Restriction map for
pFN21AE2116
(Linear) (Six-base) MAPSORT of: KIEE2116.seq Check: 9235 from: 1 to: 2625
KIEE2116.orf
With 182 enzymes: SgfI *
July 3, 2012 17:09 ..
AccI GT'mk_AC
Cuts at: 0 725 827 1881 1910 2625
Size: 725 102 1054 29 715
Fragments arranged by size:
1054 725 715 102 29
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1210 2625
Size: 1210 1415
AflIII A'CryG_T
Cuts at: 0 1435 2625
Size: 1435 1190
ApoI r'AATT_y
Cuts at: 0 404 852 1063 1420 1467 1493 1672 1717
Size: 404 448 211 357 47 26 179 45
Cuts at: 1717 2105 2134 2625
Size: 388 29 491
Fragments arranged by size:
491 448 404 388 357 211 179 47
45 29 26
AseI AT'TA_AT
Cuts at: 0 233 1349 2625
Size: 233 1116 1276
Fragments arranged by size:
1276 1116 233
AvaI C'yCGr_G
Cuts at: 0 217 833 1268 2625
Size: 217 616 435 1357
Fragments arranged by size:
1357 616 435 217
BanI G'GyrC_C
Cuts at: 0 304 2625
Size: 304 2321
BanII G_rGCy'C
Cuts at: 0 1199 2625
Size: 1199 1426
BbsI GAAGACnn'nnnn_
Cuts at: 0 563 620 2625
Size: 563 57 2005
Fragments arranged by size:
2005 563 57
BbvCI CC'TCA_GC
Cuts at: 0 479 2625
Size: 479 2146
BclI T'GATC_A
Cuts at: 0 971 2030 2625
Size: 971 1059 595
Fragments arranged by size:
1059 971 595
BglII A'GATC_T
Cuts at: 0 2055 2625
Size: 2055 570
BlpI GC'TnA_GC
Cuts at: 0 279 2625
Size: 279 2346
Bme1580I G_kGCm'C
Cuts at: 0 309 2434 2625
Size: 309 2125 191
Fragments arranged by size:
2125 309 191
BmgBI CAC'GTC
Cuts at: 0 1585 2625
Size: 1585 1040
BmrI ACTGGGnnnn_n'
Cuts at: 0 1864 1960 2625
Size: 1864 96 665
Fragments arranged by size:
1864 665 96
BmtI G_CTAG'C
Cuts at: 0 65 2625
Size: 65 2560
Bpu10I CC'TnA_GC
Cuts at: 0 479 2625
Size: 479 2146
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 618 2541 2625
Size: 310 308 1923 84
Fragments arranged by size:
1923 310 308 84
BsaAI yAC'GTr
Cuts at: 0 2600 2625
Size: 2600 25
BsaBI GATnn'nnATC
Cuts at: 0 254 2625
Size: 254 2371
BsaWI w'CCGG_w
Cuts at: 0 500 2547 2625
Size: 500 2047 78
Fragments arranged by size:
2047 500 78
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1145 1175 2625
Size: 1145 30 1450
Fragments arranged by size:
1450 1145 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 2625
Size: 129 2496
BsiHKAI G_wGCw'C
Cuts at: 0 2445 2625
Size: 2445 180
BsmI GAATG_Cn'
Cuts at: 0 1458 2625
Size: 1458 1167
Bsp1286I G_dGCh'C
Cuts at: 0 309 1199 2434 2445 2625
Size: 309 890 1235 11 180
Fragments arranged by size:
1235 890 309 180 11
BspEI T'CCGG_A
Cuts at: 0 500 2547 2625
Size: 500 2047 78
Fragments arranged by size:
2047 500 78
BsrDI GCAATG_nn'
Cuts at: 0 1065 2625
Size: 1065 1560
BsrGI T'GTAC_A
Cuts at: 0 1502 1890 2625
Size: 1502 388 735
Fragments arranged by size:
1502 735 388
BstAPI GCAn_nnn'nTGC
Cuts at: 0 595 2625
Size: 595 2030
BstBI TT'CG_AA
Cuts at: 0 1114 2625
Size: 1114 1511
BstEII G'GTnAC_C
Cuts at: 0 2065 2217 2507 2625
Size: 2065 152 290 118
Fragments arranged by size:
2065 290 152 118
BstYI r'GATC_y
Cuts at: 0 837 2055 2625
Size: 837 1218 570
Fragments arranged by size:
1218 837 570
BstZ17I GTA'TAC
Cuts at: 0 828 1882 2625
Size: 828 1054 743
Fragments arranged by size:
1054 828 743
BtgI C'CryG_G
Cuts at: 0 1901 2625
Size: 1901 724
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 949 2625
Size: 949 1676
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1130 1165 2625
Size: 1130 35 1460
Fragments arranged by size:
1460 1130 35
DraI TTT'AAA
Cuts at: 0 366 1985 2625
Size: 366 1619 640
Fragments arranged by size:
1619 640 366
EarI CTCTTCn'nnn_
Cuts at: 0 383 456 545 1187 1334 2416 2625
Size: 383 73 89 642 147 1082 209
Fragments arranged by size:
1082 642 383 209 147 89 73
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1210 2625
Size: 1210 1415
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2450 2625
Size: 2450 175
EcoO109I rG'GnC_Cy
Cuts at: 0 682 1264 2625
Size: 682 582 1361
