Restriction map for
pFN21AE2111
(Linear) (Six-base) MAPSORT of: KIEE2111.seq Check: 7163 from: 1 to: 2478
KIEE2111.orf
With 182 enzymes: SgfI *
March 22, 2012 15:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1635 2478
Size: 1635 843
AatII G_ACGT'C
Cuts at: 0 2139 2478
Size: 2139 339
AccI GT'mk_AC
Cuts at: 0 849 2478
Size: 849 1629
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 354 433 1258 2478
Size: 337 17 79 825 1220
Fragments arranged by size:
1220 825 337 79 17
AflII C'TTAA_G
Cuts at: 0 604 2478
Size: 604 1874
AflIII A'CryG_T
Cuts at: 0 1599 2478
Size: 1599 879
AhdI GACnn_n'nnGTC
Cuts at: 0 996 2478
Size: 996 1482
AleI CACnn'nnGTG
Cuts at: 0 1852 2287 2478
Size: 1852 435 191
Fragments arranged by size:
1852 435 191
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1081 1870 2478
Size: 1081 789 608
Fragments arranged by size:
1081 789 608
AlwNI CAG_nnn'CTG
Cuts at: 0 76 131 810 975 2478
Size: 76 55 679 165 1503
Fragments arranged by size:
1503 679 165 76 55
ApaI G_GGCC'C
Cuts at: 0 1292 1674 2478
Size: 1292 382 804
Fragments arranged by size:
1292 804 382
AscI GG'CGCG_CC
Cuts at: 0 2128 2478
Size: 2128 350
AvaI C'yCGr_G
Cuts at: 0 108 831 1530 1724 2478
Size: 108 723 699 194 754
Fragments arranged by size:
754 723 699 194 108
BamHI G'GATC_C
Cuts at: 0 883 1025 1040 2478
Size: 883 142 15 1438
Fragments arranged by size:
1438 883 142 15
BanI G'GyrC_C
Cuts at: 0 1095 1128 1331 1522 1854 1861 1934 2478
Size: 1095 33 203 191 332 7 73 544
Fragments arranged by size:
1095 544 332 203 191 73 33 7
BanII G_rGCy'C
Cuts at: 0 386 652 676 1175 1292 1376 1674 2478
Size: 386 266 24 499 117 84 298 804
Fragments arranged by size:
804 499 386 298 266 117 84 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 801 1949 2478
Size: 801 1148 529
Fragments arranged by size:
1148 801 529
BbvCI CC'TCA_GC
Cuts at: 0 1435 2030 2478
Size: 1435 595 448
Fragments arranged by size:
1435 595 448
BciVI GTATCCnnnnn_n'
Cuts at: 0 700 2478
Size: 700 1778
BglI GCCn_nnn'nGGC
Cuts at: 0 1296 1822 2478
Size: 1296 526 656
Fragments arranged by size:
1296 656 526
Bme1580I G_kGCm'C
Cuts at: 0 1100 1292 1493 1538 1674 2223 2478
Size: 1100 192 201 45 136 549 255
Fragments arranged by size:
1100 549 255 201 192 136 45
BmgBI CAC'GTC
Cuts at: 0 1602 1614 2478
Size: 1602 12 864
Fragments arranged by size:
1602 864 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 64 1525 2478
Size: 64 1461 953
Fragments arranged by size:
1461 953 64
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 828 838 1207 1246 1977 2140 2161
Size: 400 428 10 369 39 731 163 21
Cuts at: 2161 2478
Size: 317
Fragments arranged by size:
731 428 400 369 317 163 39 21
10
Bpu10I CC'TnA_GC
Cuts at: 0 1435 2030 2478
Size: 1435 595 448
Fragments arranged by size:
1435 595 448
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2029 2478
Size: 2029 449
BsaI GGTCTCn'nnnn_
Cuts at: 0 843 2478
Size: 843 1635
BsaAI yAC'GTr
Cuts at: 0 1121 1422 1494 1548 2478
Size: 1121 301 72 54 930
Fragments arranged by size:
1121 930 301 72 54
BsaHI Gr'CG_yC
Cuts at: 0 2136 2478
Size: 2136 342
BsaWI w'CCGG_w
Cuts at: 0 2036 2478
Size: 2036 442
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 80 110 162 192 2478
Size: 80 30 52 30 2286
Fragments arranged by size:
2286 80 52 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 415 831 1183 1231 1243 1270 1405
Size: 97 318 416 352 48 12 27 135
Cuts at: 1405 2137 2158 2419 2458 2478
Size: 732 21 261 39 20
Fragments arranged by size:
732 416 352 318 261 135 97 48
