Restriction map for
pFN21AE2097
(Linear) (Six-base) MAPSORT of: KIEE2097.seq Check: 2487 from: 1 to: 2415
KIEE2097.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 268 283 299 1050 2114 2415
Size: 185 83 15 16 751 1064 301
Fragments arranged by size:
1064 751 301 185 83 16 15
AflIII A'CryG_T
Cuts at: 0 1361 2415
Size: 1361 1054
AhdI GACnn_n'nnGTC
Cuts at: 0 204 2415
Size: 204 2211
AleI CACnn'nnGTG
Cuts at: 0 1087 1622 2415
Size: 1087 535 793
Fragments arranged by size:
1087 793 535
AlwNI CAG_nnn'CTG
Cuts at: 0 1648 1659 2415
Size: 1648 11 756
Fragments arranged by size:
1648 756 11
ApaLI G'TGCA_C
Cuts at: 0 1080 2415
Size: 1080 1335
ApoI r'AATT_y
Cuts at: 0 260 987 1400 1800 2415
Size: 260 727 413 400 615
Fragments arranged by size:
727 615 413 400 260
BamHI G'GATC_C
Cuts at: 0 1070 2415
Size: 1070 1345
BanII G_rGCy'C
Cuts at: 0 1769 2415
Size: 1769 646
BbsI GAAGACnn'nnnn_
Cuts at: 0 116 305 335 949 1258 1295 2415
Size: 116 189 30 614 309 37 1120
Fragments arranged by size:
1120 614 309 189 116 37 30
BbvCI CC'TCA_GC
Cuts at: 0 526 1672 2415
Size: 526 1146 743
Fragments arranged by size:
1146 743 526
BciVI GTATCCnnnnn_n'
Cuts at: 0 257 2171 2415
Size: 257 1914 244
Fragments arranged by size:
1914 257 244
BglII A'GATC_T
Cuts at: 0 505 2415
Size: 505 1910
Bme1580I G_kGCm'C
Cuts at: 0 1084 1475 2415
Size: 1084 391 940
Fragments arranged by size:
1084 940 391
BmrI ACTGGGnnnn_n'
Cuts at: 0 2297 2415
Size: 2297 118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 522 1848 2415
Size: 522 1326 567
Fragments arranged by size:
1326 567 522
Bpu10I CC'TnA_GC
Cuts at: 0 526 1244 1672 2415
Size: 526 718 428 743
Fragments arranged by size:
743 718 526 428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 956 2415
Size: 956 1459
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2243 2273 2415
Size: 2243 30 142
Fragments arranged by size:
2243 142 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 626 2014 2415
Size: 626 1388 401
Fragments arranged by size:
1388 626 401
BseYI C'CCAG_C
Cuts at: 0 193 2415
Size: 193 2222
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1142 1600 2042 2415
Size: 1142 458 442 373
Fragments arranged by size:
1142 458 442 373
BsiHKAI G_wGCw'C
Cuts at: 0 323 373 1084 2415
Size: 323 50 711 1331
Fragments arranged by size:
1331 711 323 50
BsmI GAATG_Cn'
Cuts at: 0 424 753 2415
Size: 424 329 1662
Fragments arranged by size:
1662 424 329
Bsp1286I G_dGCh'C
Cuts at: 0 323 373 1084 1475 1769 2415
Size: 323 50 711 391 294 646
Fragments arranged by size:
711 646 391 323 294 50
BspHI T'CATG_A
Cuts at: 0 1119 2415
Size: 1119 1296
BsrDI GCAATG_nn'
Cuts at: 0 893 2415
Size: 893 1522
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1156 2415
Size: 1156 1259
BstYI r'GATC_y
Cuts at: 0 505 1070 2415
Size: 505 565 1345
Fragments arranged by size:
1345 565 505
BtgI C'CryG_G
Cuts at: 0 654 2415
Size: 654 1761
BtsI GCAGTG_nn'
Cuts at: 0 1747 2054 2415
Size: 1747 307 361
Fragments arranged by size:
1747 361 307
ClaI AT'CG_AT
Cuts at: 0 2351 2415
Size: 2351 64
DraI TTT'AAA
Cuts at: 0 218 1253 1392 1967 2415
Size: 218 1035 139 575 448
Fragments arranged by size:
1035 575 448 218 139
EaeI y'GGCC_r
Cuts at: 0 657 2415
Size: 657 1758
EarI CTCTTCn'nnn_
Cuts at: 0 15 161 302 536 686 2415
Size: 15 146 141 234 150 1729
Fragments arranged by size:
1729 234 150 146 141 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 268 283 299 522 1050 1848 2114
Size: 185 83 15 16 223 528 798 266
Cuts at: 2114 2415
Size: 301
Fragments arranged by size:
798 528 301 266 223 185 83 16
15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 729 1054 2415
Size: 729 325 1361
Fragments arranged by size:
1361 729 325
EcoO109I rG'GnC_Cy
Cuts at: 0 1047 1553 1609 2415
Size: 1047 506 56 806
