Restriction map for
pFN21AE2109
(Linear) (Six-base) MAPSORT of: KIEE2109.seq Check: 3297 from: 1 to: 2790
KIEE2109.orf
With 182 enzymes: SgfI *
July 15, 2010 18:55 ..
AccI GT'mk_AC
Cuts at: 0 1040 1322 2777 2790
Size: 1040 282 1455 13
Fragments arranged by size:
1455 1040 282 13
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 1806 2099 2434 2735 2790
Size: 569 1237 293 335 301 55
Fragments arranged by size:
1237 569 335 301 293 55
AfeI AGC'GCT
Cuts at: 0 905 2790
Size: 905 1885
AflII C'TTAA_G
Cuts at: 0 249 2790
Size: 249 2541
AhdI GACnn_n'nnGTC
Cuts at: 0 635 647 2790
Size: 635 12 2143
Fragments arranged by size:
2143 635 12
AleI CACnn'nnGTG
Cuts at: 0 2275 2790
Size: 2275 515
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 256 2575 2790
Size: 256 2319 215
Fragments arranged by size:
2319 256 215
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2364 2396 2790
Size: 2364 32 394
Fragments arranged by size:
2364 394 32
AlwNI CAG_nnn'CTG
Cuts at: 0 480 1437 2043 2173 2290 2549 2790
Size: 480 957 606 130 117 259 241
Fragments arranged by size:
957 606 480 259 241 130 117
ApaI G_GGCC'C
Cuts at: 0 699 1167 1258 1424 2790
Size: 699 468 91 166 1366
Fragments arranged by size:
1366 699 468 166 91
ApoI r'AATT_y
Cuts at: 0 1889 2790
Size: 1889 901
AvaI C'yCGr_G
Cuts at: 0 766 1308 1417 2107 2643 2679 2790
Size: 766 542 109 690 536 36 111
Fragments arranged by size:
766 690 542 536 111 109 36
AvrII C'CTAG_G
Cuts at: 0 58 2790
Size: 58 2732
BanI G'GyrC_C
Cuts at: 0 1092 2559 2790
Size: 1092 1467 231
Fragments arranged by size:
1467 1092 231
BanII G_rGCy'C
Cuts at: 0 99 393 699 1167 1258 1418 1424 1550
Size: 99 294 306 468 91 160 6 126
Cuts at: 1550 2003 2239 2790
Size: 453 236 551
Fragments arranged by size:
551 468 453 306 294 236 160 126
99 91 6
BciVI GTATCCnnnnn_n'
Cuts at: 0 2470 2790
Size: 2470 320
BglI GCCn_nnn'nGGC
Cuts at: 0 1109 1253 1775 2790
Size: 1109 144 522 1015
Fragments arranged by size:
1109 1015 522 144
Bme1580I G_kGCm'C
Cuts at: 0 699 1097 1167 1258 1424 2790
Size: 699 398 70 91 166 1366
Fragments arranged by size:
1366 699 398 166 91 70
BmgBI CAC'GTC
Cuts at: 0 965 974 2790
Size: 965 9 1816
Fragments arranged by size:
1816 965 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 490 2250 2790
Size: 490 1760 540
Fragments arranged by size:
1760 540 490
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 482 1148 1189 1213 1318 1815 2124
Size: 329 153 666 41 24 105 497 309
Cuts at: 2124 2198 2790
Size: 74 592
Fragments arranged by size:
666 592 497 329 309 153 105 74
41 24
Bpu10I CC'TnA_GC
Cuts at: 0 754 2790
Size: 754 2036
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 645 654 1735 2790
Size: 163 482 9 1081 1055
Fragments arranged by size:
1081 1055 482 163 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 516 2790
Size: 516 2274
BsaHI Gr'CG_yC
Cuts at: 0 622 2790
Size: 622 2168
BsaWI w'CCGG_w
Cuts at: 0 32 2790
Size: 32 2758
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2233 2236 2263 2266 2364 2394 2790