Fragments arranged by size:
1361 682 582
EcoRI G'AATT_C
Cuts at: 0 1493 2625
Size: 1493 1132
EcoRV GAT'ATC
Cuts at: 0 2045 2625
Size: 2045 580
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 299 2587 2625
Size: 299 2288 38
Fragments arranged by size:
2288 299 38
HaeII r_GCGC'y
Cuts at: 0 353 2625
Size: 353 2272
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 679 711 2067 2099 2625
Size: 679 32 1356 32 526
Fragments arranged by size:
1356 679 526 32 32
HincII GTy'rAC
Cuts at: 0 119 2625
Size: 119 2506
HpaI GTT'AAC
Cuts at: 0 119 2625
Size: 119 2506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 2625
Size: 196 2429
MspA1I CmG'CkG
Cuts at: 0 82 1728 2625
Size: 82 1646 897
Fragments arranged by size:
1646 897 82
NcoI C'CATG_G
Cuts at: 0 1901 2625
Size: 1901 724
NdeI CA'TA_TG
Cuts at: 0 2213 2625
Size: 2213 412
NheI G'CTAG_C
Cuts at: 0 61 2625
Size: 61 2564
NspI r_CATG'y
Cuts at: 0 593 1439 1503 2622 2625
Size: 593 846 64 1119 3
Fragments arranged by size:
1119 846 593 64 3
PciI A'CATG_T
Cuts at: 0 1435 2625
Size: 1435 1190
PflMI CCAn_nnn'nTGG
Cuts at: 0 1759 2625
Size: 1759 866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1408 1440 2625
Size: 1408 32 1185
Fragments arranged by size:
1408 1185 32
PpuMI rG'GwC_Cy
Cuts at: 0 1264 2625
Size: 1264 1361
PshAI GACnn'nnGTC
Cuts at: 0 981 2625
Size: 981 1644
PsiI TTA'TAA
Cuts at: 0 227 1046 2625
Size: 227 819 1579
Fragments arranged by size:
1579 819 227
PspXI vC'TCGA_Gb
Cuts at: 0 217 1268 2625
Size: 217 1051 1357
Fragments arranged by size:
1357 1051 217
PstI C_TGCA'G
Cuts at: 0 1647 2625
Size: 1647 978
PvuII CAG'CTG
Cuts at: 0 82 1728 2625
Size: 82 1646 897
Fragments arranged by size:
1646 897 82
SapI GCTCTTCn'nnn_
Cuts at: 0 1187 2625
Size: 1187 1438
ScaI AGT'ACT
Cuts at: 0 1853 2242 2625
Size: 1853 389 383
Fragments arranged by size:
1853 389 383
SfcI C'TryA_G
Cuts at: 0 1530 1643 1911 2318 2625
Size: 1530 113 268 407 307
Fragments arranged by size:
1530 407 307 268 113
SmlI C'TyrA_G
Cuts at: 0 217 289 633 833 1268 2556 2625
Size: 217 72 344 200 435 1288 69
Fragments arranged by size:
1288 435 344 217 200 72 69
SphI G_CATG'C
Cuts at: 0 2622 2625
Size: 2622 3
StyI C'CwwG_G
Cuts at: 0 521 960 1901 2625
Size: 521 439 941 724
Fragments arranged by size:
941 724 521 439
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1979 2625
Size: 1979 646
TatI w'GTAC_w
Cuts at: 0 1502 1851 1890 2240 2625
Size: 1502 349 39 350 385
Fragments arranged by size:
1502 385 350 349 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1145 1177 2625
Size: 1145 32 1448
Fragments arranged by size:
1448 1145 32
XbaI T'CTAG_A
Cuts at: 0 1371 2292 2625
Size: 1371 921 333
Fragments arranged by size:
1371 921 333
XhoI C'TCGA_G
Cuts at: 0 217 833 1268 2625
Size: 217 616 435 1357
Fragments arranged by size:
1357 616 435 217
XmnI GAAnn'nnTTC
Cuts at: 0 856 1420 1721 2378 2625
Size: 856 564 301 657 247
Fragments arranged by size:
856 657 564 301 247
Enzymes that do cut:
AccI AcuI AflIII ApoI AseI AvaI BanI BanII
BbsI BbvCI BclI BglII BlpI Bme1580I BmgBI BmrI
BmtI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BsgI
BsiHKAI BsmI Bsp1286I BspEI BsrDI BsrGI BstAPI BstBI
BstEII BstYI BstZ17I BtgI BtgZI CspCI DraI EarI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII HpaI MmeI MspA1I NcoI NdeI NheI NspI
PciI PflMI PpiI PpuMI PshAI PsiI PspXI PstI
PvuII SapI ScaI SfcI SmlI SphI StyI TaqII
TatI TstI XbaI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BbeI BcgI BciVI BfrBI BglI
BplI BpmI BsaI BsaHI BseRI BseYI BsiEI BsiWI
BsmBI BspHI BspMI BsrBI BsrFI BssHII BssSI BstXI
Bsu36I BtsI ClaI DraIII DrdI EaeI EagI EciI
EcoICRI FseI FspI FspAI HindIII KasI KpnI MfeI
MluI MscI MslI NaeI NarI NgoMIV NotI NruI
NsiI PacI PasI PfoI PmeI PmlI PspOMI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI Tth111I XcmI XmaI ZraI