Restriction map for
pFN21AE2118
(Linear) (Six-base) MAPSORT of: KIEE2118.seq Check: 2924 from: 1 to: 2577
KIEE2118.orf
With 182 enzymes: SgfI *
March 22, 2012 15:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 692 2577
Size: 692 1885
AccI GT'mk_AC
Cuts at: 0 55 872 2177 2577
Size: 55 817 1305 400
Fragments arranged by size:
1305 817 400 55
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 1435 2235 2339 2396 2577
Size: 523 912 800 104 57 181
Fragments arranged by size:
912 800 523 181 104 57
AflIII A'CryG_T
Cuts at: 0 2369 2577
Size: 2369 208
AhdI GACnn_n'nnGTC
Cuts at: 0 879 1839 2577
Size: 879 960 738
Fragments arranged by size:
960 879 738
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 15 47 2577
Size: 15 32 2530
Fragments arranged by size:
2530 32 15
AlwNI CAG_nnn'CTG
Cuts at: 0 1080 1494 1948 2223 2577
Size: 1080 414 454 275 354
Fragments arranged by size:
1080 454 414 354 275
ApaI G_GGCC'C
Cuts at: 0 172 405 1600 2485 2577
Size: 172 233 1195 885 92
Fragments arranged by size:
1195 885 233 172 92
ApaLI G'TGCA_C
Cuts at: 0 537 2577
Size: 537 2040
AvaI C'yCGr_G
Cuts at: 0 112 1430 1859 1913 2577
Size: 112 1318 429 54 664
Fragments arranged by size:
1318 664 429 112 54
AvrII C'CTAG_G
Cuts at: 0 1737 2577
Size: 1737 840
BamHI G'GATC_C
Cuts at: 0 1890 2577
Size: 1890 687
BanI G'GyrC_C
Cuts at: 0 1400 2577
Size: 1400 1177
BanII G_rGCy'C
Cuts at: 0 172 397 405 1256 1600 1942 2386 2485
Size: 172 225 8 851 344 342 444 99
Cuts at: 2485 2577
Size: 92
Fragments arranged by size:
851 444 344 342 225 172 99 92
8
BbsI GAAGACnn'nnnn_
Cuts at: 0 350 408 1811 2577
Size: 350 58 1403 766
Fragments arranged by size:
1403 766 350 58
BbvCI CC'TCA_GC
Cuts at: 0 945 1101 1752 2577
Size: 945 156 651 825
Fragments arranged by size:
945 825 651 156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 221 255 2577
Size: 221 34 2322
Fragments arranged by size:
2322 221 34
BglI GCCn_nnn'nGGC
Cuts at: 0 248 275 829 1733 1762 2577
Size: 248 27 554 904 29 815
Fragments arranged by size:
904 815 554 248 29 27
BglII A'GATC_T
Cuts at: 0 752 2577
Size: 752 1825
BlpI GC'TnA_GC
Cuts at: 0 1941 2577
Size: 1941 636
Bme1580I G_kGCm'C
Cuts at: 0 172 405 541 995 1405 1600 2485 2577
Size: 172 233 136 454 410 195 885 92
Fragments arranged by size:
885 454 410 233 195 172 136 92
BmrI ACTGGGnnnn_n'
Cuts at: 0 2300 2577
Size: 2300 277
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2231 2577
Size: 2231 346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 981 1318 1733 1985 2577
Size: 981 337 415 252 592
Fragments arranged by size:
981 592 415 337 252
Bpu10I CC'TnA_GC
Cuts at: 0 396 945 1101 1372 1752 2577
Size: 396 549 156 271 380 825
Fragments arranged by size:
825 549 396 380 271 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 694 1339 1510 2577
Size: 283 411 645 171 1067
Fragments arranged by size:
1067 645 411 283 171
BsaI GGTCTCn'nnnn_
Cuts at: 0 10 458 2577
Size: 10 448 2119
Fragments arranged by size:
2119 448 10
BsaHI Gr'CG_yC
Cuts at: 0 127 2577
Size: 127 2450
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 15 45 531 561 1608 1638 2359 2389
Size: 15 30 486 30 1047 30 721 30
Cuts at: 2389 2577
Size: 188
Fragments arranged by size:
1047 721 486 188 30 30 30 30
15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 454 1254 1257 1264 1988 2176 2577
Size: 454 800 3 7 724 188 401
Fragments arranged by size:
800 724 454 401 188 7 3
BseYI C'CCAG_C
Cuts at: 0 184 1885 2077 2084 2097 2437 2559 2577
Size: 184 1701 192 7 13 340 122 18
Fragments arranged by size:
1701 340 192 184 122 18 13 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 664 670 1159 1195 2087 2577
Size: 90 574 6 489 36 892 490