Size: 2233 3 27 3 98 30 396
Fragments arranged by size:
2233 396 98 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 197 209 1315 1340 1558 2241 2309 2760
Size: 197 12 1106 25 218 683 68 451
Cuts at: 2760 2790
Size: 30
Fragments arranged by size:
1106 683 451 218 197 68 30 25
12
BseYI C'CCAG_C
Cuts at: 0 1079 1430 2173 2390 2790
Size: 1079 351 743 217 400
Fragments arranged by size:
1079 743 400 351 217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1258 2056 2790
Size: 1258 798 734
Fragments arranged by size:
1258 798 734
BsiEI CG_ry'CG
Cuts at: 0 1274 2790
Size: 1274 1516
BsiHKAI G_wGCw'C
Cuts at: 0 1175 1199 1418 1550 2003 2790
Size: 1175 24 219 132 453 787
Fragments arranged by size:
1175 787 453 219 132 24
BsmI GAATG_Cn'
Cuts at: 0 564 1073 2790
Size: 564 509 1717
Fragments arranged by size:
1717 564 509
Bsp1286I G_dGCh'C
Cuts at: 0 99 393 699 1097 1167 1175 1199 1258
Size: 99 294 306 398 70 8 24 59
Cuts at: 1258 1418 1424 1550 2003 2239 2790
Size: 160 6 126 453 236 551
Fragments arranged by size:
551 453 398 306 294 236 160 126
99 70 59 24 8 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2021 2790
Size: 2021 769
BsrBI CCG'CTC
Cuts at: 0 890 1344 2790
Size: 890 454 1446
Fragments arranged by size:
1446 890 454
BsrDI GCAATG_nn'
Cuts at: 0 560 1497 2790
Size: 560 937 1293
Fragments arranged by size:
1293 937 560
BsrFI r'CCGG_y
Cuts at: 0 1011 1143 1269 1273 1615 2049 2790
Size: 1011 132 126 4 342 434 741
Fragments arranged by size:
1011 741 434 342 132 126 4
BsrGI T'GTAC_A
Cuts at: 0 2226 2790
Size: 2226 564
BssSI C'ACGA_G
Cuts at: 0 1174 1549 2790
Size: 1174 375 1241
Fragments arranged by size:
1241 1174 375
BstAPI GCAn_nnn'nTGC
Cuts at: 0 160 559 1248 1499 2043 2308 2790
Size: 160 399 689 251 544 265 482
Fragments arranged by size:
689 544 482 399 265 251 160
BstEII G'GTnAC_C
Cuts at: 0 297 450 2790
Size: 297 153 2340
Fragments arranged by size:
2340 297 153
BstXI CCAn_nnnn'nTGG
Cuts at: 0 978 2470 2790
Size: 978 1492 320
Fragments arranged by size:
1492 978 320
BstYI r'GATC_y
Cuts at: 0 747 1754 2790
Size: 747 1007 1036
Fragments arranged by size:
1036 1007 747
Bsu36I CC'TnA_GG
Cuts at: 0 189 2790
Size: 189 2601
BtgI C'CryG_G
Cuts at: 0 717 2087 2469 2594 2744 2790
Size: 717 1370 382 125 150 46
Fragments arranged by size:
1370 717 382 150 125 46
BtsI GCAGTG_nn'
Cuts at: 0 2559 2790
Size: 2559 231
DraI TTT'AAA
Cuts at: 0 335 2790
Size: 335 2455
DraIII CAC_nnn'GTG
Cuts at: 0 1233 2790
Size: 1233 1557
DrdI GACnn_nn'nnGTC
Cuts at: 0 777 2581 2790
Size: 777 1804 209
Fragments arranged by size:
1804 777 209
EaeI y'GGCC_r
Cuts at: 0 1013 1271 2051 2790
Size: 1013 258 780 739
Fragments arranged by size:
1013 780 739 258
EagI C'GGCC_G
Cuts at: 0 1271 2790
Size: 1271 1519
EarI CTCTTCn'nnn_
Cuts at: 0 892 2445 2790
Size: 892 1553 345
Fragments arranged by size:
1553 892 345
EcoICRI GAG'CTC
Cuts at: 0 1416 1548 2001 2790
Size: 1416 132 453 789
Fragments arranged by size:
