Restriction map for
pFN21AE2108
(Linear) (Six-base) MAPSORT of: KIEE2108.seq Check: 8483 from: 1 to: 2535
KIEE2108.orf
With 182 enzymes: SgfI *
July 15, 2010 18:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 487 2535
Size: 487 2048
AatII G_ACGT'C
Cuts at: 0 1776 2535
Size: 1776 759
AccI GT'mk_AC
Cuts at: 0 1509 1970 2466 2535
Size: 1509 461 496 69
Fragments arranged by size:
1509 496 461 69
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1464 2459 2535
Size: 1464 995 76
Fragments arranged by size:
1464 995 76
AflIII A'CryG_T
Cuts at: 0 211 457 1512 1865 2489 2535
Size: 211 246 1055 353 624 46
Fragments arranged by size:
1055 624 353 246 211 46
AhdI GACnn_n'nnGTC
Cuts at: 0 1695 2535
Size: 1695 840
AlwNI CAG_nnn'CTG
Cuts at: 0 1037 2535
Size: 1037 1498
ApaI G_GGCC'C
Cuts at: 0 961 2535
Size: 961 1574
ApaLI G'TGCA_C
Cuts at: 0 117 2535
Size: 117 2418
ApoI r'AATT_y
Cuts at: 0 967 1223 2014 2535
Size: 967 256 791 521
Fragments arranged by size:
967 791 521 256
AseI AT'TA_AT
Cuts at: 0 2174 2535
Size: 2174 361
AvaI C'yCGr_G
Cuts at: 0 56 2535
Size: 56 2479
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2255 2288 2476 2509 2535
Size: 2255 33 188 33 26
Fragments arranged by size:
2255 188 33 33 26
BanI G'GyrC_C
Cuts at: 0 51 67 361 580 2005 2535
Size: 51 16 294 219 1425 530
Fragments arranged by size:
1425 530 294 219 51 16
BanII G_rGCy'C
Cuts at: 0 961 1052 2535
Size: 961 91 1483
Fragments arranged by size:
1483 961 91
BbeI G_GCGC'C
Cuts at: 0 55 71 365 584 2535
Size: 55 16 294 219 1951
Fragments arranged by size:
1951 294 219 55 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 173 214 2129 2535
Size: 173 41 1915 406
Fragments arranged by size:
1915 406 173 41
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 625 659 2535
Size: 625 34 1876
Fragments arranged by size:
1876 625 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 391 907 2535
Size: 391 516 1628
Fragments arranged by size:
1628 516 391
BclI T'GATC_A
Cuts at: 0 2178 2535
Size: 2178 357
BfrBI ATG'CAT
Cuts at: 0 992 2535
Size: 992 1543
BglI GCCn_nnn'nGGC
Cuts at: 0 2314 2535
Size: 2314 221
Bme1580I G_kGCm'C
Cuts at: 0 7 121 567 961 2535
Size: 7 114 446 394 1574
Fragments arranged by size:
1574 446 394 114 7
BmgBI CAC'GTC
Cuts at: 0 8 212 2535
Size: 8 204 2323
Fragments arranged by size:
2323 204 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 379 1731 2204 2535
Size: 379 1352 473 331
Fragments arranged by size:
1352 473 379 331
BmtI G_CTAG'C
Cuts at: 0 2035 2535
Size: 2035 500
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1692 2466 2535
Size: 1692 774 69
Fragments arranged by size:
1692 774 69
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 468 486 697 1059 1942 2535
Size: 414 54 18 211 362 883 593
Fragments arranged by size:
883 593 414 362 211 54 18
BsaAI yAC'GTr
Cuts at: 0 2492 2535
Size: 2492 43
BsaBI GATnn'nnATC
Cuts at: 0 278 2525 2535
Size: 278 2247 10
Fragments arranged by size:
2247 278 10
BsaHI Gr'CG_yC
Cuts at: 0 52 68 362 581 1773 2535
Size: 52 16 294 219 1192 762
Fragments arranged by size:
1192 762 294 219 52 16
BsaWI w'CCGG_w
Cuts at: 0 73 2535
Size: 73 2462
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 597 2535
Size: 597 1938
BseYI C'CCAG_C
Cuts at: 0 47 256 626 1150 2496 2535
Size: 47 209 370 524 1346 39
Fragments arranged by size:
1346 524 370 209 47 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 996 1166 2535
Size: 618 378 170 1369
Fragments arranged by size:
1369 618 378 170
BsiHKAI G_wGCw'C
Cuts at: 0 121 2535
Size: 121 2414
BsmI GAATG_Cn'
Cuts at: 0 994 1081 1201 2535
Size: 994 87 120 1334
Fragments arranged by size:
1334 994 120 87
Bsp1286I G_dGCh'C
Cuts at: 0 7 121 567 961 1052 2535
Size: 7 114 446 394 91 1483
Fragments arranged by size:
1483 446 394 114 91 7
BspHI T'CATG_A
Cuts at: 0 2082 2535
Size: 2082 453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 487 2535
Size: 487 2048
BsrBI CCG'CTC
Cuts at: 0 65 2535
Size: 65 2470
BsrDI GCAATG_nn'
Cuts at: 0 525 1430 1550 2535
Size: 525 905 120 985
Fragments arranged by size:
985 905 525 120
BsrGI T'GTAC_A
Cuts at: 0 1568 2091 2535
Size: 1568 523 444
Fragments arranged by size:
1568 523 444
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1552 2535
Size: 1552 983
BstXI CCAn_nnnn'nTGG
Cuts at: 0 392 1686 2535
Size: 392 1294 849
Fragments arranged by size:
1294 849 392
BstYI r'GATC_y
Cuts at: 0 279 1281 2535
Size: 279 1002 1254
Fragments arranged by size:
1254 1002 279
BtgI C'CryG_G
Cuts at: 0 873 2535
Size: 873 1662
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 554 783 2535
Size: 554 229 1752
Fragments arranged by size:
1752 554 229
BtsI GCAGTG_nn'
Cuts at: 0 189 552 688 807 892 2535
Size: 189 363 136 119 85 1643
Fragments arranged by size:
1643 363 189 136 119 85
DraIII CAC_nnn'GTG
Cuts at: 0 713 2535
Size: 713 1822
DrdI GACnn_nn'nnGTC
Cuts at: 0 2074 2535
Size: 2074 461
EaeI y'GGCC_r
Cuts at: 0 243 489 522 687 1890 2235 2535
Size: 243 246 33 165 1203 345 300
Fragments arranged by size:
1203 345 300 246 243 165 33
EarI CTCTTCn'nnn_
Cuts at: 0 1338 2435 2535
Size: 1338 1097 100
Fragments arranged by size:
1338 1097 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2388 2535
Size: 2388 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 468 486 697 1059 1464 1942 2459
Size: 414 54 18 211 362 405 478 517
Cuts at: 2459 2535
Size: 76
Fragments arranged by size:
517 478 414 405 362 211 76 54
18
EcoO109I rG'GnC_Cy
Cuts at: 0 780 958 2445 2535
Size: 780 178 1487 90
Fragments arranged by size:
1487 780 178 90
EcoRV GAT'ATC
Cuts at: 0 999 1794 2535
Size: 999 795 741
Fragments arranged by size:
999 795 741
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1380 1494 2535
Size: 1380 114 1041
Fragments arranged by size:
1380 1041 114
HaeII r_GCGC'y
Cuts at: 0 25 55 71 365 584 2535
Size: 25 30 16 294 219 1951
Fragments arranged by size:
1951 294 219 30 25 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 893 925 1681 1713 2163 2195 2535
Size: 893 32 756 32 450 32 340
Fragments arranged by size:
893 756 450 340 32 32 32
HincII GTy'rAC
Cuts at: 0 177 813 930 2046 2535
Size: 177 636 117 1116 489
Fragments arranged by size:
1116 636 489 177 117
KasI G'GCGC_C
Cuts at: 0 51 67 361 580 2535
Size: 51 16 294 219 1955
Fragments arranged by size:
1955 294 219 51 16
MfeI C'AATT_G
Cuts at: 0 1490 2535
Size: 1490 1045
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 1410 2463 2535
Size: 680 730 1053 72
Fragments arranged by size:
1053 730 680 72
MscI TGG'CCA
Cuts at: 0 245 524 1892 2535
Size: 245 279 1368 643
Fragments arranged by size:
1368 643 279 245
MslI CAynn'nnrTG
Cuts at: 0 1684 2535
Size: 1684 851
MspA1I CmG'CkG
Cuts at: 0 356 494 626 866 1150 1789 2535
Size: 356 138 132 240 284 639 746
Fragments arranged by size:
746 639 356 284 240 138 132
NarI GG'CG_CC
Cuts at: 0 52 68 362 581 2535
Size: 52 16 294 219 1954
Fragments arranged by size:
1954 294 219 52 16
NcoI C'CATG_G