Fragments arranged by size:
1047 806 506 56
EcoRI G'AATT_C
Cuts at: 0 1800 2415
Size: 1800 615
EcoRV GAT'ATC
Cuts at: 0 2079 2415
Size: 2079 336
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1080 1600 2415
Size: 1080 520 815
Fragments arranged by size:
1080 815 520
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 190 222 497 529 1101 1133 1486 1518
Size: 190 32 275 32 572 32 353 32
Cuts at: 1518 2283 2315 2415
Size: 765 32 100
Fragments arranged by size:
765 572 353 275 190 100 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 280 2415
Size: 280 2135
MfeI C'AATT_G
Cuts at: 0 773 2415
Size: 773 1642
MslI CAynn'nnrTG
Cuts at: 0 417 719 725 1087 1622 1855 1899 2113
Size: 417 302 6 362 535 233 44 214
Cuts at: 2113 2415
Size: 302
Fragments arranged by size:
535 417 362 302 302 233 214 44
6
MspA1I CmG'CkG
Cuts at: 0 288 525 656 2415
Size: 288 237 131 1759
Fragments arranged by size:
1759 288 237 131
NdeI CA'TA_TG
Cuts at: 0 1152 1836 1896 1944 2221 2415
Size: 1152 684 60 48 277 194
Fragments arranged by size:
1152 684 277 194 60 48
NspI r_CATG'y
Cuts at: 0 1365 2415
Size: 1365 1050
PciI A'CATG_T
Cuts at: 0 1361 2415
Size: 1361 1054
PflMI CCAn_nnn'nTGG
Cuts at: 0 1043 2191 2415
Size: 1043 1148 224
Fragments arranged by size:
1148 1043 224
PfoI T'CCnGG_A
Cuts at: 0 2119 2376 2415
Size: 2119 257 39
Fragments arranged by size:
2119 257 39
PpuMI rG'GwC_Cy
Cuts at: 0 1047 1553 2415
Size: 1047 506 862
Fragments arranged by size:
1047 862 506
PshAI GACnn'nnGTC
Cuts at: 0 1829 2415
Size: 1829 586
PsiI TTA'TAA
Cuts at: 0 149 2415
Size: 149 2266
PstI C_TGCA'G
Cuts at: 0 293 1236 2415
Size: 293 943 1179
Fragments arranged by size:
1179 943 293
PvuII CAG'CTG
Cuts at: 0 288 525 2415
Size: 288 237 1890
Fragments arranged by size:
1890 288 237
SacII CC_GC'GG
Cuts at: 0 657 2415
Size: 657 1758
SapI GCTCTTCn'nnn_
Cuts at: 0 15 302 2415
Size: 15 287 2113
Fragments arranged by size:
2113 287 15
ScaI AGT'ACT
Cuts at: 0 384 1960 2415
Size: 384 1576 455
Fragments arranged by size:
1576 455 384
SexAI A'CCwGG_T
Cuts at: 0 435 2415
Size: 435 1980
SfcI C'TryA_G
Cuts at: 0 289 378 648 1232 1640 2415
Size: 289 89 270 584 408 775
Fragments arranged by size:
775 584 408 289 270 89
SmlI C'TyrA_G
Cuts at: 0 971 2415
Size: 971 1444
SspI AAT'ATT
Cuts at: 0 159 920 2415
Size: 159 761 1495
Fragments arranged by size:
1495 761 159
StuI AGG'CCT
Cuts at: 0 901 2415
Size: 901 1514
TatI w'GTAC_w
Cuts at: 0 382 833 1958 2415
Size: 382 451 1125 457
Fragments arranged by size:
1125 457 451 382
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2243 2275 2415
Size: 2243 32 140
Fragments arranged by size:
2243 140 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 81 1207 2415
Size: 81 1126 1208
Fragments arranged by size:
1208 1126 81
XmnI GAAnn'nnTTC
Cuts at: 0 716 1660 2415
Size: 716 944 755
Fragments arranged by size:
944 755 716
Enzymes that do cut:
AcuI AflIII AhdI AleI AlwNI ApaLI ApoI BamHI
BanII BbsI BbvCI BciVI BglII Bme1580I BmrI BpmI
Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BsrDI BstAPI BstYI BtgI BtsI ClaI
DraI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI Hin4I HindIII MfeI MslI MspA1I NdeI NspI
PciI PflMI PfoI PpuMI PshAI PsiI PstI PvuII
SacII SapI ScaI SexAI SfcI SmlI SspI StuI
TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AlfI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BanI BbeI BcgI BclI BfrBI BglI BlpI
BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
CspCI DraIII DrdI EagI EciI EcoICRI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MluI MmeI
MscI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PmlI PpiI PspOMI PspXI
PsrI PvuI RsrII SacI SalI SanDI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StyI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI