Restriction map for
pFN21AE2101
(Linear) (Six-base) MAPSORT of: KIEE2101.seq Check: 488 from: 1 to: 2586
KIEE2101.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AccI GT'mk_AC
Cuts at: 0 672 2586
Size: 672 1914
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 458 569 791 1340 1563 2554 2586
Size: 43 415 111 222 549 223 991 32
Fragments arranged by size:
991 549 415 223 222 111 43 32
AflII C'TTAA_G
Cuts at: 0 85 2586
Size: 85 2501
AflIII A'CryG_T
Cuts at: 0 463 2586
Size: 463 2123
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2279 2586
Size: 2279 307
AlwNI CAG_nnn'CTG
Cuts at: 0 264 562 682 1500 2586
Size: 264 298 120 818 1086
Fragments arranged by size:
1086 818 298 264 120
ApoI r'AATT_y
Cuts at: 0 1174 1352 1834 1924 2006 2165 2434 2586
Size: 1174 178 482 90 82 159 269 152
Fragments arranged by size:
1174 482 269 178 159 152 90 82
AseI AT'TA_AT
Cuts at: 0 790 2586
Size: 790 1796
AvaI C'yCGr_G
Cuts at: 0 2117 2586
Size: 2117 469
AvrII C'CTAG_G
Cuts at: 0 826 2586
Size: 826 1760
BbsI GAAGACnn'nnnn_
Cuts at: 0 163 1283 1605 2586
Size: 163 1120 322 981
Fragments arranged by size:
1120 981 322 163
BglII A'GATC_T
Cuts at: 0 1590 2586
Size: 1590 996
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2132 2586
Size: 2132 454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 833 2586
Size: 483 350 1753
Fragments arranged by size:
1753 483 350
Bpu10I CC'TnA_GC
Cuts at: 0 819 2586
Size: 819 1767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 833 1882 2586
Size: 833 1049 704
Fragments arranged by size:
1049 833 704
BsaAI yAC'GTr
Cuts at: 0 126 670 2586
Size: 126 544 1916
Fragments arranged by size:
1916 544 126
BsaWI w'CCGG_w
Cuts at: 0 1058 1481 2586
Size: 1058 423 1105
Fragments arranged by size:
1105 1058 423
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 567 597 837 867 2586
Size: 567 30 240 30 1719
Fragments arranged by size:
1719 567 240 30 30
BseYI C'CCAG_C
Cuts at: 0 965 2174 2586
Size: 965 1209 412
Fragments arranged by size:
1209 965 412
BsiHKAI G_wGCw'C
Cuts at: 0 529 2586
Size: 529 2057
BsmI GAATG_Cn'
Cuts at: 0 2234 2586
Size: 2234 352
Bsp1286I G_dGCh'C
Cuts at: 0 529 2586
Size: 529 2057
BspEI T'CCGG_A
Cuts at: 0 1058 2586
Size: 1058 1528
BspHI T'CATG_A
Cuts at: 0 2028 2586
Size: 2028 558
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2386 2513 2586
Size: 2386 127 73
Fragments arranged by size:
2386 127 73
BsrDI GCAATG_nn'
Cuts at: 0 1112 1192 1233 2427 2586
Size: 1112 80 41 1194 159
Fragments arranged by size:
1194 1112 159 80 41
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2184 2586
Size: 2184 402
BstXI CCAn_nnnn'nTGG
Cuts at: 0 869 1205 2586
Size: 869 336 1381
Fragments arranged by size:
1381 869 336
BstYI r'GATC_y
Cuts at: 0 502 1398 1590 2586
Size: 502 896 192 996
Fragments arranged by size:
996 896 502 192
BtgI C'CryG_G
Cuts at: 0 1814 1827 2586
Size: 1814 13 759
Fragments arranged by size:
1814 759 13
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2287 2322 2586
Size: 2287 35 264
Fragments arranged by size:
2287 264 35
DraI TTT'AAA
Cuts at: 0 621 2586
Size: 621 1965
EaeI y'GGCC_r
Cuts at: 0 1502 2586
Size: 1502 1084
EarI CTCTTCn'nnn_
Cuts at: 0 1207 2338 2586
Size: 1207 1131 248
Fragments arranged by size:
1207 1131 248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 458 483 569 791 833 1340 1563
Size: 43 415 25 86 222 42 507 223
Cuts at: 1563 2554 2586