Cuts at: 0 873 2535
Size: 873 1662
NdeI CA'TA_TG
Cuts at: 0 326 2535
Size: 326 2209
NheI G'CTAG_C
Cuts at: 0 2031 2535
Size: 2031 504
NsiI A_TGCA'T
Cuts at: 0 994 2535
Size: 994 1541
NspI r_CATG'y
Cuts at: 0 338 461 1516 1869 2535
Size: 338 123 1055 353 666
Fragments arranged by size:
1055 666 353 338 123
PciI A'CATG_T
Cuts at: 0 457 1512 1865 2535
Size: 457 1055 353 670
Fragments arranged by size:
1055 670 457 353
PflMI CCAn_nnn'nTGG
Cuts at: 0 263 577 626 1583 1699 2535
Size: 263 314 49 957 116 836
Fragments arranged by size:
957 836 314 263 116 49
PfoI T'CCnGG_A
Cuts at: 0 391 502 1206 2535
Size: 391 111 704 1329
Fragments arranged by size:
1329 704 391 111
PpuMI rG'GwC_Cy
Cuts at: 0 780 2445 2535
Size: 780 1665 90
Fragments arranged by size:
1665 780 90
PsiI TTA'TAA
Cuts at: 0 1828 2535
Size: 1828 707
PspOMI G'GGCC_C
Cuts at: 0 957 2535
Size: 957 1578
PstI C_TGCA'G
Cuts at: 0 538 1033 1219 1548 2535
Size: 538 495 186 329 987
Fragments arranged by size:
987 538 495 329 186
PvuII CAG'CTG
Cuts at: 0 356 626 866 1150 2535
Size: 356 270 240 284 1385
Fragments arranged by size:
1385 356 284 270 240
SanDI GG'GwC_CC
Cuts at: 0 780 2535
Size: 780 1755
ScaI AGT'ACT
Cuts at: 0 1910 2535
Size: 1910 625
SfcI C'TryA_G
Cuts at: 0 534 1029 1215 1544 2535
Size: 534 495 186 329 991
Fragments arranged by size:
991 534 495 329 186
SfoI GGC'GCC
Cuts at: 0 53 69 363 582 2535
Size: 53 16 294 219 1953
Fragments arranged by size:
1953 294 219 53 16
SmaI CCC'GGG
Cuts at: 0 58 2535
Size: 58 2477
SpeI A'CTAG_T
Cuts at: 0 1389 2535
Size: 1389 1146
SspI AAT'ATT
Cuts at: 0 1955 2535
Size: 1955 580
StyI C'CwwG_G
Cuts at: 0 873 2535
Size: 873 1662
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1554 2535
Size: 1554 981
TatI w'GTAC_w
Cuts at: 0 1568 1908 2091 2423 2535
Size: 1568 340 183 332 112
Fragments arranged by size:
1568 340 332 183 112
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 699 731 1164 1196 1783 1815 1855 1887
Size: 699 32 433 32 587 32 40 32
Cuts at: 1887 2535
Size: 648
Fragments arranged by size:
699 648 587 433 40 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 907 1531 2535
Size: 907 624 1004
Fragments arranged by size:
1004 907 624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 121 739 2535
Size: 121 618 1796
Fragments arranged by size:
1796 618 121
XmaI C'CCGG_G
Cuts at: 0 56 2535
Size: 56 2479
XmnI GAAnn'nnTTC
Cuts at: 0 1447 1762 2535
Size: 1447 315 773
Fragments arranged by size:
1447 773 315
ZraI GAC'GTC
Cuts at: 0 1774 2535
Size: 1774 761
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AhdI AlwNI ApaI
ApaLI ApoI AseI AvaI BaeI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI Bme1580I BmgBI
BmrI BmtI BplI BpmI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrGI BstAPI BstXI BstYI BtgI BtgZI
BtsI DraIII DrdI EaeI EarI EciI Eco57MI EcoO109I
EcoRV FalI HaeII Hin4I HincII KasI MfeI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NsiI
NspI PciI PflMI PfoI PpuMI PsiI PspOMI PstI
PvuII SanDI ScaI SfcI SfoI SmaI SpeI SspI
StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AleI AlfI AloI
AscI AsiSI AvrII BamHI BbvCI BglII BlpI Bpu10I
BpuEI BsaI BsaXI BsiEI BsiWI BsmBI BspEI BsrFI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI EagI EcoICRI EcoNI EcoRI FseI FspI FspAI
HindIII HpaI KpnI MluI NaeI NgoMIV NotI NruI
PacI PasI PmeI PmlI PpiI PshAI PspXI PsrI
PvuI RsrII SacI SacII SalI SapI SbfI SexAI
SfiI SgfI SgrAI SmlI SnaBI SphI SrfI StuI
SwaI XbaI XhoI