w'GTAC_w
Cuts at: 0 174 659 1043 2136 2676 2715
Size: 174 485 384 1093 540 39
Fragments arranged by size:
1093 540 485 384 174 39
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2533 2715
Size: 2533 182
XhoI C'TCGA_G
Cuts at: 0 1643 2715
Size: 1643 1072
XmnI GAAnn'nnTTC
Cuts at: 0 1822 2715
Size: 1822 893
ZraI GAC'GTC
Cuts at: 0 1449 2715
Size: 1449 1266
Enzymes that do cut:
AarI AatII AclI AcuI AfeI AleI AlfI AloI
AlwNI ApoI AseI AvaI BamHI BanI BanII BbsI
BcgI BclI BglI BglII BlpI BplI BpmI Bpu10I
BpuEI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PfoI PpiI PpuMI PspXI PstI
PvuII SacI SacII SanDI SfcI SmlI SphI StyI
TaqII TatI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AflII AflIII AgeI AhdI ApaI ApaLI
AscI AsiSI AvrII BaeI BbeI BbvCI BciVI BfrBI
Bme1580I BmgBI BmrI BmtI BsaI BsaAI BsiWI BssHII
BstAPI BstBI BstEII BstZ17I Bsu36I DraIII DrdI FalI
FseI FspAI HindIII HpaI KasI KpnI MfeI MluI
NarI NdeI NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PshAI PsiI PspOMI PsrI
PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI TstI Tth111I XbaI XmaI