1416 789 453 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 482 569 1148 1189 1213 1318 1806
Size: 329 153 87 579 41 24 105 488
Cuts at: 1806 1815 2099 2124 2198 2434 2735 2790
Size: 9 284 25 74 236 301 55
Fragments arranged by size:
579 488 329 301 284 236 153 105
87 74 55 41 25 24 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 383 1734 2213 2790
Size: 383 1351 479 577
Fragments arranged by size:
1351 577 479 383
EcoO109I rG'GnC_Cy
Cuts at: 0 55 596 695 696 875 1163 1164 1255
Size: 55 541 99 1 179 288 1 91
Cuts at: 1255 1369 1421 2790
Size: 114 52 1369
Fragments arranged by size:
1369 541 288 179 114 99 91 55
52 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 409 1776 2790
Size: 409 1367 1014
Fragments arranged by size:
1367 1014 409
FseI GG_CCGG'CC
Cuts at: 0 1277 2790
Size: 1277 1513
FspI TGC'GCA
Cuts at: 0 1397 2790
Size: 1397 1393
FspAI rTGC'GCAy
Cuts at: 0 1397 2790
Size: 1397 1393
HaeII r_GCGC'y
Cuts at: 0 907 1141 1526 2790
Size: 907 234 385 1264
Fragments arranged by size:
1264 907 385 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 192 224 254 281 286 313 470 502
Size: 192 32 30 27 5 27 157 32
Cuts at: 502 580 612 621 633 653 665 2790
Size: 78 32 9 12 20 12 2125
Fragments arranged by size:
2125 192 157 78 32 32 32 30
27 27 20 12 12 9 5
HincII GTy'rAC
Cuts at: 0 1224 2790
Size: 1224 1566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 2790
Size: 737 2053
MslI CAynn'nnrTG
Cuts at: 0 1234 1391 2275 2468 2653 2790
Size: 1234 157 884 193 185 137
Fragments arranged by size:
1234 884 193 185 157 137
MspA1I CmG'CkG
Cuts at: 0 434 921 1070 1191 1437 1508 1854 2170
Size: 434 487 149 121 246 71 346 316
Cuts at: 2170 2275 2487 2708 2753 2790
Size: 105 212 221 45 37
Fragments arranged by size:
487 434 346 316 246 221 212 149
121 105 71 45 37
NaeI GCC'GGC
Cuts at: 0 1013 1145 1271 1275 2051 2790
Size: 1013 132 126 4 776 739
Fragments arranged by size:
1013 776 739 132 126 4
NcoI C'CATG_G
Cuts at: 0 2469 2594 2790
Size: 2469 125 196
Fragments arranged by size:
2469 196 125
NgoMIV G'CCGG_C
Cuts at: 0 1011 1143 1269 1273 2049 2790
Size: 1011 132 126 4 776 741
Fragments arranged by size:
1011 776 741 132 126 4
NspI r_CATG'y
Cuts at: 0 318 524 1185 1942 2790
Size: 318 206 661 757 848
Fragments arranged by size:
848 757 661 318 206
PasI CC'CwG_GG
Cuts at: 0 684 2790
Size: 684 2106
PflMI CCAn_nnn'nTGG
Cuts at: 0 379 1233 1437 2790
Size: 379 854 204 1353
Fragments arranged by size:
1353 854 379 204
PfoI T'CCnGG_A
Cuts at: 0 103 1893 2214 2790
Size: 103 1790 321 576
Fragments arranged by size:
1790 576 321 103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2364 2396 2790
Size: 2364 32 394
Fragments arranged by size:
2364 394 32
PpuMI rG'GwC_Cy
Cuts at: 0 596 875 2790
Size: 596 279 1915
Fragments arranged by size:
1915 596 279
PshAI GACnn'nnGTC
Cuts at: 0 2753 2790
Size: 2753 37
PspOMI G'GGCC_C
Cuts at: 0 695 1163 1254 1420 2790
Size: 695 468 91 166 1370
Fragments arranged by size:
1370 695 468 166 91
