Restriction map for
pFN21AE2103
(Linear) (Six-base) MAPSORT of: KIEE2103.seq Check: 4490 from: 1 to: 2766
KIEE2103.orf
With 182 enzymes: SgfI *
March 22, 2012 15:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 724 2766
Size: 724 2042
AatII G_ACGT'C
Cuts at: 0 146 2766
Size: 146 2620
AccI GT'mk_AC
Cuts at: 0 629 664 2687 2703 2766
Size: 629 35 2023 16 63
Fragments arranged by size:
2023 629 63 35 16
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1366 1489 2766
Size: 1366 123 1277
Fragments arranged by size:
1366 1277 123
AflIII A'CryG_T
Cuts at: 0 2305 2389 2766
Size: 2305 84 377
Fragments arranged by size:
2305 377 84
AleI CACnn'nnGTG
Cuts at: 0 1390 2766
Size: 1390 1376
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 902 1357 2766
Size: 902 455 1409
Fragments arranged by size:
1409 902 455
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2549 2581 2766
Size: 2549 32 185
Fragments arranged by size:
2549 185 32
AlwNI CAG_nnn'CTG
Cuts at: 0 822 2766
Size: 822 1944
ApaLI G'TGCA_C
Cuts at: 0 169 1566 2766
Size: 169 1397 1200
Fragments arranged by size:
1397 1200 169
ApoI r'AATT_y
Cuts at: 0 2346 2766
Size: 2346 420
AvaI C'yCGr_G
Cuts at: 0 151 550 1467 2330 2341 2766
Size: 151 399 917 863 11 425
Fragments arranged by size:
917 863 425 399 151 11
AvrII C'CTAG_G
Cuts at: 0 645 2766
Size: 645 2121
BamHI G'GATC_C
Cuts at: 0 224 311 949 2766
Size: 224 87 638 1817
Fragments arranged by size:
1817 638 224 87
BanI G'GyrC_C
Cuts at: 0 546 594 2428 2766
Size: 546 48 1834 338
Fragments arranged by size:
1834 546 338 48
BanII G_rGCy'C
Cuts at: 0 2675 2766
Size: 2675 91
BbsI GAAGACnn'nnnn_
Cuts at: 0 1157 1526 1606 1776 2766
Size: 1157 369 80 170 990
Fragments arranged by size:
1157 990 369 170 80
BbvCI CC'TCA_GC
Cuts at: 0 10 2766
Size: 10 2756
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1706 1740 2766
Size: 1706 34 1026
Fragments arranged by size:
1706 1026 34
BfrBI ATG'CAT
Cuts at: 0 538 2766
Size: 538 2228
BglII A'GATC_T
Cuts at: 0 2256 2766
Size: 2256 510
Bme1580I G_kGCm'C
Cuts at: 0 173 197 1570 2766
Size: 173 24 1373 1196
Fragments arranged by size:
1373 1196 173 24
BmgBI CAC'GTC
Cuts at: 0 399 2766
Size: 399 2367
BmrI ACTGGGnnnn_n'
Cuts at: 0 747 1110 2633 2766
Size: 747 363 1523 133
Fragments arranged by size:
1523 747 363 133
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1776 2254 2766
Size: 1776 478 512
Fragments arranged by size:
1776 512 478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 822 1349 1373 1771 2766
Size: 82 740 527 24 398 995
Fragments arranged by size:
995 740 527 398 82 24
Bpu10I CC'TnA_GC
Cuts at: 0 10 2219 2766
Size: 10 2209 547
Fragments arranged by size:
2209 547 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2224 2766
Size: 2224 542
BsaI GGTCTCn'nnnn_
Cuts at: 0 486 2766
Size: 486 2280
BsaHI Gr'CG_yC
Cuts at: 0 143 2766
Size: 143 2623
BsaWI w'CCGG_w
Cuts at: 0 128 1720 2766
Size: 128 1592 1046
Fragments arranged by size:
1592 1046 128
BseYI C'CCAG_C
Cuts at: 0 184 300 944 2508 2766
Size: 184 116 644 1564 258
Fragments arranged by size:
1564 644 258 184 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 952 2766
Size: 390 562 1814
Fragments arranged by size:
1814 562 390
BsiHKAI G_wGCw'C
Cuts at: 0 71 173 1570 2675 2766
Size: 71 102 1397 1105 91
Fragments arranged by size:
1397 1105 102 91 71
BsmI GAATG_Cn'
Cuts at: 0 2288 2766
Size: 2288 478
Bsp1286I G_dGCh'C
Cuts at: 0 71 173 197 1570 2675 2766
Size: 71 102 24 1373 1105 91
Fragments arranged by size:
1373 1105 102 91 71 24
BspEI T'CCGG_A
Cuts at: 0 128 2766
Size: 128 2638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 724 2766
Size: 724 2042
BsrBI CCG'CTC
Cuts at: 0 2434 2766
Size: 2434 332
BsrDI GCAATG_nn'
Cuts at: 0 746 1365 1683 1799 1805 2766
Size: 746 619 318 116 6 961
Fragments arranged by size:
961 746 619 318 116 6
BsrFI r'CCGG_y
Cuts at: 0 423 566 2766
Size: 423 143 2200
Fragments arranged by size:
2200 423 143
BsrGI T'GTAC_A
Cuts at: 0 2381 2534 2724 2766
Size: 2381 153 190 42
Fragments arranged by size:
2381 190 153 42
BssHII G'CGCG_C
Cuts at: 0 77 94 305 2766
Size: 77 17 211 2461
Fragments arranged by size:
2461 211 77 17
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1246 2766
Size: 1246 1520
BstEII G'GTnAC_C
Cuts at: 0 1471 1648 2766
Size: 1471 177 1118
Fragments arranged by size:
1471 1118 177
BstYI r'GATC_y
Cuts at: 0 224 311 518 881 949 1281 2256 2766
Size: 224 87 207 363 68 332 975 510
Fragments arranged by size:
975 510 363 332 224 207 87 68
BstZ17I GTA'TAC
Cuts at: 0 2704 2766
Size: 2704 62
Bsu36I CC'TnA_GG
Cuts at: 0 505 2766
Size: 505 2261
BtgI C'CryG_G
Cuts at: 0 623 1022 2766
Size: 623 399 1744
Fragments arranged by size:
1744 623 399
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 256 1658 2766
Size: 256 1402 1108
Fragments arranged by size:
1402 1108 256
BtsI GCAGTG_nn'
Cuts at: 0 964 1495 1534 1964 2766
Size: 964 531 39 430 802
Fragments arranged by size:
964 802 531 430 39
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1800 1835 2766
Size: 1800 35 931
Fragments arranged by size:
1800 931 35
EaeI y'GGCC_r
Cuts at: 0 99 915 1826 2766
Size: 99 816 911 940
Fragments arranged by size:
940 911 816 99
EarI CTCTTCn'nnn_
Cuts at: 0 517 2766
Size: 517 2249
EcoICRI GAG'CTC
Cuts at: 0 2673 2766
Size: 2673 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 822 1349 1366 1373 1489 1771 2766
Size: 82 740 527 17 7 116 282 995
Fragments arranged by size:
995 740 527 282 116 82 17 7
EcoO109I rG'GnC_Cy
Cuts at: 0 181 642 2766
Size: 181 461 2124
Fragments arranged by size:
2124 461 181
EcoRV GAT'ATC
Cuts at: 0 984 2766
Size: 984 1782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2601 2766
Size: 2601 165
HaeII r_GCGC'y
Cuts at: 0 376 573 2509 2766
Size: 376 197 1936 257
Fragments arranged by size:
1936 376 257 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 568 600 2766
Size: 568 32 2166
Fragments arranged by size:
2166 568 32
HincII GTy'rAC
Cuts at: 0 1127 2766
Size: 1127 1639
HindIII A'AGCT_T
Cuts at: 0 2583 2766
Size: 2583 183
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 432 1223 1606 2766
Size: 419 13 791 383 1160
Fragments arranged by size:
1160 791 419 383 13
MscI TGG'CCA
Cuts at: 0 917 1828 2766
Size: 917 911 938
Fragments arranged by size:
938 917 911
MslI CAynn'nnrTG
Cuts at: 0 974 1390 1575 2766
Size: 974 416 185 1191
Fragments arranged by size:
1191 974 416 185
MspA1I CmG'CkG
Cuts at: 0 9 188 944 2443 2766
Size: 9 179 756 1499 323
Fragments arranged by size:
1499 756 323 179 9
NcoI C'CATG_G
Cuts at: 0 623 2766
Size: 623 2143
NsiI A_TGCA'T
Cuts at: 0 540 2766
Size: 540 2226
NspI r_CATG'y
Cuts at: 0 1527 1679 2309 2393 2526 2766
Size: 1527 152 630 84 133 240
Fragments arranged by size:
1527 630 240 152 133 84
PasI CC'CwG_GG
Cuts at: 0 1766 2766
Size: 1766 1000
PciI A'CATG_T
Cuts at: 0 2305 2389 2766
Size: 2305 84 377
Fragments arranged by size:
2305 377 84
PflMI CCAn_nnn'nTGG
Cuts at: 0 234 624 2766
Size: 234 390 2142
Fragments arranged by size:
2142 390 234
PfoI T'CCnGG_A
Cuts at: 0 314 821 2766
Size: 314 507 1945
Fragments arranged by size:
1945 507 314
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1493 1525 2766
Size: 1493 32 1241
Fragments arranged by size:
1493 1241 32
PpuMI rG'GwC_Cy
Cuts at: 0 181 2766
Size: 181 2585
PvuII CAG'CTG
Cuts at: 0 9 944 2766
Size: 9 935 1822
Fragments arranged by size:
1822 935 9
SacI G_AGCT'C
Cuts at: 0 2675 2766
Size: 2675 91
SanDI GG'GwC_CC
Cuts at: 0 181 2766
Size: 181 2585
SapI GCTCTTCn'nnn_
Cuts at: 0 517 2766
Size: 517 2249
SmaI CCC'GGG
Cuts at: 0 1469 2766
Size: 1469 1297
SmlI C'TyrA_G
Cuts at: 0 2239 2766
Size: 2239 527
StyI C'CwwG_G
Cuts at: 0 176 623 645 2276 2766
Size: 176 447 22 1631 490
Fragments arranged by size:
1631 490 447 176 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 468 918 2190 2683 2766
Size: 468 450 1272 493 83
Fragments arranged by size:
1272 493 468 450 83
TatI w'GTAC_w
Cuts at: 0 2308 2381 2534 2724 2766
Size: 2308 73 153 190 42
Fragments arranged by size:
2308 190 153 73 42
Tth111I GACn'n_nGTC
Cuts at: 0 1240 2766
Size: 1240 1526
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 297 1746 1821 1950 2766
Size: 297 1449 75 129 816
Fragments arranged by size:
1449 816 297 129 75
XmaI C'CCGG_G
Cuts at: 0 1467 2766
Size: 1467 1299
XmnI GAAnn'nnTTC
Cuts at: 0 1194 2766
Size: 1194 1572
ZraI GAC'GTC
Cuts at: 0 144 2766
Size: 144 2622
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlfI AloI
AlwNI ApaLI ApoI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BfrBI BglII Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BstAPI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI CspCI EaeI EarI EcoICRI Eco57MI
EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII MmeI
MscI MslI MspA1I NcoI NsiI NspI PasI PciI
PflMI PfoI PpiI PpuMI PvuII SacI SanDI SapI
SmaI SmlI StyI TaqII TatI Tth111I XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI ApaI AscI
AseI AsiSI BaeI BbeI BciVI BclI BglI BlpI
BmtI BsaAI BsaBI BsaXI BseRI BsiEI BsiWI BsmBI
BspHI BssSI BstBI BstXI ClaI DraI DraIII DrdI
EagI EciI EcoNI EcoRI FseI FspI FspAI HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PmeI PmlI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
XbaI XhoI