39 27 21 20 12
BseYI C'CCAG_C
Cuts at: 0 939 2094 2114 2222 2478
Size: 939 1155 20 108 256
Fragments arranged by size:
1155 939 256 108 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 1099 1216 1570 2068 2478
Size: 345 754 117 354 498 410
Fragments arranged by size:
754 498 410 354 345 117
BsiEI CG_ry'CG
Cuts at: 0 1580 2478
Size: 1580 898
BsiHKAI G_wGCw'C
Cuts at: 0 676 1175 1421 2015 2172 2478
Size: 676 499 246 594 157 306
Fragments arranged by size:
676 594 499 306 246 157
BsmI GAATG_Cn'
Cuts at: 0 267 2478
Size: 267 2211
BsmBI CGTCTCn'nnnn_
Cuts at: 0 770 2478
Size: 770 1708
Bsp1286I G_dGCh'C
Cuts at: 0 386 652 676 1100 1175 1292 1376 1421
Size: 386 266 24 424 75 117 84 45
Cuts at: 1421 1493 1538 1674 2015 2172 2223 2478
Size: 72 45 136 341 157 51 255
Fragments arranged by size:
424 386 341 266 255 157 136 117
84 75 72 51 45 45 24
BspEI T'CCGG_A
Cuts at: 0 2036 2478
Size: 2036 442
BspHI T'CATG_A
Cuts at: 0 515 1983 2478
Size: 515 1468 495
Fragments arranged by size:
1468 515 495
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 719 1071 1635 2478
Size: 719 352 564 843
Fragments arranged by size:
843 719 564 352
BsrBI CCG'CTC
Cuts at: 0 1233 2478
Size: 1233 1245
BsrFI r'CCGG_y
Cuts at: 0 922 1579 1945 2023 2478
Size: 922 657 366 78 455
Fragments arranged by size:
922 657 455 366 78
BssHII G'CGCG_C
Cuts at: 0 2128 2478
Size: 2128 350
BssSI C'ACGA_G
Cuts at: 0 1387 2478
Size: 1387 1091
BstAPI GCAn_nnn'nTGC
Cuts at: 0 273 2478
Size: 273 2205
BstXI CCAn_nnnn'nTGG
Cuts at: 0 676 1459 2478
Size: 676 783 1019
Fragments arranged by size:
1019 783 676
BstYI r'GATC_y
Cuts at: 0 192 883 1025 1040 2478
Size: 192 691 142 15 1438
Fragments arranged by size:
1438 691 192 142 15
Bsu36I CC'TnA_GG
Cuts at: 0 215 2478
Size: 215 2263
BtgI C'CryG_G
Cuts at: 0 1710 1950 2478
Size: 1710 240 528
Fragments arranged by size:
1710 528 240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 998 1456 2478
Size: 998 458 1022
Fragments arranged by size:
1022 998 458
BtsI GCAGTG_nn'
Cuts at: 0 228 262 1906 2478
Size: 228 34 1644 572
Fragments arranged by size:
1644 572 228 34
ClaI AT'CG_AT
Cuts at: 0 530 2478
Size: 530 1948
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 227 262 2478
Size: 227 35 2216
Fragments arranged by size:
2216 227 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2425 2478
Size: 2425 53
EaeI y'GGCC_r
Cuts at: 0 173 1446 1467 1496 1577 1947 2478
Size: 173 1273 21 29 81 370 531
Fragments arranged by size:
1273 531 370 173 81 29 21
EagI C'GGCC_G
Cuts at: 0 1577 2478
Size: 1577 901
EarI CTCTTCn'nnn_
Cuts at: 0 400 1831 2068 2478
Size: 400 1431 237 410
Fragments arranged by size:
1431 410 400 237
EcoICRI GAG'CTC
Cuts at: 0 674 1173 2478
Size: 674 499 1305
Fragments arranged by size:
1305 674 499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 354 400 433 828 838 1207 1246
Size: 337 17 46 33 395 10 369 39
Cuts at: 1246 1258 1977 2140 2161 2478
Size: 12 719 163 21 317
Fragments arranged by size:
719 395 369 337 317 163 46 39
33 21 17 12 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 319 2478
Size: 319 2159
EcoO109I rG'GnC_Cy
Cuts at: 0 1288 1289 1670 1692 1721 2478
Size: 1288 1 381 22 29 757
Fragments arranged by size:
1288 757 381 29 22 1
FseI GG_CCGG'CC
Cuts at: 0 1583 2027 2478
Size: 1583 444 451
Fragments arranged by size:
1583 451 444
FspI TGC'GCA
Cuts at: 0 1249 2478
Size: 1249 1229
FspAI rTGC'GCAy
Cuts at: 0 1249 2478
Size: 1249 1229
HaeII r_GCGC'y
Cuts at: 0 189 1751 1844 2478
Size: 189 1562 93 634