PspXI vC'TCGA_Gb
Cuts at: 0 2679 2790
Size: 2679 111
PstI C_TGCA'G
Cuts at: 0 1211 1522 2048 2492 2554 2790
Size: 1211 311 526 444 62 236
Fragments arranged by size:
1211 526 444 311 236 62
PvuII CAG'CTG
Cuts at: 0 434 1070 1191 1437 2170 2275 2487 2790
Size: 434 636 121 246 733 105 212 303
Fragments arranged by size:
733 636 434 303 246 212 121 105
SacI G_AGCT'C
Cuts at: 0 1418 1550 2003 2790
Size: 1418 132 453 787
Fragments arranged by size:
1418 787 453 132
SexAI A'CCwGG_T
Cuts at: 0 592 871 2790
Size: 592 279 1919
Fragments arranged by size:
1919 592 279
SfcI C'TryA_G
Cuts at: 0 515 690 1207 1518 2044 2488 2550 2790
Size: 515 175 517 311 526 444 62 240
Fragments arranged by size:
526 517 515 444 311 240 175 62
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1253 2790
Size: 1253 1537
SmaI CCC'GGG
Cuts at: 0 768 1310 2109 2790
Size: 768 542 799 681
Fragments arranged by size:
799 768 681 542
SmlI C'TyrA_G
Cuts at: 0 178 249 633 660 1750 2679 2790
Size: 178 71 384 27 1090 929 111
Fragments arranged by size:
1090 929 384 178 111 71 27
SphI G_CATG'C
Cuts at: 0 524 1185 2790
Size: 524 661 1605
Fragments arranged by size:
1605 661 524
StuI AGG'CCT
Cuts at: 0 1133 1217 1729 1819 2790
Size: 1133 84 512 90 971
Fragments arranged by size:
1133 971 512 90 84
StyI C'CwwG_G
Cuts at: 0 58 977 1365 2469 2594 2790
Size: 58 919 388 1104 125 196
Fragments arranged by size:
1104 919 388 196 125 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 316 1303 2790
Size: 316 987 1487
Fragments arranged by size:
1487 987 316
TatI w'GTAC_w
Cuts at: 0 2226 2790
Size: 2226 564
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 54 86 126 158 444 476 2233 2265
Size: 54 32 40 32 286 32 1757 32
Cuts at: 2265 2401 2433 2790
Size: 136 32 357
Fragments arranged by size:
1757 357 286 136 54 40 32 32
32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 978 2476 2790
Size: 978 1498 314
Fragments arranged by size:
1498 978 314
XhoI C'TCGA_G
Cuts at: 0 2679 2790
Size: 2679 111
XmaI C'CCGG_G
Cuts at: 0 766 1308 2107 2790
Size: 766 542 799 683
Fragments arranged by size:
799 766 683 542
XmnI GAAnn'nnTTC
Cuts at: 0 1819 2790
Size: 1819 971
Enzymes that do cut:
AccI AcuI AfeI AflII AhdI AleI AlfI AloI
AlwNI ApaI ApoI AvaI AvrII BanI BanII BciVI
BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraI
DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI FseI FspI FspAI HaeII Hin4I HincII
MmeI MslI MspA1I NaeI NcoI NgoMIV NspI PasI
PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuII SacI SexAI SfcI SfiI SmaI SmlI SphI
StuI StyI TaqII TatI TstI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AgeI ApaLI AscI
AseI AsiSI BaeI BamHI BbeI BbsI BbvCI BcgI
BclI BfrBI BglII BlpI BmtI BplI BsaAI BsaBI
BsiWI BsmBI BspEI BspHI BssHII BstBI BstZ17I BtgZI
ClaI CspCI EciI EcoRI EcoRV HindIII HpaI KasI
KpnI MfeI MluI MscI NarI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PsiI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI ZraI