Fragments arranged by size:
892 574 490 489 90 36 6
BsiHKAI G_wGCw'C
Cuts at: 0 329 541 796 1256 1283 2377 2577
Size: 329 212 255 460 27 1094 200
Fragments arranged by size:
1094 460 329 255 212 200 27
Bsp1286I G_dGCh'C
Cuts at: 0 172 329 397 405 541 796 995 1256
Size: 172 157 68 8 136 255 199 261
Cuts at: 1256 1283 1405 1600 1942 2377 2386 2485 2577
Size: 27 122 195 342 435 9 99 92
Fragments arranged by size:
435 342 261 255 199 195 172 157
136 122 99 92 68 27 9 8
BspHI T'CATG_A
Cuts at: 0 1794 2577
Size: 1794 783
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 389 686 692 1682 1844 2577
Size: 389 297 6 990 162 733
Fragments arranged by size:
990 733 389 297 162 6
BsrBI CCG'CTC
Cuts at: 0 1395 2346 2358 2478 2577
Size: 1395 951 12 120 99
Fragments arranged by size:
1395 951 120 99 12
BsrDI GCAATG_nn'
Cuts at: 0 855 1094 2577
Size: 855 239 1483
Fragments arranged by size:
1483 855 239
BsrGI T'GTAC_A
Cuts at: 0 2111 2577
Size: 2111 466
BstEII G'GTnAC_C
Cuts at: 0 188 2577
Size: 188 2389
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1711 2577
Size: 1711 866
BstYI r'GATC_y
Cuts at: 0 705 752 920 1019 1076 1890 2577
Size: 705 47 168 99 57 814 687
Fragments arranged by size:
814 705 687 168 99 57 47
Bsu36I CC'TnA_GG
Cuts at: 0 836 1592 2055 2577
Size: 836 756 463 522
Fragments arranged by size:
836 756 522 463
BtgI C'CryG_G
Cuts at: 0 432 650 1623 1782 2577
Size: 432 218 973 159 795
Fragments arranged by size:
973 795 432 218 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 642 2577
Size: 642 1935
BtsI GCAGTG_nn'
Cuts at: 0 244 258 391 2104 2577
Size: 244 14 133 1713 473
Fragments arranged by size:
1713 473 244 133 14
DraIII CAC_nnn'GTG
Cuts at: 0 1933 2577
Size: 1933 644
EaeI y'GGCC_r
Cuts at: 0 612 2347 2577
Size: 612 1735 230
Fragments arranged by size:
1735 612 230
EarI CTCTTCn'nnn_
Cuts at: 0 1217 1468 2153 2577
Size: 1217 251 685 424
Fragments arranged by size:
1217 685 424 251
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1895 2577
Size: 1895 682
EcoICRI GAG'CTC
Cuts at: 0 1254 2577
Size: 1254 1323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 981 1318 1435 1733 1985 2235 2339
Size: 523 458 337 117 298 252 250 104
Cuts at: 2339 2396 2577
Size: 57 181
Fragments arranged by size:
523 458 337 298 252 250 181 117
104 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 64 352 1222 2003 2015 2577
Size: 64 288 870 781 12 562
Fragments arranged by size:
870 781 562 288 64 12
EcoO109I rG'GnC_Cy
Cuts at: 0 120 168 267 357 401 1202 1434 1596
Size: 120 48 99 90 44 801 232 162
Cuts at: 1596 1597 1671 1734 1747 1856 2008 2051 2081
Size: 1 74 63 13 109 152 43 30
Cuts at: 2081 2481 2482 2577
Size: 400 1 95
Fragments arranged by size:
801 400 232 162 152 120 109 99
95 90 74 63 48 44 43 30
13 1 1
EcoRV GAT'ATC
Cuts at: 0 1521 2577
Size: 1521 1056
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 822 2577
Size: 822 1755
FspI TGC'GCA
Cuts at: 0 79 2577
Size: 79 2498
HaeII r_GCGC'y
Cuts at: 0 429 1655 2577
Size: 429 1226 922
Fragments arranged by size:
1226 922 429
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 697 729 865 897 1084 1099 1116 1131
Size: 697 32 136 32 187 15 17 15
Cuts at: 1131 1825 1857 1882 1914 2182 2214 2251 2283
Size: 694 32 25 32 268 32 37 32
Cuts at: 2283 2344 2359 2376 2391 2577
Size: 61 15 17 15 186
Fragments arranged by size:
697 694 268 187 186 136 61 37
32 32 32 32 32 32 25 17
17 15 15 15 15
HincII GTy'rAC
Cuts at: 0 642 873 2577
Size: 642 231 1704
Fragments arranged by size:
1704 642 231
HindIII A'AGCT_T
Cuts at: 0 1385 1462 2577
Size: 1385 77 1115
Fragments arranged by size:
1385 1115 77
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1095 2558 2577