Size: 991 32
Fragments arranged by size:
991 507 415 223 222 86 43 42
32 25
EcoO109I rG'GnC_Cy
Cuts at: 0 823 2214 2586
Size: 823 1391 372
Fragments arranged by size:
1391 823 372
EcoRI G'AATT_C
Cuts at: 0 1352 2586
Size: 1352 1234
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1893 2586
Size: 1893 693
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 133 165 481 513 1566 1598 1894
Size: 30 103 32 316 32 1053 32 296
Cuts at: 1894 1926 2016 2048 2252 2284 2586
Size: 32 90 32 204 32 302
Fragments arranged by size:
1053 316 302 296 204 103 90 32
32 32 32 32 32 30
HincII GTy'rAC
Cuts at: 0 981 1008 1248 1998 2586
Size: 981 27 240 750 588
Fragments arranged by size:
981 750 588 240 27
HindIII A'AGCT_T
Cuts at: 0 2141 2586
Size: 2141 445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1872 2586
Size: 1872 714
MscI TGG'CCA
Cuts at: 0 1504 2586
Size: 1504 1082
MslI CAynn'nnrTG
Cuts at: 0 242 1203 2092 2586
Size: 242 961 889 494
Fragments arranged by size:
961 889 494 242
NcoI C'CATG_G
Cuts at: 0 1814 1827 2586
Size: 1814 13 759
Fragments arranged by size:
1814 759 13
NspI r_CATG'y
Cuts at: 0 467 2266 2586
Size: 467 1799 320
Fragments arranged by size:
1799 467 320
PciI A'CATG_T
Cuts at: 0 463 2586
Size: 463 2123
PfoI T'CCnGG_A
Cuts at: 0 1620 2586
Size: 1620 966
PpuMI rG'GwC_Cy
Cuts at: 0 2214 2586
Size: 2214 372
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1468 1500 2586
Size: 1468 32 1086
Fragments arranged by size:
1468 1086 32
PstI C_TGCA'G
Cuts at: 0 2363 2586
Size: 2363 223
SexAI A'CCwGG_T
Cuts at: 0 864 2586
Size: 864 1722
SfcI C'TryA_G
Cuts at: 0 424 1081 2359 2586
Size: 424 657 1278 227
Fragments arranged by size:
1278 657 424 227
SmlI C'TyrA_G
Cuts at: 0 85 848 1897 2117 2586
Size: 85 763 1049 220 469
Fragments arranged by size:
1049 763 469 220 85
SnaBI TAC'GTA
Cuts at: 0 126 2586
Size: 126 2460
SspI AAT'ATT
Cuts at: 0 1786 1962 2055 2182 2586
Size: 1786 176 93 127 404
Fragments arranged by size:
1786 404 176 127 93
StyI C'CwwG_G
Cuts at: 0 506 826 1814 1827 2586
Size: 506 320 988 13 759
Fragments arranged by size:
988 759 506 320 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 675 1599 2586
Size: 675 924 987
Fragments arranged by size:
987 924 675
TatI w'GTAC_w
Cuts at: 0 727 1985 2586
Size: 727 1258 601
Fragments arranged by size:
1258 727 601
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 168 200 567 599 2586
Size: 168 32 367 32 1987
Fragments arranged by size:
1987 367 168 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 116 2586
Size: 116 2470
XbaI T'CTAG_A
Cuts at: 0 628 2586
Size: 628 1958
XhoI C'TCGA_G
Cuts at: 0 2117 2586
Size: 2117 469
XmnI GAAnn'nnTTC
Cuts at: 0 2168 2586
Size: 2168 418
Enzymes that do cut:
AccI AcuI AflII AflIII AlfI AlwNI ApoI AseI
AvaI AvrII BbsI BglII BplI BpmI Bpu10I BpuEI
BsaAI BsaWI BsaXI BseYI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrDI BstAPI BstXI BstYI BtgI CspCI
DraI EaeI EarI Eco57MI EcoO109I EcoRI FalI Hin4I
HincII HindIII MmeI MscI MslI NcoI NspI PciI
PfoI PpuMI PsrI PstI SexAI SfcI SmlI SnaBI
SspI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AloI ApaI ApaLI AscI AsiSI BaeI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BmtI BsaI BsaBI BsaHI
BseRI BsgI BsiEI BsiWI BsmBI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI
ClaI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SpeI SphI SrfI StuI SwaI
XcmI XmaI ZraI