Restriction map for
pFN21AE2115
(Linear) (Six-base) MAPSORT of: KIEE2115.seq Check: 7430 from: 1 to: 912
KIEE2115.orf
With 182 enzymes: SgfI *
July 15, 2010 18:55 ..
AflIII A'CryG_T
Cuts at: 0 467 732 912
Size: 467 265 180
Fragments arranged by size:
467 265 180
AgeI A'CCGG_T
Cuts at: 0 712 912
Size: 712 200
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 494 526 912
Size: 494 32 386
Fragments arranged by size:
494 386 32
ApaI G_GGCC'C
Cuts at: 0 409 565 912
Size: 409 156 347
Fragments arranged by size:
409 347 156
AvaI C'yCGr_G
Cuts at: 0 633 912
Size: 633 279
BanII G_rGCy'C
Cuts at: 0 409 460 565 912
Size: 409 51 105 347
Fragments arranged by size:
409 347 105 51
BclI T'GATC_A
Cuts at: 0 362 912
Size: 362 550
Bme1580I G_kGCm'C
Cuts at: 0 409 565 912
Size: 409 156 347
Fragments arranged by size:
409 347 156
BmrI ACTGGGnnnn_n'
Cuts at: 0 335 912
Size: 335 577
BsaWI w'CCGG_w
Cuts at: 0 712 912
Size: 712 200
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 416 446 494 524 912
Size: 416 30 48 30 388
Fragments arranged by size:
416 388 48 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 769 912
Size: 769 143
BseYI C'CCAG_C
Cuts at: 0 398 912
Size: 398 514
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 912
Size: 163 749
BsiHKAI G_wGCw'C
Cuts at: 0 170 227 460 808 912
Size: 170 57 233 348 104
Fragments arranged by size:
348 233 170 104 57
Bsp1286I G_dGCh'C
Cuts at: 0 170 227 409 460 565 808 912
Size: 170 57 182 51 105 243 104
Fragments arranged by size:
243 182 170 105 104 57 51
BsrDI GCAATG_nn'
Cuts at: 0 148 912
Size: 148 764
BsrFI r'CCGG_y
Cuts at: 0 712 912
Size: 712 200
BstEII G'GTnAC_C
Cuts at: 0 106 715 912
Size: 106 609 197
Fragments arranged by size:
609 197 106
BtgI C'CryG_G
Cuts at: 0 410 573 912
Size: 410 163 339
Fragments arranged by size:
410 339 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 781 912
Size: 781 131
BtsI GCAGTG_nn'
Cuts at: 0 524 643 912
Size: 524 119 269
Fragments arranged by size:
524 269 119
DraIII CAC_nnn'GTG
Cuts at: 0 114 912
Size: 114 798
EaeI y'GGCC_r
Cuts at: 0 58 912
Size: 58 854
EarI CTCTTCn'nnn_
Cuts at: 0 119 787 912
Size: 119 668 125
Fragments arranged by size:
668 125 119
EcoICRI GAG'CTC
Cuts at: 0 458 912
Size: 458 454
EcoO109I rG'GnC_Cy
Cuts at: 0 53 406 562 581 746 912
Size: 53 353 156 19 165 166
Fragments arranged by size:
353 166 165 156 53 19
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 267 912
Size: 267 645
FspI TGC'GCA
Cuts at: 0 523 912
Size: 523 389
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 354 386 912
Size: 354 32 526
Fragments arranged by size:
526 354 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 833 912
Size: 131 702 79
Fragments arranged by size:
702 131 79
MscI TGG'CCA
Cuts at: 0 60 912
Size: 60 852
MslI CAynn'nnrTG
Cuts at: 0 19 230 731 912
Size: 19 211 501 181
Fragments arranged by size:
501 211 181 19
NspI r_CATG'y
Cuts at: 0 471 736 912
Size: 471 265 176
Fragments arranged by size:
471 265 176
PciI A'CATG_T
Cuts at: 0 467 732 912
Size: 467 265 180
Fragments arranged by size:
467 265 180
PfoI T'CCnGG_A
Cuts at: 0 451 912
Size: 451 461
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 494 526 912
Size: 494 32 386
Fragments arranged by size:
494 386 32
PpuMI rG'GwC_Cy
Cuts at: 0 581 746 912
Size: 581 165 166
Fragments arranged by size:
581 166 165
PspOMI G'GGCC_C
Cuts at: 0 405 561 912
Size: 405 156 351
Fragments arranged by size:
405 351 156
PspXI vC'TCGA_Gb
Cuts at: 0 633 912
Size: 633 279
PstI C_TGCA'G
Cuts at: 0 50 448 519 849 912
Size: 50 398 71 330 63
Fragments arranged by size:
398 330 71 63 50
SacI G_AGCT'C
Cuts at: 0 460 912
Size: 460 452
SbfI CC_TGCA'GG
Cuts at: 0 849 912
Size: 849 63
SexAI A'CCwGG_T
Cuts at: 0 110 912
Size: 110 802
SfcI C'TryA_G
Cuts at: 0 46 291 444 515 585 845 912
Size: 46 245 153 71 70 260 67
Fragments arranged by size:
260 245 153 71 70 67 46
SmlI C'TyrA_G
Cuts at: 0 633 912
Size: 633 279
StyI C'CwwG_G
Cuts at: 0 242 912
Size: 242 670
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 586 705 912
Size: 586 119 207
Fragments arranged by size:
586 207 119
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 416 448 912
Size: 416 32 464
Fragments arranged by size:
464 416 32
Tth111I GACn'n_nGTC
Cuts at: 0 438 912
Size: 438 474
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 348 912
Size: 348 564
XhoI C'TCGA_G
Cuts at: 0 633 912
Size: 633 279
XmnI GAAnn'nnTTC
Cuts at: 0 84 669 912
Size: 84 585 243
Fragments arranged by size:
585 243 84
Enzymes that do cut:
AflIII AgeI AloI ApaI AvaI BanII BclI Bme1580I
BmrI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BsrDI BsrFI BstEII BtgI BtgZI BtsI DraIII EaeI
EarI EcoICRI EcoO109I FalI FspI Hin4I MmeI MscI
MslI NspI PciI PfoI PpiI PpuMI PspOMI PspXI
PstI SacI SbfI SexAI SfcI SmlI StyI TaqII
TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I ClaI CspCI DraI DrdI EagI EciI Eco57MI
EcoNI EcoRI EcoRV FseI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PshAI PsiI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI XbaI XmaI ZraI