Size: 1095 1463 19
Fragments arranged by size:
1463 1095 19
MslI CAynn'nnrTG
Cuts at: 0 565 799 2577
Size: 565 234 1778
Fragments arranged by size:
1778 565 234
MspA1I CmG'CkG
Cuts at: 0 184 591 1105 1347 1693 2101 2577
Size: 184 407 514 242 346 408 476
Fragments arranged by size:
514 476 408 407 346 242 184
NcoI C'CATG_G
Cuts at: 0 650 2577
Size: 650 1927
NspI r_CATG'y
Cuts at: 0 726 2232 2373 2577
Size: 726 1506 141 204
Fragments arranged by size:
1506 726 204 141
PasI CC'CwG_GG
Cuts at: 0 172 405 582 1729 2379 2577
Size: 172 233 177 1147 650 198
Fragments arranged by size:
1147 650 233 198 177 172
PciI A'CATG_T
Cuts at: 0 2369 2577
Size: 2369 208
PflMI CCAn_nnn'nTGG
Cuts at: 0 956 2272 2577
Size: 956 1316 305
Fragments arranged by size:
1316 956 305
PfoI T'CCnGG_A
Cuts at: 0 374 2577
Size: 374 2203
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 15 47 2577
Size: 15 32 2530
Fragments arranged by size:
2530 32 15
PpuMI rG'GwC_Cy
Cuts at: 0 120 357 1202 1671 1747 1856 2051 2081
Size: 120 237 845 469 76 109 195 30
Cuts at: 2081 2577
Size: 496
Fragments arranged by size:
845 496 469 237 195 120 109 76
30
PspOMI G'GGCC_C
Cuts at: 0 168 401 1596 2481 2577
Size: 168 233 1195 885 96
Fragments arranged by size:
1195 885 233 168 96
PstI C_TGCA'G
Cuts at: 0 146 239 386 2577
Size: 146 93 147 2191
Fragments arranged by size:
2191 147 146 93
PvuII CAG'CTG
Cuts at: 0 184 591 1105 1347 1693 2101 2577
Size: 184 407 514 242 346 408 476
Fragments arranged by size:
514 476 408 407 346 242 184
SacI G_AGCT'C
Cuts at: 0 1256 2577
Size: 1256 1321
SalI G'TCGA_C
Cuts at: 0 871 2577
Size: 871 1706
SanDI GG'GwC_CC
Cuts at: 0 1747 1856 2081 2577
Size: 1747 109 225 496
Fragments arranged by size:
1747 496 225 109
SfcI C'TryA_G
Cuts at: 0 142 235 382 2577
Size: 142 93 147 2195
Fragments arranged by size:
2195 147 142 93
SmaI CCC'GGG
Cuts at: 0 1432 1861 1915 2577
Size: 1432 429 54 662
Fragments arranged by size:
1432 662 429 54
SmlI C'TyrA_G
Cuts at: 0 262 709 1354 1525 2577
Size: 262 447 645 171 1052
Fragments arranged by size:
1052 645 447 262 171
StuI AGG'CCT
Cuts at: 0 1409 1765 2577
Size: 1409 356 812
Fragments arranged by size:
1409 812 356
StyI C'CwwG_G
Cuts at: 0 650 773 1655 1737 2577
Size: 650 123 882 82 840
Fragments arranged by size:
882 840 650 123 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 378 556 732 1574 2577
Size: 378 178 176 842 1003
Fragments arranged by size:
1003 842 378 178 176
TatI w'GTAC_w
Cuts at: 0 1110 2111 2577
Size: 1110 1001 466
Fragments arranged by size:
1110 1001 466
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 370 402 531 563 1608 1640 2577
Size: 370 32 129 32 1045 32 937
Fragments arranged by size:
1045 937 370 129 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1533 1616 2577
Size: 1533 83 961
Fragments arranged by size:
1533 961 83
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 81 250 2577
Size: 81 169 2327
Fragments arranged by size:
2327 169 81
XmaI C'CCGG_G
Cuts at: 0 1430 1859 1913 2577
Size: 1430 429 54 664
Fragments arranged by size:
1430 664 429 54
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AloI AlwNI ApaI
ApaLI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI
FspI HaeII Hin4I HincII HindIII MmeI MslI MspA1I
NcoI NspI PasI PciI PflMI PfoI PpiI PpuMI
PspOMI PstI PvuII SacI SalI SanDI SfcI SmaI
SmlI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AlfI
ApoI AscI AseI AsiSI BaeI BbeI BciVI BclI
BfrBI BmgBI BmtI BsaAI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BsrFI BssHII BssSI BstAPI BstBI
BstZ17I ClaI CspCI DraI DrdI EagI EcoRI FseI
FspAI HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XhoI XmnI ZraI