Fragments arranged by size:
1562 634 189 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 162 194 658 690 982 1014 1267 1299
Size: 162 32 464 32 292 32 253 32
Cuts at: 1299 2478
Size: 1179
Fragments arranged by size:
1179 464 292 253 162 32 32 32
32
HincII GTy'rAC
Cuts at: 0 903 2478
Size: 903 1575
MscI TGG'CCA
Cuts at: 0 1469 1498 2478
Size: 1469 29 980
Fragments arranged by size:
1469 980 29
MslI CAynn'nnrTG
Cuts at: 0 1709 1852 2242 2287 2478
Size: 1709 143 390 45 191
Fragments arranged by size:
1709 390 191 143 45
MspA1I CmG'CkG
Cuts at: 0 525 826 1349 2118 2478
Size: 525 301 523 769 360
Fragments arranged by size:
769 525 523 360 301
NaeI GCC'GGC
Cuts at: 0 924 1581 2025 2478
Size: 924 657 444 453
Fragments arranged by size:
924 657 453 444
NcoI C'CATG_G
Cuts at: 0 1710 2478
Size: 1710 768
NgoMIV G'CCGG_C
Cuts at: 0 922 1579 2023 2478
Size: 922 657 444 455
Fragments arranged by size:
922 657 455 444
PasI CC'CwG_GG
Cuts at: 0 1294 2018 2310 2478
Size: 1294 724 292 168
Fragments arranged by size:
1294 724 292 168
PflMI CCAn_nnn'nTGG
Cuts at: 0 76 756 1185 2478
Size: 76 680 429 1293
Fragments arranged by size:
1293 680 429 76
PfoI T'CCnGG_A
Cuts at: 0 136 815 1376 1409 2478
Size: 136 679 561 33 1069
Fragments arranged by size:
1069 679 561 136 33
PmlI CAC'GTG
Cuts at: 0 1494 2478
Size: 1494 984
PpuMI rG'GwC_Cy
Cuts at: 0 1721 2478
Size: 1721 757
PshAI GACnn'nnGTC
Cuts at: 0 2001 2478
Size: 2001 477
PspOMI G'GGCC_C
Cuts at: 0 1288 1670 2478
Size: 1288 382 808
Fragments arranged by size:
1288 808 382
PvuII CAG'CTG
Cuts at: 0 525 826 2118 2478
Size: 525 301 1292 360
Fragments arranged by size:
1292 525 360 301
SacI G_AGCT'C
Cuts at: 0 676 1175 2478
Size: 676 499 1303
Fragments arranged by size:
1303 676 499
SapI GCTCTTCn'nnn_
Cuts at: 0 1831 2478
Size: 1831 647
SfcI C'TryA_G
Cuts at: 0 123 660 2349 2478
Size: 123 537 1689 129
Fragments arranged by size:
1689 537 129 123
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1296 2478
Size: 1296 1182
SmlI C'TyrA_G
Cuts at: 0 604 2008 2478
Size: 604 1404 470
Fragments arranged by size:
1404 604 470
StyI C'CwwG_G
Cuts at: 0 26 1637 1710 2478
Size: 26 1611 73 768
Fragments arranged by size:
1611 768 73 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 574 2108 2478
Size: 574 1534 370
Fragments arranged by size:
1534 574 370
TatI w'GTAC_w
Cuts at: 0 440 2478
Size: 440 2038
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 80 112 727 759 2478
Size: 80 32 615 32 1719
Fragments arranged by size:
1719 615 80 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 248 1506 1644 2478
Size: 248 1258 138 834
Fragments arranged by size:
1258 834 248 138
ZraI GAC'GTC
Cuts at: 0 2137 2478
Size: 2137 341
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AhdI AleI
AlfI AlwNI ApaI AscI AvaI BamHI BanI BanII
BbsI BbvCI BciVI BglI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DrdI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FseI
FspI FspAI HaeII Hin4I HincII MscI MslI MspA1I
NaeI NcoI NgoMIV PasI PflMI PfoI PmlI PpuMI
PshAI PspOMI PvuII SacI SapI SfcI SfiI SmlI
StyI TaqII TatI TstI XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AloI ApaLI ApoI AseI
AsiSI AvrII BaeI BbeI BcgI BclI BfrBI BglII
BlpI BmtI BplI BsaBI BsiWI BsrDI BsrGI BstBI
BstEII BstZ17I DraI DraIII EciI EcoRI EcoRV FalI
HindIII HpaI KasI KpnI MfeI MluI MmeI NarI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PmeI PpiI PsiI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XhoI XmaI XmnI